Optimization of culture medium components and culture period for production of adventitious roots of Echinacea pallida (Nutt.) Nutt

Gao, Yuan; Wu, Chunhua; Piao, Xuan Chun; Han, Lu; Gao, Ri; Lian, Mei-Lan

doi:10.1007/s11240-018-1464-z

Cited by 11 publications

(2 citation statements)

References 26 publications

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“…Le et al (2019) reported that application of available concentrations of sucrose in culture medium can significantly promote AR growth [ 64 ]. At the same time, sucrose not only affects AR growth, but also influences the substance metabolism of ARs in Echinacea pallida [ 65 ]. Takahashi et al (2003) showed that the role of sucrose on AR formation is similar to that of hormones and that the induction stage of AR development is strictly controlled by sucrose [ 66 ].…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiling reveals the effects of light on adventitious root formation in lotus seedlings (Nelumbo nucifera Gaertn.)

Cheng¹,

Han²,

Minrong³

et al. 2020

BMC Genomics

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Background Lotus is an aquatic horticultural crop that is widely cultivated in most regions of China and is used as an important off-season vegetable. The principal root of lotus is degenerated, and adventitious roots (ARs) are irreplaceable for plant growth. We found that no ARs formed under darkness and that exposure to high-intensity light significantly promoted the development of root primordia. Four differential expression libraries based on three light intensities were constructed to monitor metabolic changes, especially in indole-3-acetic acid (IAA) and sugar metabolism. Results AR formation was significantly affected by light, and high light intensity accelerated AR development. Metabolic changes during AR formation under different light intensities were evaluated using gene expression profiling by high-throughput tag-sequencing. More than 2.2 × 104 genes were obtained in each library; the expression level of most genes was between 0.01 and 100 (FPKF value). Libraries constructed from plants grown under darkness (D/CK), under 5000 lx (E/CK), and under 20,000 lx (F/CK) contained 1739, 1683, and 1462 upregulated genes and 1533, 995, and 834 downregulated genes, respectively, when compared to those in the initial state (CK). Additionally, we found that 1454 and 478 genes had altered expression in a comparison of libraries D/CK and F/CK. Gene transcription between libraries D/F ranged from a 5-fold decrease to a 5-fold increase. Twenty differentially expressed genes (DEGs) were involved in the signal transduction pathway, 28 DEGs were related to the IAA response, and 35 DEGs were involved in sugar metabolism. We observed that the IAA content was enhanced after seed germination, even in darkness; this was responsible for AR formation. We also observed that sucrose could eliminate the negative effect of 150 μMol IAA during AR development. Conclusions AR formation was regulated by IAA, even in the dark, where induction and developmental processes could also be completed. In addition, 36 genes displayed altered expression in carbohydrate metabolism and ucrose metabolism was involved in AR development (expressed stage) according to gene expression and content change characteristics.

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Section: Discussionmentioning

confidence: 99%

Gene expression profiling reveals the effects of light on adventitious root formation in lotus seedlings (Nelumbo nucifera Gaertn.)

Cheng¹,

Han²,

Minrong³

et al. 2020

BMC Genomics

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show abstract

“…Le et al (2019) report that application of available concentration of sucrose in culture medium can significantly promote ARs growth [65]. At the same time, sucrose not only affects ARs growth, but has influence on substance metabolism of ARs in Echinacea pallida [66]. Takahashi et al (2003) show that the role of sucrose on ARs formation is similar with hormone, and induction stage of ARs development is strictly controlled by sucrose [67].…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiling reveals light regulation on adventitious root formation in lotus (Nelumbo nucifera Gaertn.)

Cheng¹,

yuyan²,

minrong³

et al. 2020

Preprint

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Background: Lotus is an aquatic horticultural crop, and widely cultivated in most regions of China.Lotus is often used as a kind of an important off-season vegetable with various nutrients. Principle root of lotus is degenerated, and the role of adventitious roots (ARs) is Irreplaceable for plant growth.We found that no ARs could be formed under darkness condition, and high light significantly promote the development of root primordium. Therefore, four libraries with three light intensities were constructed to monitor metabolism changes, especially in IAA and sugar metabolism.Results: We found that ARs formation was significantly affected by light, high light intensity accelerated ARs development. The change of metabolism during ARs formation under different light intensity was evaluated according to gene expression profiling by high-throughput tag-sequencing. It was shown that more than 2.2× 10 4 genes was obtained in each library, and the expression level of most genes were distributed between 1e-01 and 1e+03 (FPKF value). Among these identified genes, 1739, 1683 and 1462 genes were up-regulated, and 1533, 995 and 834 genes were down-regulated in D/CK, E/CK and F/CK libraries respectively. In addition, we also found that 1454 and 478 genes changed expression in D/CK and F/CK libraries when compared D/CK with F/CK libraries. In F/D libraries, the transcriptional level of most differentially expressed genes was between -5~5 fold, and twenty differentially expressed genes were involved in signal transduction pathway. Twenty-eight genes related with IAA response and thirty-five genes involved in sugar metabolism were found to change expression. It was elucidated that IAA content was enhanced after seed germinated, even in darkness condition, which was responsible for ARs formation.Conclusion: The process of ARs formation was very complex, and regulated by multiple factors. The ARs formation was regulated by IAA, even in the dark, induction and developmental process could also be completed. In addition, the genes (36 genes) changed expression level in carbohydrate metabolism showed the third number, so sucrose metabolism was involved in the ARs development (expressed stage) according to genes expression and content change characteristic.

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Biotechnological Production of Useful Phytochemicals from Adventitious Root Cultures

Murthy¹,

Dalawai²,

Bhat³

et al. 2019

Reference Series in Phytochemistry

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Optimization of culture medium components and culture period for production of adventitious roots of Echinacea pallida (Nutt.) Nutt

Cited by 11 publications

References 26 publications

Gene expression profiling reveals the effects of light on adventitious root formation in lotus seedlings (Nelumbo nucifera Gaertn.)

Gene expression profiling reveals the effects of light on adventitious root formation in lotus seedlings (Nelumbo nucifera Gaertn.)

Gene expression profiling reveals light regulation on adventitious root formation in lotus (Nelumbo nucifera Gaertn.)

Biotechnological Production of Useful Phytochemicals from Adventitious Root Cultures

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