Optimization of Culture Conditions for the Production of Biosurfactants

Sharma, Swati; Tiwari, Pankaj; Pandey, Lalit M.

doi:10.1007/978-981-16-5465-7_7

Cited by 2 publications

(2 citation statements)

References 77 publications

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“…Supplementation of carbon sources in medium is an essential for enhancement of BS productivity (Sharma et al, 2022). The different carbon sources supported the BS synthesis (Figure 5a), but xylose was best for maximum, 2.03 ± 0.1 g/L production as compared to sucrose (1.63 ± 0.1 g/L) and lactose (1.54 ± 0.15 g/L).…”

Section: Resultsmentioning

confidence: 99%

Bioprocess optimization for glycopeptide biosurfactant production by means of Lactobacillus delbrueckii : Design expert laden approach

Goyal

Singh²

2022

Food Processing Preservation

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A bacterial strain, Lactobacillus delbrueckii, was isolated and screened for high potential of biosurfactant (BS) production yield. The current research emphasizes the optimization of process parameters through OFAT (one factor at a time) and RSM (response surface methodology) to achieve optimal BS production. Therefore, xylose and peptone were screened as the best carbon and nitrogen sources respectively by means of OFAT, along with temperature (37°C), inoculum size (5%), and fermentation time (39 h). Furthermore, optimization of xylose (1% w/v), peptone (0.5%w/v), and pH (6.5) through Box–Behnken statistical design was validated by ANOVA (analysis of variance) and found significant (p < .05) with optimal BS yield (7.31 g/L). The solvent‐extracted glycopeptide BS displayed cationic nature, with thermal (0–120°C), pH (2–12), and NaCl (2%–12% w/v) stability. The functional attributes of BS like antiadhesive and antimicrobial activity toward Gram‐positive and Gram‐negative bacteria and emulsifying potency against various vegetable oils make it worthwhile and applicable. Practical applications The recent applications of BS and hazards of pathogenic biofilm make it challenging to produce enhanced yield of BS. In light of this, present work is focused to optimize yield from Lactobacillus delbrueckii for its applications as antibiofilm and bioemulsifier agent in various industries. Furthermore, the glycoproteinaceous BS from microbe in question is itself unique and novel. Additionally, the statistical design of optimization provides experiment modeling, production rate, and significance of opted design which makes the whole process feasible to explore new possibilities for BS production.

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Section: Resultsmentioning

confidence: 99%

Bioprocess optimization for glycopeptide biosurfactant production by means of Lactobacillus delbrueckii : Design expert laden approach

Goyal

Singh²

2022

Food Processing Preservation

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“…Different essential liquid growth media, including defined and undefined broths, were selected to reflect a variety of nutrition sources. We used nine different medium such as Malt extract broth, Minimal Davis broth with Dextrose, Potato carrot broth, Potato Dextrose broth, Sabraoud's dextrose broth, Czapek Dox broth, Yeast Sucrose broth, Wickerham's broth and Tryptone broth (Sharma & Sharma, 2021) to determine the ideal medium for secondary metabolite production. The carbon and nitrogen sources combination in each medium were different.…”

Section: Optimization Of Fermentation Medium For the Secondary Metabo...mentioning

confidence: 99%

Optimization of culture conditions for the production, antifungal activity and characterization of secondary metabolites of <i>Trichoderma longibrachiatum</i>

Priya,

Balachander,

Prabhakaran

2023

JBC

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The present study was intended to optimize the culture conditions for secondary metabolite production by endophytic fungi. Based on the morphology and phylogeny, the fungus was identified as Trichoderma longibrachiatum isolated from brinjal leaf based on morphological characterization. The antifungal activity was evaluated against phytopathogens such as Macrophomina phaseolina, Phytopthora infestans, Colletotrichum falcatum and Colletotrichum gloeosporioides through the overlapping method, culture filtrate and organic fraction from Potato dextrose both as a growth medium. The organic fraction exhibited a significant antifungal activity, while modifications in medium composition may possess a major impact on the quantity and quality of secondary metabolites production. To achieve maximum metabolite production, the growth of the culture was optimized with screening of basal media, carbon, nitrogen, pH, trace elements and incubation period. The final optimized fermentation conditions were Minimal ereavis broth as basal media; glucose and sucrose as carbon source; Peptone and Yeast extract as nitrogen source, sodium nitrate as precursor; pH as 6; and incubation period as 7 days at 28°C. This optimization resulted in antifungal activity of 47.19-60.67% against M. phaseolina, P. infestans, C. falcatum, and C. gloeosporioides which was higher than that before optimization (43.80%). GCMS revealed distinct metabolites of T. longibrachiatum, comprising antifungal metabolites and molecules with additional bioactivities. These results strengthen ongoing research on disease control in agriculture by emphasizing the biocontrol potential of T. longibrachiatum isolated from brinjal phyllosphere against plant pathogenic fungi.

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Optimization of Culture Conditions for the Production of Biosurfactants

Cited by 2 publications

References 77 publications

Bioprocess optimization for glycopeptide biosurfactant production by means of Lactobacillus delbrueckii : Design expert laden approach

Bioprocess optimization for glycopeptide biosurfactant production by means of Lactobacillus delbrueckii : Design expert laden approach

Optimization of culture conditions for the production, antifungal activity and characterization of secondary metabolites of <i>Trichoderma longibrachiatum</i>

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