Optimization of enzymatic transesterification of rapeseed oil ester using response surface and principal component methodology

Uosukainen, Esa; Lämsä, Merja; Linko, Yu‐Yen; Linko, P.; Leisola, Matti

doi:10.1016/s0141-0229(99)00034-4

Cited by 43 publications

(17 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, a study was realized using RSM in combination with principal component analysis methods for optimizing the enzymatic transesterification of rapeseed oil methyl esters. 11 Demirkol et al 7 reported the optimization of enzymatic methanolysis of soybean oil by RSM using Lipozyme RM IM.…”

Section: Introductionmentioning

confidence: 99%

Lipase‐catalyzed ethanolysis of soybean oil in a solvent‐free system using central composite design and response surface methodology

Rodrigues

Volpato

Ayub

et al. 2008

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND: In this work we describe the synthesis of ethyl esters, commonly known as biodiesel, using refined soybean oil and ethanol in a solvent-free system catalyzed by lipase from Thermomyces lanuginosus. Central composite design and response surface methodology (RSM) were employed to optimize the biodiesel synthesis parameters, which were: reaction time, temperature, substrate molar ratio, enzyme content, and added water, measured as percentage of yield conversion.

show abstract

Section: Introductionmentioning

confidence: 99%

Lipase‐catalyzed ethanolysis of soybean oil in a solvent‐free system using central composite design and response surface methodology

Rodrigues

Volpato

Ayub

et al. 2008

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

show abstract

“…Soybean oil esters were used in the USA (Muniyappa et al, 1996;Raneses et al, 1999), rape-seed oil esters in Europe (Uosukainen et al, 1999;Janulis, 2004), palm oil esters in Malaysia (Kalam and Masjuki, 2002;Al-Widyan and Al-Shyoukh, 2002) and recycled restaurant greases in Japan Demirbas, 2007). However, in many developing countries, especially China, soybean oil is consumed as a main edible oil resource, and palm oil is imported on the whole and has higher prices (1 US$/kg).…”

Section: Introductionmentioning

confidence: 99%

Lipase-Catalyzed Biodiesel Production with Methyl Acetate as Acyl Acceptor

Huang

Yan

2008

Zeitschrift Für Naturforschung C

View full text Add to dashboard Cite

Biodiesel is an alternative diesel fuel made from renewable biological resources. During the process of biodiesel production, lipase-catalyzed transesterification is a crucial step. However, current techniques using methanol as acyl acceptor have lower enzymatic activity; this limits the application of such techniques in large-scale biodiesel production. Furthermore, the lipid feedstock of currently available techniques is limited. In this paper, the technique of lipase-catalyzed transesterification of five different oils for biodiesel production with methyl acetate as acyl acceptor was investigated, and the transesterification reaction conditions were optimized. The operation stability of lipase under the obtained optimal conditions was further examined. The results showed that under optimal transesterification conditions, both plant oils and animal fats led to high yields of methyl ester: cotton-seed oil, 98%; rapeseed oil, 95%; soybean oil, 91%; tea-seed oil, 92%; and lard, 95%. Crude and refined cottonseed oil or lard made no significant difference in yields of methyl ester. No loss of enzymatic activity was detected for lipase after being repeatedly used for 40 cycles (ca. 800 h), which indicates that the operational stability of lipase was fairly good under these conditions. Our results suggest that cotton-seed oil, rape-seed oil and lard might substitute soybean oil as suitable lipid feedstock for biodiesel production. Our results also show that our technique is fit for various lipid feedstocks both from plants and animals, and presents a very promising way for the large-scale biodiesel production.

show abstract

“…Enzymatic transesterification has shown to be a good alternative at laboratory scale, and it might be scaled-up for bioethanol plants. Enzymatic reactions using lipases have been reported to give good yields of methyl esters with different raw materials (Mukherjee, 1988, Breivik et al, 1997, Shimada et al, 1997, Shimada et al, 1998, Uosukainen et al, 1999, Wu et al, 1999, Vacek et al, 2000, Soumanou and Bornscheuer, 2003, Shah et al, 2004, Zhang et al, 2003.…”

Section: New Trendsmentioning

confidence: 99%

Production of biodiesel from vegetable oils

Cerveró¹,

Coca²,

Luque³

2008

Grasas y Aceites

View full text Add to dashboard Cite

RESUMEN Producción de biodiésel a partir de aceites vegetalesEl biodiésel se produce mediante la transesterificación de triglicéridos, presentes en grasas animales o aceites vegetales, en un proceso en el que un alcohol de bajo peso molecular desplaza a la glicerina. La mezcla de esteres así resultante posee unas propiedades físico-químicas similares a las del diésel procedente de petróleo. En este artículo se revisan las vías de síntesis de biodiésel mediante la transesterificación catalítica de aceites vegetales. Aunque actualmente a escala industrial solo se producen ésteres metí-licos, también se ha considerado el uso de etanol, ya que éste se obtiene también de fuentes renovables, generando así un combustible más limpio y biocompatible. PALABRAS-CLAVE: Biodiésel -Ésteres de ácidos grasos -Transesterificación -Triglicéridos. SUMMARY Production of biodiesel from vegetable oilsBiodiesel is produced by transesterification of triglycerides present in animal fat or vegetable oils, by displacing glycerine with a low molar mass alcohol. This resulting ester mixture has physico-chemical properties similar to those of petroleum diesel. This paper reviews the synthetic paths that lead to biodiesel by means of the catalytic transesterification of vegetable oils. Although methyl esters are at present the only ones produced at industrial scale, the use of ethanol, which can also be obtained from renewable resources, has been considered, since it would generate a cleaner and more biocompatible fuel.

show abstract

Optimization of enzymatic transesterification of rapeseed oil ester using response surface and principal component methodology

Cited by 43 publications

References 12 publications

Lipase‐catalyzed ethanolysis of soybean oil in a solvent‐free system using central composite design and response surface methodology

Lipase‐catalyzed ethanolysis of soybean oil in a solvent‐free system using central composite design and response surface methodology

Lipase-Catalyzed Biodiesel Production with Methyl Acetate as Acyl Acceptor

Production of biodiesel from vegetable oils

Contact Info

Product

Resources

About