Optimization of enzymatic hydrolysis of cellulosic fraction obtained from stranded driftwood feedstocks for lipid production by Solicoccozyma terricola

Tasselli, Giorgia; Filippucci, Sara; D’Antonio, Silvia; Cavalaglio, Gianluca; Turchetti, Benedetta; Cotana, Franco; Buzzini, Pietro

doi:10.1016/j.btre.2019.e00367

Cited by 4 publications

(3 citation statements)

References 44 publications

(60 reference statements)

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“…Most of the fungal reads from the rhizosphere soil belonged to S. terrea , but no information is available on this fungal species. The other Solicoccozyma species found in this study, S. terricola is a well known psychrotolerant yeast used in lipid production ( Filippucci et al, 2016 ; Stosiek et al, 2019 ; Tasselli et al, 2019 ). Lastly, Mortierella camargensis isolated from grassland soils showed ability to produce arachidonic acid and lipase activity ( Botha et al, 1999 ; Miklós et al, 2012 ).…”

Section: Discussionmentioning

confidence: 89%

The Effects of Host Plant Genotype and Environmental Conditions on Fungal Community Composition and Phosphorus Solubilization in Willow Short Rotation Coppice

et al. 2021

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Phosphorus (P) is an essential plant nutrient. Low availability of P in soil is mainly caused by high content of Fe2O3 in the clay fraction that binds to P making it unavailable. Beneficial microbes, such as P solubilizing microorganisms can increase the available P in soil and improve plant growth and productivity. In this study, we evaluated the effects of environmental conditions (climate, soil parameters), plant genotype, and level of plant association (rhizosphere or endophytic root organism) on the abundance and diversity of phosphorus solubilizing microorganisms in a Salix production system. We hypothesized that a lower number of endophytic fungi may possess the ability to solubilize P compared to the number of rhizosphere fungi with the same ability. We also expect that the plant genotype and the experimental site with its environmental conditions will influence fungal diversity. Two Salix genotypes grown in pure and mixed cultures were investigated for their fungal microbiome community and diversity in the rhizosphere and endosphere during two growing seasons. We found that the rhizosphere fungal community was more diverse. A general dominance of Ascomycota (Dothideomycetes) and Basidiomycota (Tremellomycetes) was observed. The classes Agaricomycetes and Pezizomycetes were more frequent in the endosphere, while Tremellomycetes and Mortierellomycetes were more abundant in the rhizosphere. Plot-specific soil properties (pH, total organic carbon, and nitrogen) significantly influenced the fungal community structure. Among the culturable fungal diversities, 10 strains of phosphate solubilizing fungi (PSFs) from roots and 12 strains from rhizosphere soil were identified using selective media supplemented with di-calcium and tri-calcium phosphates. The fungal density and the number of PSF were much higher in the rhizosphere than in the endosphere. Penicillium was the dominant genus of PSF isolated from both sites; other less frequent genera of PSFs were Alternaria, Cladosporium, and Clonostachys. Overall the main factors controlling the fungal communities (endophytic vs. rhizosphere fungi) were the soil properties and level of plant association, while no significant influence of growing season was observed. Differences between Salix genotypes were observed for culturable fungal diversity, while in metagenomic data analysis, only the class Dothideomycetes showed a significant effect from the plant genotype.

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Section: Discussionmentioning

confidence: 89%

The Effects of Host Plant Genotype and Environmental Conditions on Fungal Community Composition and Phosphorus Solubilization in Willow Short Rotation Coppice

et al. 2021

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“…Experiments were carried out in Erlenmeyer flasks (50 ml) containing sodium citrate buffer (50 mM; 20 ml) and sodium azide (2%) to prevent microbial contamination. All the parameters like substrate concentration, pH, temperature and enzyme dosage range were setup using RSM [ 25 ]. After 24 h of enzymatic saccharification, the samples were taken from the fermentable broth and centrifuged at 10,000× g for 5 min, the supernatant withdrawn for analysis of reducing sugars.…”

Section: Methodsmentioning

confidence: 99%

Optimization of oxalic acid pre-treatment and enzymatic saccharification in Typha latifolia for production of reducing sugar

Ramaiah

Thimappa

Nataraj

2020

Journal of Genetic Engineering and Biotechnology

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Background Plants with high biomass can be manipulated for their reducing sugar content which ultimately upon fermentation produces ethanol. This concept was used to enhance the production of reducing sugar from cattail ( Typha latifolia ) by oxalic acid (OAA) pre-treatment followed by enzymatic saccharification. Result The optimum condition of total reducing sugar released from OAA pre-treatment was found to be 22.32 mg/ml (OAA—1.2%; substrate concentration (SC)—6%; reaction time (RT)—20 min) using one variable at a time (OVAT). Enzymatic saccharification yielded 45.21 mg/ml of reducing sugar (substrate concentration (SC)—2.4%; enzymatic dosage—50 IU/g; pH 7.0; temp—50 °C) using response surface methodology (RSM). Conclusion We conclude that Typha can be used as a potential substrate for large-scale biofuel production, employing economical bioprocessing strategies.

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“…Com isso, após seleção das melhores condições de obtenção do coquetel a partir do cocultivo de M. thermophilus e T. reesei RP698 em PM a 35 ºC, foi realizado o escalonamento para biorreator BioFlo 310 com capacidade de trabalho de 5 L. O coquetel obtido foi aplicado nos três tipos de canas estudadas e foram testadas diferentes variáveis: temperatura, pH, concentração proteica do coquetel, tempo de hidrólise, carga de sólidos e o efeito de surfactantes. A temperatura de 50 ºC determinada nos experimentos está de acordo a descrita para a maioria dos coquetéis e enzimas associados a hidrólise de biomassa lignocelulósica como foi mostrado nos experimentos realizados por Goldbeck et al (2016) (51), Arias et al (2016) (146) e Tasselli et al (2019)(149). Apesar da melhor temperatura de ação do coquetel ter sido 50 ºC, é possível trabalhar na faixa de 40 a 55 ºC, uma vez que o coquetel apresentou bons resultados de sacarificação na faixa citada.…”

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Cultivo de Mycothermus thermophilus e Trichoderma reesei RP698 em resíduos agroindustriais: consórcio microbiano no desenvolvimento de um coquetel enzimático para sacarificação de cana-de-açúcar

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Optimization of enzymatic hydrolysis of cellulosic fraction obtained from stranded driftwood feedstocks for lipid production by Solicoccozyma terricola

Cited by 4 publications

References 44 publications

The Effects of Host Plant Genotype and Environmental Conditions on Fungal Community Composition and Phosphorus Solubilization in Willow Short Rotation Coppice

The Effects of Host Plant Genotype and Environmental Conditions on Fungal Community Composition and Phosphorus Solubilization in Willow Short Rotation Coppice

Optimization of oxalic acid pre-treatment and enzymatic saccharification in Typha latifolia for production of reducing sugar

Cultivo de Mycothermus thermophilus e Trichoderma reesei RP698 em resíduos agroindustriais: consórcio microbiano no desenvolvimento de um coquetel enzimático para sacarificação de cana-de-açúcar

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