Optimization of Enzymatic Saccharification of Alkali Pretreated Typha angustifolia for Glucose Production

Abstract

Abstract-Lignocellulose ethanol is significantly sustainable bio-fuel. It is an environmentally friendly. This work is to develop a hydrolysis process of pretreated lignocellulose to ethanol production from narrow leaved cattail as a biomass material. Response surface methodology (RSM) with a central composite design (CCD) was followed to optimize the enzymatic saccharification process in order to obtain high glucose yield. Three independent variables (cellulase, β-glucosidase and temperature) that operating condition, vary at CCD five levels (-α, -1, 0, +1, +α). The optimum values for the predicted variables for glucose and xylose released were: cellulase loading 17.5 FPU/g substrate, β-glucosidase loading 0 U/g substrate and hydrolysis temperature 55°C. Under these optimal conditions, glucose and xylose yield reached to 413.25 mg/ g substrate and 75.48 mg/g substrate, respectively. The results of a confirmation experiment under the optimum conditions agreed with model predictions.

Solid state fermentation and crude cellulase based bioconversion of potential bamboo biomass to reducing sugar for bioenergy production

“…Optimization of enzymatic saccharification using RSM was also reported in previous studies . Sopajarn and Sangwichien used CCD in RSM to optimize the cellulase saccharification process in order to obtain high glucose from cattail material (wetland plants) for bioethanol production. Furthermore, other studies have also followed RSM for optimization of various pretreatment and saccharification conditions .…”

“…Sopajarn and Sangwichien used CCD in RSM to optimize the cellulase saccharification process in order to obtain high glucose from cattail material (wetland plants) for bioethanol production. Furthermore, other studies have also followed RSM for optimization of various pretreatment and saccharification conditions . Experimental validation under our predicted optimal conditions yielded 47.19% saccharification (Table ) of fungal pretreated biomass.…”

“…Furthermore, other studies have also followed RSM for optimization of various pretreatment and saccharification conditions. 5,38,42,44 Experimental validation under our predicted optimal conditions yielded 47.19% saccharification (Table 7) of wileyonlinelibrary.com/jsfa fungal pretreated biomass. Thus overall good agreement was observed between experimentally determined (observed) and predicted responses.…”

From Laboratory Tests to the Ecoremedial System: The Importance of Microorganisms in the Recovery of PPCPs-Disturbed Ecosystems

“…Although phytoremediation is an excellent biotechnology strategy in soil and water systems, it does not recover 100% of the contaminated area and requires that prior to planting, complementary methods should be used to reduce the amount of pollutants in the area. Additionally, when selecting a plant for phytoremediation, it is necessary to consider its physiological requirements for growth, its condition as a native or introduced plant [261], as well as its effectiveness against a specific pollutant, as well as the secondary added value of the plants (Figure 5), for example, ornamental uses or primary biomass to produce bioethanol [255,258,262]. Hitherto, studies have focused on the effectiveness of recovery processes, the detection of ultra-trace substances along with the identification of metabolites that are produced in the plant upon treatment with active pharmaceutical ingredients [263,264] and in the understanding of metabolic pathways associated with their degradation [265].…”

Optimization of olive pomace enzymatic hydrolysis for fermentable sugar production

“…For the conversion of lignocellulosic biomass into ethanol, it is necessary to understand the relationship between the recalcitrance of biomass and pretreatment and enzymatic hydrolysis. Furthermore, to facilitate the bioconversion and to achieve a commercial conversion potential, it is important to improve the pretreatment technologies, to optimize the enzymatic hydrolysis process and to develop better fermentation processes (Singh and Trivedi, 2013;Sopajarn and Sangwichien, 2015;Sukri et al, 2014). Pretreatment process is the initial step to degrade the biomass content.…”