Optimization of culture conditions for violacein production by a new strain of Duganella sp. B2

Wang, Haisheng; Jiang, Pei; Lu, Yuan; Ruan, Zhiyong; Rui-bo, Jiang; Xing, Xin‐Hui; Lou, Kai; Wei, Dong

doi:10.1016/j.bej.2008.11.008

Cited by 68 publications

(52 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Violacein is produced by three bacterial species in the betaproteobacterial group closely related to J. lividum: Duganella sp. (19), Chromobacterium violaceum, and Iodobacter fluviatile (7,12). In addition, Pseudoalteromonas tunicata and Pseudoalteromonas luteoviolacea in the gammaproteobacterial group produce violacein, which protects P. tunicata from protozoan grazing and may protect its hosts, such as algae and tunicates, from fouling (13,15,23).…”

Section: Discussionmentioning

confidence: 99%

The Bacterially Produced Metabolite Violacein Is Associated with Survival of Amphibians Infected with a Lethal Fungus

Becker

Brucker

Schwantes

et al. 2009

Appl Environ Microbiol

183

161

View full text Add to dashboard Cite

The disease chytridiomycosis, which is caused by the chytrid fungus Batrachochytrium dendrobatidis, is associated with recent declines in amphibian populations. Susceptibility to this disease varies among amphibian populations and species, and resistance appears to be attributable in part to the presence of antifungal microbial species associated with the skin of amphibians. The betaproteobacterium Janthinobacterium lividum has been isolated from the skins of several amphibian species and produces the antifungal metabolite violacein, which inhibits B. dendrobatidis. In this study, we added J. lividum to red-backed salamanders (Plethodon cinereus) to obtain an increased range of violacein concentrations on the skin. Adding J. lividum to the skin of the salamander increased the concentration of violacein on the skin, which was strongly associated with survival after experimental exposure to B. dendrobatidis. As expected from previous work, some individuals that did not receive J. lividum and were exposed to B. dendrobatidis survived. These individuals had concentrations of bacterially produced violacein on their skins that were predicted to kill B. dendrobatidis. Our study suggests that a threshold violacein concentration of about 18 M on a salamander's skin prevents mortality and morbidity caused by B. dendrobatidis. In addition, we show that over one-half of individuals in nature support antifungal bacteria that produce violacein, which suggests that there is a mutualism between violaceinproducing bacteria and P. cinereus and that adding J. lividum is effective for protecting individuals that lack violacein-producing skin bacteria.The amphibian fungal pathogen Batrachochytrium dendrobatidis causes a lethal skin disease that has caused substantial declines in amphibian populations (18). However, some species, such as the bullfrog (Rana catesbeiana) and the tiger salamander (Ambystoma tigrinum), are relatively asymptomatic when they are infected with this pathogen (4, 5). Variation in survival among species has been attributed to differences in innate immune factors, such as antimicrobial peptides (20) and skin-associated microbial species (8-11), as well as behavior (16). The presence of antifungal microbes is of particular interest because it suggests that these organisms are mutualistic associates of amphibian species. In addition, augmentation of the cutaneous microbial community by adding species of bacteria that inhibit B. dendrobatidis has the potential to provide resistance to chytridiomycosis (9).We have identified a number of bacteria associated with the skin of amphibians that inhibit B. dendrobatidis in vitro via secretion of antifungal metabolites (2,3,10,11). The bacterial species used in this study, Janthinobacterium lividum, produces the anti-B. dendrobatidis metabolites violacein and indole-3-carboxaldehyde (MIC, 1.82 M and 69 M, respectively) (3). We have shown that violacein inhibits B. dendrobatidis in laboratory assays (3) and is strongly correlated with survival in vivo of the frog species Ra...

show abstract

Section: Discussionmentioning

confidence: 99%

The Bacterially Produced Metabolite Violacein Is Associated with Survival of Amphibians Infected with a Lethal Fungus

