2012
DOI: 10.1631/jzus.b1100297
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Optimization of enzymatic hydrolysis of cassava to obtain fermentable sugars

Abstract: This work evaluates the enzymatic hydrolysis of starch from cassava using pectinase, α-amylase, and amyloglucosidase. A central composite rotational design (CCRD) was carried out to evaluate the effects of amyloglucosidase, pectinase, reaction time, and solid to liquid ratio. All the experiments were carried out in a bioreactor with working volume of 2 L. Approximately 98% efficiency hydrolysis was obtained, resulting in a concentration of total reducing sugar released of 160 g/L. It was concluded that pectina… Show more

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Cited by 31 publications
(19 citation statements)
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“…Previous studies on cassava starch focused on starch-hydrolyzing enzymes such as α-amylase, amyloglucosidase and pectinase to achieve maximum hydrolytic efficiency of about 98%, resulting in 160 g/L of total reducing sugar [60], although this seems not have a direct application in processing cassava or its products for poultry feeding. However, this processing method has potential for use in the poultry feed industry.…”
Section: Chemical Processing Of Cassava For Poultry Feedingmentioning
confidence: 99%
“…Previous studies on cassava starch focused on starch-hydrolyzing enzymes such as α-amylase, amyloglucosidase and pectinase to achieve maximum hydrolytic efficiency of about 98%, resulting in 160 g/L of total reducing sugar [60], although this seems not have a direct application in processing cassava or its products for poultry feeding. However, this processing method has potential for use in the poultry feed industry.…”
Section: Chemical Processing Of Cassava For Poultry Feedingmentioning
confidence: 99%
“…Cassava is rich in starch, with competing demand as industrial raw material as well as food. Previous studies on cassava starch focused on starch hydrolysing enzymes such as α-amylase, amyloglucosidase, and pectinase to achieve maximum hydrolysis efficiency of about 98 %, resulting in 160 g/L of total reducing sugar (Collares et al 2012 ). Recently, Olanbiwoninu and Odunfa ( 2015 ) hydrolysed cassava peel into fermentable sugars using organic acid pre-treatment before enzyme hydrolysis.…”
Section: Introductionmentioning
confidence: 99%
“…Pectinases are also being used in biorefineries for hydrolyzing pectin present in pectin-rich agro-industrial wastes (Biz et al 2014 ). These wastes are processed into simple sugars so that they could be converted into bioethanol or used as fermentable sugars (Collares et al 2012 ; Hossain et al 2011 ). Different enzymes such as pectinase, hemicellulases and cellulases are being used to convert polysaccharides present in the plant cell wall into simple sugars (Beldman et al 1984 ).…”
Section: Industrial Applicationsmentioning
confidence: 99%