Optimization of detergents in solubilization and reconstitution of Aquaporin Z: A structural approach

Beratto-Ramos, Angelo; Dagnino‐Leone, Jorge; Martínez‐Oyanedel, José; Fernández, Marcos; Aranda, Mario; Bórquez, Rodrigo

doi:10.1016/j.bbamem.2022.184101

Cited by 4 publications

(1 citation statement)

References 55 publications

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“…The surface roughness was also analyzed using AFM after each step. The decrease in the surface roughness of the AQP membrane indicates the coating quality or uniform coating layer of proteoliposomes on the substrate surface, which ensures the functionality of the aquaporin protein after coating 7,28,29 . The formation of liposome/proteoliposome aggregates (which may affect the structure and performance of the aquaporin protein) on the surface during coating indicates the formation of valleys, which consequently increases the surface roughness and thus decreases the performance of the AQP membrane.…”

Section: Aqp Aao Membrane Fabricationmentioning

confidence: 99%

Highly Permeable and Shelf-Stable Aquaporin Biomimetic Membrane Based on an Anodic Aluminum Oxide Substrate

Fuwad

Ryu

Han

et al. 2023

Preprint

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Section: Aqp Aao Membrane Fabricationmentioning

confidence: 99%

Highly Permeable and Shelf-Stable Aquaporin Biomimetic Membrane Based on an Anodic Aluminum Oxide Substrate

Fuwad

Ryu

Han

et al. 2023

Preprint

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Mechanisms of Triton X-100 reducing the Ag+-resistance of Enterococcus faecalis

Lv,

Duan,

Fan

et al. 2024

World J Microbiol Biotechnol

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Highly permeable and shelf-stable aquaporin biomimetic membrane based on an anodic aluminum oxide substrate

Fuwad,

Ryu,

Han

et al. 2024

npj Clean Water

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Aquaporin (AQP) biomimetic membranes are a coming-of-age technology for water purification. Although several studies have reported aquaporin biomimetic membrane fabrication to date, these membranes show low water flux mainly due to the low porosity and inherently dense structure of the polymeric substrate materials. Herein, we report a ceramic-based aquaporin biomimetic membrane based on anodic aluminum oxide (AAO) as a substrate, which has a uniform porous structure with a high aspect ratio and pore density compared to conventional polymer substrates and exhibits a high water flux of 27.6 ± 3.6 LMH (L m−2 h−1) and superior membrane selectivity of 0.11 g L−1. Briefly, the AAO substrate was functionalized with amino-silane followed by polydopamine coating, then the AQP vesicles were immobilized on the functionalized AAO substrate surface using an electrokinetic method, and the water rejection performance of the membrane was analyzed in a forward osmosis system. Furthermore, a simple cryodesiccation method is introduced to improve the storage stability and easy transportation of aquaporin membranes, which does not require special environmental conditions to transport or store them.

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Optimization of detergents in solubilization and reconstitution of Aquaporin Z: A structural approach

Cited by 4 publications

References 55 publications

Highly Permeable and Shelf-Stable Aquaporin Biomimetic Membrane Based on an Anodic Aluminum Oxide Substrate

Highly Permeable and Shelf-Stable Aquaporin Biomimetic Membrane Based on an Anodic Aluminum Oxide Substrate

Mechanisms of Triton X-100 reducing the Ag+-resistance of Enterococcus faecalis

Highly permeable and shelf-stable aquaporin biomimetic membrane based on an anodic aluminum oxide substrate

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