Optimization of Culture Medium for <i>Lactobacillus bulgaricus</i> using Box-Behnken Design

Zhang, Bowen; Shu, Guowei; Bao, Chunju; Cao, Jili; Tan, Yuan

doi:10.1515/aucft-2017-0001

Cited by 9 publications

(4 citation statements)

References 10 publications

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“…LAB has a limited capacity to synthesize amino acids and is therefore dependent on the use of exogenous nitrogen sources for optimal growth (Hebert et al, 2000; proteinase produced by Lactobacillus plantarum LP69 Espeche et al, 2009). Chen et al (2016) reported that glutamate, soybean peptone and casein hydrolysate can improve the viable cells number of Streptococcus thermophilus. There is a significant effect on Soya peptone to the growth of S. thermophilus and the optimum soya peptone content was 30g/L .…”

Section: Effect Of Nitrogen Source On L Plantarum Lp69 Producing Cepmentioning

confidence: 99%

Effects of Carbon and Nitrogen Sources on Activity of Cell Envelope Proteinase Produced by Lactobacillus plantarum LP69

Cheng

Chen

et al. 2019

Acta Universitatis Cibiniensis. Series E: Food Technology

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In present study, the effects of carbon sources (glucose, lactose, sucrose, galactose, maltose and soluble starch) and nitrogen sources (casein peptone, whey protein, soy peptone, yeast, tryptone, beef extract and peptone) on activity of cell envelope proteinases (CEP), specific activity, protein content, OD600 value and pH in MRS broth fermented by Lactobacillus plantarum LP69 were investigated by individual factor experiment. The results indicated that carbon and nitrogen sources have significant influence on the activity of CEP and specific activitys of L. plantarum LP69, glucose, maltose, casein peptone and peptone are superior to other selected carbon and nitrogen sources. The optimum concentrations of glucose, maltose, casein peptone and peptone for L. plantarum LP69 are 2%, 2%, 1% and 1%; the activity of CEP are 19.52U/mL, 21.13U/mL, 13.49U/mL and 20.61U/mL, respectively.

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Section: Effect Of Nitrogen Source On L Plantarum Lp69 Producing Cepmentioning

confidence: 99%

Effects of Carbon and Nitrogen Sources on Activity of Cell Envelope Proteinase Produced by Lactobacillus plantarum LP69

Cheng

Chen

et al. 2019

Acta Universitatis Cibiniensis. Series E: Food Technology

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“…It is well known that many factors affect enzymatic activity in fruits and vegetables and which can be reducing the number of required essays (Yim et al, 2012). The RSM is widely used to optimize synthesis of bioactive molecules, extraction of desired compounds, and activity of enzymes (Yamaguchi et al, 2006;Zhang et al, 2017;Pramana et al, 2018). To the best of our knowledge, this work is the first study concerning the enzymatic activity optimization of the peroxidase extracted from the turnip.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Peroxidase Activity of Turnip (Brassica rapa) Using Response Surface Methodology

Dahdouh

Bachir‐bey

Katı

2020

Acta Universitatis Cibiniensis. Series E: Food Technology

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The present study is focused on the determination of optimized activity conditions of peroxidase (POD) of turnip (Brassica rapa). The enzyme is extracted from turnip with 0.5M Na2HPO4 (pH7.0). Peroxidase activity is evaluated by monitoring the oxidation of guaiacol to tetraguaiacol in the presence of hydrogen peroxide (H2O2). Before the optimization of peroxidase activity from a turnip by response surface methodology (RSM), a preliminary step is conducted for each studied parameter (pH, temperature, and NaCl concentration). Further, RSM following Box-Behnken design is used in order to refine the optimization of the conditions. Response surface analysis showed that parameter values with the highest peroxidase activity are 5.9, 29.8°C, and 1.07M for pH, temperature, and NaCl, respectively. Under these experimental conditions, peroxidase activity is found to be 4027.25U/mg. The obtained experimental value agrees with that of the calculated one, indicating the pertinence of the developed model for the optimization of peroxidase activity.

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“…Response surface methodology (RSM), also called the Box-Wilson methodology, is a useful tool to optimize the factors of fermentation processes by applying mathematics and statistics [23]. This technique is an experimental design based on the fit of a polynomial regression model for testing the multiple factors that influence the responses by varying the factors simultaneously, and fewer experiments are needed to study the effects of all factors [24,25]. Through RSM, the interaction effects of individual factors can also be determined.…”

Section: Introductionmentioning

confidence: 99%

Development of a low-cost culture medium for the rapid production of plant growth-promoting Rhodopseudomonas palustris strain PS3

Lee

Liu

2020

PLoS ONE

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Rhodopseudomonas palustris PS3 is one of the purple phototrophic non-sulfur bacteria (PNSB), which have plant growth-promoting effects on various plants. To expand the scale of PS3 fermentation in a time-and cost-effective fashion, the purpose of this work was to evaluate the use of low-cost materials as culture media and to optimize the culture conditions via response surface methodology. Corn steep liquor (CSL) and molasses were identified as potential materials to replace the nitrogen and carbon sources, respectively, in the conventional growth medium. The optimum culture conditions identified through central composite design were CSL, 39.41 mL/L; molasses, 32.35 g/L; temperature, 37.9˚C; pH, 7.0; and DO 30%. Under the optimized conditions, the biomass yield reached 2.18 ± 0.01 g/ L at 24 hours, which was 7.8-fold higher than that under the original medium (0.28 ± 0.01 g/ L). The correlation between the predicted and experimental values of the model was over 98%, which verified the validity of the response models. Furthermore, we verified the effectiveness of the R. palustris PS3 inoculant grown under the newly developed culture conditions for plant growth promotion. This study provides a potential strategy for improving the fermentation of R. palustris PS3 in low-cost media for large-scale industrial production.

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Optimization of Culture Medium for Lactobacillus bulgaricus using Box-Behnken Design

Cited by 9 publications

References 10 publications

Effects of Carbon and Nitrogen Sources on Activity of Cell Envelope Proteinase Produced by Lactobacillus plantarum LP69

Effects of Carbon and Nitrogen Sources on Activity of Cell Envelope Proteinase Produced by Lactobacillus plantarum LP69

Optimization of Peroxidase Activity of Turnip (Brassica rapa) Using Response Surface Methodology

Development of a low-cost culture medium for the rapid production of plant growth-promoting Rhodopseudomonas palustris strain PS3

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