Optimization of Culture Conditions for Enhanced Antimicrobial Activity of Rhodococcus erythropolis VLK-12 Isolated from South Coast of Andhra Pradesh, India

Naragani, Krishna

doi:10.9734/bmrj/2014/5707

Cited by 8 publications

(11 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A previous report demonstrated high antimicrobial activity of crude extracts of a five-day-old culture of Rhodococcus erythropolis VL-RK_05. 29 Naragani et al 30 reported the presence of antimicrobial compounds in five-day-old culture extracts of Streptomyces violaceoruber VLK-4.…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial profile of Arthrobacter kerguelensis VL-RK_09 isolated from Mango orchards

Munaganti

Vijayalakshmi

Konda

et al. 2016

Brazilian Journal of Microbiology

Self Cite

View full text Add to dashboard Cite

An actinobacterial strain VL-RK_09 having potential antimicrobial activities was isolated from a mango orchard in Krishna District, Andhra Pradesh (India) and was identified as Arthrobacter kerguelensis. The strain A. kerguelensis VL-RK_09 exhibited a broad spectrum of in vitro antimicrobial activity against bacteria and fungi. Production of bioactive metabolites by the strain was the highest in modified yeast extract malt extract dextrose broth, as compared to other media tested. Lactose (1%) and peptone (0.5%) were found to be the most suitable carbon and nitrogen sources, respectively, for the optimum production of the bioactive metabolites. The maximum production of the bioactive metabolites was detected in the culture medium with an initial pH of 7, in which the strain was incubated for five days at 30 °C under shaking conditions. Screening of secondary metabolites obtained from the culture broth led to the isolation of a compound active against a wide variety of Gram-positive and negative bacteria and fungi. The structure of the first active fraction was elucidated using Fourier transform infrared spectroscopy, electrospray ionization mass spectrometry, 1H and 13C nuclear magnetic resonance spectroscopy. The compound was identified as S,S-dipropyl carbonodithioate. This study is the first report of the occurrence of this compound in the genus Arthrobacter.

show abstract

Section: Resultsmentioning

confidence: 99%

Antimicrobial profile of Arthrobacter kerguelensis VL-RK_09 isolated from Mango orchards

Munaganti

Vijayalakshmi

Konda

et al. 2016

Brazilian Journal of Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…This fungus shows antimicrobial activity against bacteria Bacillus subtilis ATCC 6633, S. aureus ATCC 25923, E. coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 by agar well diffusion method [37]. The antimicrobial activity of mangrove endophytic fungi is well-documented [7,9,[38][39][40]. The antimicrobial activity of Bacillus species was found maximally against K. oxytoca and V. cholerae, and minimally against S. paratyphi and S. aureus.…”

Section: Discussionmentioning

confidence: 99%

“…However, thraustochytrids are rarely attempted for their potential for antimicrobials [4][5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Polyunsaturated fatty acid-producing marine thraustochytrids: A potential source for antimicrobials

2015

J Coast Life Med

View full text Add to dashboard Cite

“…The optimum pH for antibiotic production by several actinomycetes was reported to be 7 for Streptomyces hygroscopicus D1.5 [28], Streptomyces torulosus KH-4 [29], Streptomyces sp. VITSVK 9 [30], Streptomyces cellulosae VJDS-1 [31], Rhodococcus erythropolis VLK-12 [32], and A. kerguelensis VL-RK_09 [18].…”

Section: Impact Of Ph and Temperature On Antimicrobial Activitymentioning

confidence: 99%

Optimization, Isolation and Characterization of Bioactive Compounds From Streptomyces Lavendulocolor VHB-9

Bssn

Munaganti

Vijayalakshmi

et al. 2018

Asian J Pharm Clin Res

Self Cite

View full text Add to dashboard Cite

Objectives: Optimization, isolation, and characterization of bioactive compounds from Streptomyces lavendulocolor VHB-9 isolated from granite mines of Mudigonda village of Khammam district of Telangana state. Methods:The potent strain was identified as S. lavendulocolor VHB-9 by polyphasic taxonomy. The influence of culture conditions on growth and bioactive compounds production was investigated. Purification of bioactive compounds was done using column chromatography. The structures of the compounds were elucidated on the basis of spectroscopic analysis including Fourier transform infrared, electron spray ionization mass spectrophotometry, 1 H nuclear magnetic resonance (NMR), and 13 C NMR. The antimicrobial activity of the compounds produced by the strain was tested against both Gram-positive and Gram-negative bacteria and fungi in terms of minimum inhibitory concentration.Results: Isolation and identification of two compounds, namely (2R, 3R)-2, 3-Butanediol (B1A), and nonadecanoic acid (B1B). Fraction B4 was isolated partially purified fraction and identified by the gas chromatography-mass spectrometry analysis. B1B compound exhibited the highest activity against Bacillus megaterium, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans when compared to B1A and B4 compounds.

show abstract

Optimization of Culture Conditions for Enhanced Antimicrobial Activity of Rhodococcus erythropolis VLK-12 Isolated from South Coast of Andhra Pradesh, India

Cited by 8 publications

References 15 publications

Antimicrobial profile of Arthrobacter kerguelensis VL-RK_09 isolated from Mango orchards

Antimicrobial profile of Arthrobacter kerguelensis VL-RK_09 isolated from Mango orchards

Polyunsaturated fatty acid-producing marine thraustochytrids: A potential source for antimicrobials

Optimization, Isolation and Characterization of Bioactive Compounds From Streptomyces Lavendulocolor VHB-9

Contact Info

Product

Resources

About