Optimization of dissolved organic nitrogen (DON) measurements in aqueous samples with high inorganic nitrogen concentrations

Vandenbruwane, Jeroen; Neve, Stefaan De; Qualls, Robert G.; Salomez, Joost; Hofman, Georges

doi:10.1016/j.scitotenv.2007.06.025

Cited by 58 publications

(47 citation statements)

References 27 publications

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“…This is supported by the strong increase of fluvial DON concentrations in catchments with intensive agriculture, which was found in a recent study when using the direct measurement of DON concentrations with SEC (Heinz et al, 2015). We recommend the use of the novel direct measurement technique shown in this and other studies (Graeber et al, 2012a;Heinz et al, 2015), or that treatments to remove nitrate and ammonium before indirect determination of DON (Lee and Westerhoff, 2005;Vandenbruwane et al, 2007;Chon et al, 2013;Graeber et al, 2012c) should be used in future studies to re-assess the effects of agriculture on DON concentrations in soils and in streams.…”

Section: Effects Of Farming Intensity On Fluvial Dom Quantitysupporting

confidence: 74%

“…The disparity of results between soil and catchment-scale studies on the effects of agriculture on DON concentrations may be a result of DON measurement problems, as was clearly stated in some soil DON studies (Siemens and Kaupenjohann, 2002;Solinger et al, 2001). In detail, the indirect determination of DON as the difference between total dissolved nitrogen and dissolved inorganic nitrogen can result in severe miscalculations of DON concentrations in high-nitrate environments (Graeber et al, 2012a;Lee and Westerhoff, 2005;Vandenbruwane et al, 2007). Therefore, we propose that the differences in results between soil and stream studies are an artefact of the indirect determination of DON.…”

Section: Effects Of Farming Intensity On Fluvial Dom Quantitymentioning

confidence: 99%

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Interacting effects of climate and agriculture on fluvial DOM in temperate and subtropical catchments

Graeber

Goyenola

Meerhoff

et al. 2015

Hydrol. Earth Syst. Sci.

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Abstract. Dissolved organic matter (DOM) is an important factor in aquatic ecosystems, which is involved in a large variety of biogeochemical and ecological processes, and recent literature suggests that it could be strongly affected by agriculture in different climates. Based on novel monitoring techniques, we investigated the interaction of climate and agriculture effects on DOM quantity and quality. To examine this, we took water samples over 2 years in two paired intensive and extensive farming catchments in each of Denmark (temperate climate) and Uruguay (subtropical climate). We measured dissolved organic carbon (DOC) and nitrogen (DON) concentrations and DOC and DON molecular fractions with size-exclusion chromatography. Moreover, we characterized DOM quality with absorbance and fluorescence measurements, as well as parallel factor analysis (PARAFAC). We also calculated the DOC and DON loads based on daily discharge measurements, as well as measured precipitation and air temperature. The fluvial DOM in the catchments in Uruguay was characterized by higher temporal variability of DOC and DON loads which were clearly to a higher temporal variability of precipitation and a DOM composition with rather plant-like character relative to the Danish catchments. Moreover, we found a consistently higher temporal variability of DOC and DON loads in the intensive farming catchments than in the extensive farming catchments, with highest temporal variability in the Uruguayan intensive farming catchment. Furthermore, the composition of DOM exported from the intensive farming catchments was consistently complex and always related to microbial processing in both Denmark and Uruguay. This was indicated by low C : N ratios, several spectroscopic DOM composition indices and PARAFAC fluorescence components. We propose that the consistent effect of intensive farming on DOM composition and the temporal variability of DOC and DON loads is related to similarities in the management of agriculture, which may have widescale implications for fluvial DOM composition, as well as related ecological processes and biogeochemical cycles.

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Section: Effects Of Farming Intensity On Fluvial Dom Quantitysupporting

confidence: 74%

Section: Effects Of Farming Intensity On Fluvial Dom Quantitymentioning

confidence: 99%

Interacting effects of climate and agriculture on fluvial DOM in temperate and subtropical catchments

Graeber

Goyenola

Meerhoff

et al. 2015

Hydrol. Earth Syst. Sci.

