Optimization of direct shoot regeneration using cotyledonary explants and true leaves from lettuce cv. Romaine (Lactuca sativa L.) by surface response methodology

Gómez-Montes, Elías Octavio; Oliver-Salvador, Carmen; Durán-Figueroa, Noé; Badillo-Corona, Jesús Agustín; Salas, Carlos

doi:10.1007/s10725-015-0067-5

Cited by 11 publications

(2 citation statements)

References 31 publications

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“…Using DoE, correct nutrient combinations were identified, along with the effects of their interactions with the other environmental parameters: plant growth regulators. Similar methods have recently been used for optimizing callus cultures of various plant species [ 35 , 37 , 38 ]. This method has two advantages over the more common factorial design: DoE allows us to investigate the effects of independent variables between the actual experimental data points and allows a researcher to easily increase the number of experimental variables to more than five, which is not practical in typical factorial designs.…”

Section: Discussionmentioning

confidence: 99%

Optimization of Biomass Accumulation and Production of Phenolic Compounds in Callus Cultures of Rhodiola rosea L. Using Design of Experiments

Эрст

Petruk

Erst

et al. 2022

Plants

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Rhodiola rosea L. is a valuable medicinal plant with adaptogenic, neuroprotective, antitumor, cardioprotective, and antidepressant effects. In this study, design of experiments methodology was employed to analyze and optimize the interacting effects of mineral compounds (concentration of NO3− and the ratio of NH4+ to K+) and two plant growth regulators [total 6-benzylaminopurine (BAP) and α-naphthylacetic acid (NAA) concentration and the ratio of BAP to NAA] on the growth and the production of total phenolic compounds (TPCs) in R. rosea calluses. The overall effect of the model was highly significant (p < 0.0001), indicating that NH4+, K+, NO3−, BAP, and NAA significantly affected growth. The best callus growth (703%) and the highest production of TPCs (75.17 mg/g) were achieved at an NH4+/K+ ratio of 0.33 and BAP/NAA of 0.33, provided that the concentration of plant growth regulators was 30 μM and that of NO3− was ≤40 mM. According to high-performance liquid chromatography analyses of aerial parts (leaves and stems), in vitro seedlings and callus cultures of R. rosea contain no detectable rosarin, rosavin, rosin, and cinnamyl alcohol. This is the first report on the creation of an experiment for the significant improvement of biomass accumulation and TPC production in callus cultures of R. rosea.

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Section: Discussionmentioning

confidence: 99%

Optimization of Biomass Accumulation and Production of Phenolic Compounds in Callus Cultures of Rhodiola rosea L. Using Design of Experiments

Эрст

Petruk

Erst

et al. 2022

Plants

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“…Regeneration normally proceeds by organogenesis, but SE has also been reported. Recently, the “surface response” method has been employed in lettuce to optimize plant regeneration ( Gómez-Montes et al, 2015 ). While a mature regeneration/transformation technology is available for both above-mentioned species, de novo shoot regeneration and cell transformation have been observed only sporadically and with very low efficiency in another important vegetable of this family, i.e., globe artichoke ( Cynara cardunculus var.…”

Section: Regeneration and Transformationmentioning

confidence: 99%

Genetic Transformation and Genomic Resources for Next-Generation Precise Genome Engineering in Vegetable Crops

2017

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In the frame of modern agriculture facing the predicted increase of population and general environmental changes, the securement of high quality food remains a major challenge to deal with. Vegetable crops include a large number of species, characterized by multiple geographical origins, large genetic variability and diverse reproductive features. Due to their nutritional value, they have an important place in human diet. In recent years, many crop genomes have been sequenced permitting the identification of genes and superior alleles associated with desirable traits. Furthermore, innovative biotechnological approaches allow to take a step forward towards the development of new improved cultivars harboring precise genome modifications. Sequence-based knowledge coupled with advanced biotechnologies is supporting the widespread application of new plant breeding techniques to enhance the success in modification and transfer of useful alleles into target varieties. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 system, zinc-finger nucleases, and transcription activator-like effector nucleases represent the main methods available for plant genome engineering through targeted modifications. Such technologies, however, require efficient transformation protocols as well as extensive genomic resources and accurate knowledge before they can be efficiently exploited in practical breeding programs. In this review, we revise the state of the art in relation to availability of such scientific and technological resources in various groups of vegetables, describe genome editing results obtained so far and discuss the implications for future applications.

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Optimization of shikonin production in Onosma dichroantha callus using response surface methodology

Bagherieh‐Najjar

Nezamdoost

2016

Plant Cell Tiss Organ Cult

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Optimization of direct shoot regeneration using cotyledonary explants and true leaves from lettuce cv. Romaine (Lactuca sativa L.) by surface response methodology

Cited by 11 publications

References 31 publications

Optimization of Biomass Accumulation and Production of Phenolic Compounds in Callus Cultures of Rhodiola rosea L. Using Design of Experiments

Optimization of Biomass Accumulation and Production of Phenolic Compounds in Callus Cultures of Rhodiola rosea L. Using Design of Experiments

Genetic Transformation and Genomic Resources for Next-Generation Precise Genome Engineering in Vegetable Crops

Optimization of shikonin production in Onosma dichroantha callus using response surface methodology

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