Optimization of Endo-Pectinase and Pectin Lyase Production from Wheat Bran by Bacillus pumilus using Response Surface Methodology

Tepe, Özlem; Dursun, Arzu

doi:10.35378/gujs.808326

Cited by 3 publications

(1 citation statement)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Figure 3 presents perturbation plots showing the effect of each of the independent variables on the IC. It shows that the initial concentration (A), adsorbent dose (B) and time (C) are highly influenced by the operating parameters of IC adsorption (Tepe and Dursun, 2021;Sadhukhan, et al, 2016).…”

Section: Response Surfaces and Contour Plots For Indigo Carmine Adsor...mentioning

confidence: 99%

Modelling and Optimization of Indigo Carmine Adsorption onto P(NIPAM-co-AN)/Clay Composite Using Response Surface Methodology

Pıhtılı

Tanyol

2022

International Journal of Pure and Applied Sciences

View full text Add to dashboard Cite

Bu çalışmada yeni bir polimerik adsorban olan Poli(NIPAM-co-AN)/kil kompoziti yerinde polimerizasyon ile hazırlanmıştır. N-izopropilakrilamid, akrilonitril ve uygun miktarda alınan kilin (yaklaşık %5 w/w) kopolimerizasyonu, 10 mL 1,4-dioksan varlığında 65°C'de, AIBN (azobisizobütironitril) başlatıcısı varlığında gerçekleştirildi. NIPAM-AN/kil kompoziti IC (indigo karmin) boyasını sentetik atık sulardan uzaklaştırmak için yeni bir adsorban olarak kullanılmıştır. Üç önemli proses parametresi olan başlangıç indigo karmin konsantrasyonu (5-30 mg/L), adsorban dozu (10-80 mg) ve temas süresi (10-180 dakika) yanıt yüzeyi metodolojisi (RSM) ile birleştirilmiş. Merkezi Kompozit Tasarım (CCD) kullanılarak modelleme yapıldı ve en iyi indigo karmin giderme yanıtını elde etmek için optimize edildi. Çözeltideki indigo karmin miktarı, 650 nm dalga boyunda bir UV-görünür spektrofotometre kullanılarak ölçülmüştür. ANOVA (varyans analizi) istatistiksel değerine dayalı olarak, indigo karminin P(NIPAM-co-AN)/kil kompoziti üzerine adsorpsiyonu, çok düşük olasılık değeriyle (p

show abstract

Section: Response Surfaces and Contour Plots For Indigo Carmine Adsor...mentioning

confidence: 99%

Modelling and Optimization of Indigo Carmine Adsorption onto P(NIPAM-co-AN)/Clay Composite Using Response Surface Methodology

Pıhtılı

Tanyol

2022

International Journal of Pure and Applied Sciences

View full text Add to dashboard Cite

show abstract

The Realm of Smart Biomass Degrading Enzymes in Low-Carbon Fuels and Chemicals Production

Katarina,

Ilić,

Milić

2024

Biorefinery and Industry 4.0: Empowering Sustainability

View full text Add to dashboard Cite

Optimization of substrate composition for pectinase production from Satkara (Citrus macroptera) peel using Aspergillus niger-ATCC 1640 in solid-state fermentation

Ahmed

Rana

Zzaman

et al. 2021

Heliyon

View full text Add to dashboard Cite

Pectinase is an enzyme having a broad industrial and commercial application. However, higher production costs may be the major constraint for the wide-scale application of pectinase. Therefore, researchers are trying to reduce the pectinase production cost for subsequent application in the industrial processes by using a unique substrate and optimizing the fermentation medium components and process conditions. The main purpose of the current study was to optimize medium composition for pectinase production using Aspergillus niger -ATCC 1640 in the solid-state fermentation. The Response Surface Methodology (RSM) was performed to evaluate the effects of variables, specifically the concentrations of Satkara peel, urea, (NH 4 ) 2 PO 4 , NH 4 NO 3 , KH 2 PO 4 , ZnSO 4 , and MgSO 4 .7H 2 O on pectinase production in the solid substrate. Firstly, a two-factorial design, Plackett-Burman design (PBD) was applied to screen the variables that significantly influenced the pectinase production. After finding the critical variables, 15 experimental runs were carried out using a Box-Behnken design (BBD) to derive a statistical model for optimizing the concentrations of the selected variables. The PBD model revealed that Satkara peel, urea, and (NH 4 ) 2 SO 4 significantly affected the pectinase production. RSM results indicated that the predicted response for pectinase production was in good agreement with experimental data (R 2 = 0.9836). Under the optimized condition of Satkara peel (8.4 g/L), urea (0.5 g/L), and (NH 4 ) 2 SO4 (2.7 g/L), the pectinase activity was predicted to be 0.6178 μmol/mL. In the present study, the experimental pectinase production achieved 0.6045 μmol/mL. The study revealed that optimization through RSM could improve the pectinase production from Satkara peel.

show abstract

Optimization of Endo-Pectinase and Pectin Lyase Production from Wheat Bran by Bacillus pumilus using Response Surface Methodology

Abstract: Highlights• This paper focuses on pectin lyase and endo-pectinase synthesis from wheat bran by Bacillus pumilus.• RSM was employed.• Optimum conditions for enzyme production were obtained.

Cited by 3 publications

References 33 publications

Modelling and Optimization of Indigo Carmine Adsorption onto P(NIPAM-co-AN)/Clay Composite Using Response Surface Methodology

Modelling and Optimization of Indigo Carmine Adsorption onto P(NIPAM-co-AN)/Clay Composite Using Response Surface Methodology

The Realm of Smart Biomass Degrading Enzymes in Low-Carbon Fuels and Chemicals Production

Optimization of substrate composition for pectinase production from Satkara (Citrus macroptera) peel using Aspergillus niger-ATCC 1640 in solid-state fermentation

Contact Info

Product

Resources

About