Optimization of Culture Conditions for Growth and Docosahexaenoic Acid Production by a Marine Thraustochytrid, Aurantiochytrium limacinum mh0186

Nagano, Naoki; Taoka, Yousuke; Honda, Daiske; Hayashi, Masahiro

doi:10.5650/jos.58.623

Cited by 62 publications

(25 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar studies have been performed on the DHA-producing fungal species Thraustochyrium aureum (Hur 2002, Ge 2011) and marine thraustochytrid A. limacinum (Nagano 2009) found quite different patterns from those that we have observed for M. marina. For example, Hur et al found that glucose supplementation had no impact on DHA content per biomass, while we found that DHA content per biomass decreased in the presence of glucose.…”

Section: Discussionsupporting

confidence: 69%

See 1 more Smart Citation

Growth condition optimization for docosahexaenoic acid (DHA) production by Moritella marina MP-1

Kautharapu

Rathmacher

Jarboe

2012

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

The marine organism Moritella marina MP-1 produces the polyunsaturated fatty acid docosahexaenoic acid (DHA). While the basic metabolic pathway for DHA production in this organism has been identified, the impact of growth conditions on DHA production is largely unknown. This study examines the effect of supplemental carbon, nitrogen and salts, growth temperature and media composition and pH on DHA and biomass production and the fatty acid profile. The addition of supplemental nitrogen significantly increased the overall DHA titer via an increase in biomass production. Supplemental glucose or glycerol increased biomass production, but decreased the amount of DHA per biomass, resulting in no net change in the DHA titer. Acidification of the baseline media pH to 6.0 increased DHA per biomass. Changes in growth temperature or provision of supplemental sodium or magnesium chloride did not increase DHA titer. This organism was also shown to grow on defined minimal media. For both media types, glycerol enabled more DHA production per biomass than glucose. Combination of these growth findings into marine broth supplemented with glycerol, yeast extract, and tryptone at pH 6.0 resulted in a final titer of 82 Â± 5 mg/L, a nearly eightfold increase relative to the titer of 11 Â± 1 mg/L seen in the unsupplemented marine broth. The relative distribution of other fatty acids was relatively robust to growth condition, but the presence of glycerol resulted in a significant increase in myristic acid (C14:0) and decrease in palmitic acid (C16:0). In summary, DHA production by M. marina MP-1 can be increased more than fivefold by changing the growth media. Metabolic engineering of this organism to increase the amount of DHA produced per biomass could result in additional increases in titer. ABSTRACTThe marine organism Moritella marina MP-1 produces the polyunsaturated fatty acid docosahexaenoic acid (DHA). While the basic metabolic pathway for DHA production in this organism has been identified, the impact of growth conditions on DHA production is largely unknown. This study examines the effect of supplemental carbon, nitrogen and salts, growth temperature and media composition and pH on DHA and biomass production and the fatty acid profile. The addition of supplemental nitrogen significantly increased the overall DHA titer via an increase in biomass production. Supplemental glucose or glycerol increased biomass production, but decreased the amount of DHA per biomass, resulting in no net change in the DHA titer.Acidification of the baseline media pH to 6.0 increased DHA per biomass. Changes in growth temperature or provision of supplemental sodium or magnesium chloride did not increase DHA titer. This organism was also shown to grow on defined minimal media. For both media types, glycerol enabled more DHA production per biomass than glucose. Combination of these growth findings into marine broth supplemented with glycerol, yeast extract and tryptone at pH 6.0 resulted in a final titer of 82+5 mg/L, a nearly 8-fold increase...

show abstract

Section: Discussionsupporting

confidence: 69%

“…This titer is 5-fold higher than the 13.7 mg/L achieved by supplementing M. marina culture with the antibiotic cerulenin (Morita 2005b), but drastically lower than the 4.3 g/L reported to be produced by the marine thraustochytrid Aurantiochyrium limacinum (Nagano 2009). …”

Section: Discussionmentioning

confidence: 60%

Growth condition optimization for docosahexaenoic acid (DHA) production by Moritella marina MP-1

Kautharapu

Rathmacher

Jarboe

2012

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…In future research, the FAS synthase inhibitors such as cerulenin and C75 a synthetic compound used as a fatty acid synthase inhibitor 21 , which have been reported to bind in equimolar ratios to β-keto-acyl-ACP synthase to block the interaction of malonyl-CoA, would be investigated to reduce UFA synthesis activity, thereby increasing PUFA synthesis. According to Ganuza 22 14,23,24 and Aurantiochytrium sp. SD116, prefer a salt concentration of 1.5 , while some Aurantiochytrium species produce high levels of DHA at low salinity.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp. SD116

Gao¹,

Song²,

Feng³

et al. 2013

J. Oleo Sci.

View full text Add to dashboard Cite

“…The DHA content in these species commonly reaches 50 of their total fatty acids 17,22 . In this study, similarly higher DHA contents were also observed in T. aureum and T. roseum, in which they were much higher than those in all of the other strains T. striatum and Schizochytrium spp.…”

Section: Discussionmentioning

confidence: 99%

Detection of Genes Involved in Fatty Acid Elongation and Desaturation in Thraustochytrid Marine Eukaryotes

et al. 2011

Self Cite

View full text Add to dashboard Cite

Optimization of Culture Conditions for Growth and Docosahexaenoic Acid Production by a Marine Thraustochytrid, Aurantiochytrium limacinum mh0186

Cited by 62 publications

References 25 publications

Growth condition optimization for docosahexaenoic acid (DHA) production by Moritella marina MP-1

Growth condition optimization for docosahexaenoic acid (DHA) production by Moritella marina MP-1

Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp. SD116

Detection of Genes Involved in Fatty Acid Elongation and Desaturation in Thraustochytrid Marine Eukaryotes

Contact Info

Product

Resources

About