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“…Furthermore, DON analysis will be challenging if, as in our case, high concentrations of dissolved inorganic nitrogen are present as errors of the analytical methods may add up and as total nitrogen analysis is prone to relatively low recovery rates under these conditions (e.g. Graeber et al, 2012;Vandenbruwane et al, 2007). Therefore, we had to accept some uncertainties in the DON measurements which we cannot quantify.…”

Section: Controls On Doc and N Dynamics In The Ditchmentioning

confidence: 98%

Nitrogen and dissolved organic carbon (DOC) losses from an artificially drained grassland on organic soils

Tiemeyer¹,

Kahle

2014

Biogeosciences

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Abstract. -N) as well as dissolved organic carbon (DOC) and nitrogen (DON) concentrations and losses were studied for three and two years, respectively, in a small catchment dominated by a degraded peatland used as intensive grassland. Concentrations in the shallow groundwater were spatially and temporally very variable, with NO 3 -N being the most dynamic component . Ditch DOC concentration were lower than the groundwater DOC concentration of 50.6 ± 15.2 mg L −1 (14.9 to 88.5 mg L −1 ). Both DOC and N concentrations were governed by hydrological conditions, but NO 3 -N reacted much faster and clearer on rising discharge rates than DOC, which tended to be higher under drier conditions. In the third year of the study, the superposition of a very wet summer and land use changes from grassland to arable land in a part of the catchment suggests that, under re-wetting conditions with a high groundwater table in summer, NO 3 -N would diminish quickly, while DOC would remain on a similar level. Further intensification of the land use, on the other hand, would increase N losses to receiving water bodies.

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“…The only way to determine the amount of organic nitrogen in an aqueous sample is to measure separately amounts of nitrate, ammonium and total nitrogen and to calculate the amount of organic nitrogen as the difference between the total nitrogen and inorganic nitrogen concentrations in the sample. The subtraction of independently measured concentrations results in the accumulation of analytical variance in organic nitrogen determination as arises from the error propagation law (Vandebruwane et al 2007). The precision of the dissolved organic nitrogen (DON) measurement also depends on the inorganic nitrogen concentrations being lower when samples with high inorganic nitrogen concentrations are processed (Hansell 1993).…”

Section: Institute Of Forest Ecology Faculty Of Forestry and Wood Tementioning

confidence: 99%

Organic and inorganic nitrogen in precipitation and in forest throughfall at Bílý Kříž site (Beskydy Mts., Czech Republic)

Drápelová¹

2012

J. For. Sci.

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Organic nitrogen is an important but yet not very well explored component of nitrogen deposition. In this study concentrations and fluxes of organic and inorganic nitrogen in bulk precipitation in an open field (BOF) and in throughfall (THR) were evaluated at the Bílý Kříž experimental site (Moravian-Silesian Beskydy Mts., Czech Republic, 908 m a.s.l.) with a young Norway spruce stand. The results of a two-year study (2008 and 2009) were compared with the results obtained during the same time period on forest plots included in ICP Forests Programme in the Czech Republic. Total nitrogen deposition in BOF at the Bílý Kříž site amounted to about 918 mg·m–2·a–1, the contribution of organic nitrogen was about 8%. Total nitrogen flux with THR at Bílý Kříž was about 1,305 mg·m–2·a–1 during the studied years and organic N accounted for 12% of this amount. The ranges of the two-year average values found for total nitrogen flux on ICP Forests plots throughout the Czech Republic were as follows: 759–1,857 mg N·m–2·a–1 with 7–38% contribution of organic N in BOF and 928–3,816 mg N·m–2·a–1 with 7–20% contribution of organic N in THR. The share of organic nitrogen in THR nitrogen fluxes at Bílý Kříž proved clear seasonality with maxima in July. A highly significant correlation between N-NH4+   and N-NO3–   concentrations in BOF suggested the common anthropogenic source of these substances at the Bílý Kříž site. No significant correlation was found either between organic N and N-NH4+ or between organic N and N-NO3– concentrations in BOF. Cumulative deposition charts showed different behaviour of particular nitrogen deposition components while passing through the canopy.

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Optimization of dissolved organic nitrogen (DON) measurements in aqueous samples with high inorganic nitrogen concentrations

Cited by 58 publications

References 27 publications

Interacting effects of climate and agriculture on fluvial DOM in temperate and subtropical catchments

Interacting effects of climate and agriculture on fluvial DOM in temperate and subtropical catchments

Nitrogen and dissolved organic carbon (DOC) losses from an artificially drained grassland on organic soils

Organic and inorganic nitrogen in precipitation and in forest throughfall at Bílý Kříž site (Beskydy Mts., Czech Republic)

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