Optimization of Disk Sorptive Extraction Based on Monolithic Material for the Determination of Aroma Compounds from Lantana camara L. by Gas Chromatography-Mass Spectrometry

Jang, Hyejin; Son, Hyun-Hwa; Lee, Dong‐Sun

doi:10.5012/bkcs.2011.32.12.4275

Cited by 14 publications

(10 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Monotrap is a disposable sorptive media based on the large surface area of silica monolith. It has been applied in biological research and can be preserved in the sample bottle prior to measurement (Jang et al, 2011 ; Ma et al, 2013 ). Nonetheless, despite the large surface for capturing VOCs, monotrap has been used solely for static sampling.…”

Section: Introductionmentioning

confidence: 99%

Elucidation of Biochemical Pathways Underlying VOCs Production in A549 Cells

Furuhashi¹,

Ishii²,

H³

et al. 2020

Front. Mol. Biosci.

View full text Add to dashboard Cite

Cellular volatile organic compounds (VOCs) are unique compounds whose metabolic pathways remain enigmatic. To elucidate their metabolism, we investigated the VOCs of lung cancer A549 and 2 non-cancer lung cells (HLB; HBEpC). Neutral sugars and lactate in the medium were measured by colorimetric assay. VOCs were enriched by monotrap and profiled by GC-MS. To investigate the enzymes that change VOC metabolism in cells, we conducted ALDH activity assays and qPCR. ROS (reactive oxygen species) assays were conducted to assess oxidation stress. The colorimetric assay showed that especially A549 and HLB took up sugars from the medium and rapidly secreted lactate into the medium. The VOC profile (GC-MS) revealed a trans-2-hexenol increase, especially in A549 lung cancer cells. This is a novel lipid peroxidation product from animal cells. Based on the absolute quantification data, trans-2-hexenol increased in parallel with number of A549 cancer cells incubated. The qPCR data implies that ADH1c potentially plays an important role in the conversion into trans-2-hexenol.

show abstract

Section: Introductionmentioning

confidence: 99%

Elucidation of Biochemical Pathways Underlying VOCs Production in A549 Cells

Furuhashi¹,

Ishii²,

H³

et al. 2020

Front. Mol. Biosci.

View full text Add to dashboard Cite

show abstract

“…It has a high surface area chemically modified with octadecyl silane, was launched as a new sampling pretreatment technique (Wu, Tao, & Gu, ). It is superior to SPME fiber, because of its larger aperture and specific surface area (Jang, Son, & Lee, ), therefore, it is more sensitive and has higher adsorption ratio than HS‐SPME. Thus the headspace monolithic material sorptive extraction (HS‐MMSE) method is an alternative sampling technique for SPME (Gilart, Cormack, Marcé, Borrull, & Fontanals, ).…”

Section: Introductionmentioning

confidence: 99%

Volatile components present in different parts of grass carp

et al. 2018

View full text Add to dashboard Cite

This study examined the dorsal meat, belly meat, red meat, skin, intestines and gills of grass carp to determine the volatile compounds present in different parts using the monolithic material sorptive extraction gas chromatography–mass spectrometry and the electronic nose. Although the electronic nose was able to distinguish the flavors of the red meat, skin, intestines and gills, the dorsal and belly meat could not be differentiated. For dorsal meat, belly meat, red meat, fish skin, fish intestines and fish gills, 56, 55, 58, 67, 59, and 68 volatile compounds, respectively, were identified, the majority of which were aldehydes and alcohols. There were 9, 7, 9, 12, 7, and 9 predominant components were identified in dorsal meat, belly meat, red meat, fish skin, fish intestines, and fish gills. The fishy smell detected from strongest to weakest were: fish gills, fish intestines, fish skin, red meat, and dorsal meat/belly meat. Practical applications Freshwater fish generally have an unattractive fishy, earthy odor thus not favored by consumers. The present study reveals volatile components present in different parts of grass carp using monolithic material sorptive extraction gas chromatography–mass spectrometry (MMSE–GC–MS) and electronic nose. The results demonstrated that combination of electronic nose response, principle component analysis, and MMSE–GC–MS can be applied to distinguish the flavors of the red meat, fish skin, fish intestines and fish gills, the dorsal meat and belly meat successfully and this method could be used for the identification of different compounds in similar gas chromatography–mass spectrometry analysis. The findings of this study will enrich our theoretical knowledge of flavor chemistry of fish products.

show abstract

“…A design similar to the SBSE/Twister W concept led to the development of Monolithic Material Sorptive Extraction (MonoTrap TM ) based on Merck DGaA monolithic technology (GL Science Inc., Tokyo, Japan). The monolithic-coated bars can be used for sampling liquids and volatile compounds by HS, prior to solvent desorption and GC analysis [28,29].…”

Section: Introductionmentioning

confidence: 99%

Solid phase microextraction techniques used for gas chromatography: a review

Dugheri

Mucci

Bonari

et al. 2020

Acta Chromatographica

View full text Add to dashboard Cite

In the last decade, the development and adoption of greener and sustainable microextraction techniques have been proved to be an effective alternative to classical sample preparation procedures. In this review, 10 commercially available solid-phase microextraction systems are presented, with special attention to the appraisal of their analytical, bioanalytical, and environmental engineering. This review provides an overview of the challenges and achievements in the application of fully automated miniaturized sample preparation methods in analytical laboratories. Both theoretical and practical aspects of these environment-friendly preparation approaches are discussed. The application of chemometrics in method development is also discussed. We are convinced that green analytical chemistry will be really useful in the years ahead. The application of cheap, fast, automated, “clever”, and environmentally safe procedures to environmental, clinical, and food analysis will improve significantly the quality of the analytical data.

show abstract

Optimization of Disk Sorptive Extraction Based on Monolithic Material for the Determination of Aroma Compounds from Lantana camara L. by Gas Chromatography-Mass Spectrometry

Cited by 14 publications

References 14 publications

Elucidation of Biochemical Pathways Underlying VOCs Production in A549 Cells

Elucidation of Biochemical Pathways Underlying VOCs Production in A549 Cells

Volatile components present in different parts of grass carp

Solid phase microextraction techniques used for gas chromatography: a review

Contact Info

Product

Resources

About