Becker

Brucker

Schwantes

et al. 2009

Appl Environ Microbiol

183

161

View full text Add to dashboard Cite

show abstract

“…Recently, the stable and efficient production of crude Vio (at ≥1.6 gL −1 ) in recombinant Citrobacter freundii has been successfully achieved by expressing vioabcde from Duganella sp. B2 by our group by using glycerol as carbon source which was reported to stimulate Vio production (Wang et al 2009;Jiang et al 2010). C. freundii may be a suitable host for the efficient synthesis of violacein and its derivatives own to the presence of multiple resistance genes and class 1 integrons in C. freundii (Ahmed and Shimamoto 2008;Srinivasan et al 2008), which might play an important role in resisting the antimicrobial activity of metabolites such as violacein, thereby leading to the high productivity.…”

Section: Introductionmentioning

confidence: 97%

Pathway redesign for deoxyviolacein biosynthesis in Citrobacter freundii and characterization of this pigment

Jiang

Wang

Fang

et al. 2012

Appl Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

Violacein (Vio) is an important purple pigment with many potential bioactivities. Deoxyviolacein, a structural analog of Vio, is always synthesized in low concentrations with Vio in wild-type bacteria. Due to deoxyviolacein's low production and difficulties in isolation and purification, little has been learned regarding its function and potential applications. This study was the first effort in developing a stable and efficient biosynthetic system for producing pure deoxyviolacein. A recombinant plasmid with vioabce genes was constructed by splicing using an overlapping extension-polymerase chain reaction, based on the Vio-synthesizing gene cluster of vioabcde, originating from Duganella sp. B2, and was introduced into Citrobacter freundii. With the viod gene disrupted in the Vio synthetic pathway, Vio production was completely abolished and the recombinant C. freundii synthesized only deoxyviolacein. Interestingly, vioe gene expression was strongly stimulated in the viod-deleted recombinant strain, indicating that viod disruptions could potentially induce polar effects upon the downstream vioe gene within this small operon. Deoxyviolacein production by this strain reached 1.9 g/L in shaker flasks. The product exhibited significant acid/alkali and UV resistance as well as significant inhibition of hepatocellular carcinoma cell proliferation at low concentrations of 0.1-1 μM. These physical characteristics and antitumor activities of deoxyviolacein contribute to illuminating its potential applications.

show abstract

“…Response surface methodology (RSM) is a practical statistical method for dealing with complex relationship and optimizing various factors. Medium composition has been optimized by RSM with Box-Behnken experimental design for production of valuable compounds (Wang et al, 2009). In this study, effect of wheat bran extract, l-tyrosine, and CuSD 4 on melanin yield was investigated.…”

Section: Introductionmentioning

confidence: 99%

Optimization of culture medium for production of melanin by Auricularia auricula

Zou

Hou

2017

Food Sci. Technol

View full text Add to dashboard Cite

Melanin is a natural high molecular weight pigment with the huge application value and development potential in food industry. In the present study, medium composition for melanin production by fungus Auricularia auricula was investigated. Wheat bran extract, l-tyrosine, and CuSD 4 were determined to optimize medium composition by response surface methodology with Box-Behnken design. Results indicated that the optimal medium composition was 26.80% (v/v) wheat bran extract, 1.59 g/L l-tyrosine, and 0.11 g/L CuSD 4 , and the maximum melanin yield was 519.54 mg/L. Melanin production through A. auricula fermentation avoided expensive enzymatic or complicated chemical methods for melanin extraction from tissues of plant or animal, which had the huge application value and development potential for efficient production of melanin.Keywords: optimization; culture medium; melanin; Auricularia auricula.Practical Application: Melanin production through A. auricula fermentation is a new preparation method of melanin.

show abstract

Optimization of culture conditions for violacein production by a new strain of Duganella sp. B2

Cited by 68 publications

References 22 publications

The Bacterially Produced Metabolite Violacein Is Associated with Survival of Amphibians Infected with a Lethal Fungus

The Bacterially Produced Metabolite Violacein Is Associated with Survival of Amphibians Infected with a Lethal Fungus

Pathway redesign for deoxyviolacein biosynthesis in Citrobacter freundii and characterization of this pigment

Optimization of culture medium for production of melanin by Auricularia auricula

Contact Info

Product

Resources

About