Optimization of culture conditions and continuous production of chitosan by the fungi,Absidia coerulea

Kim, Woo Jun; Lee, Woo Gi; Théodore, K.; Chang, Ho Nam

doi:10.1007/bf02942243

Cited by 22 publications

(18 citation statements)

References 9 publications

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“…Chitin/chitosan creates side-stream product and additional value for the process. Although optimization of the biomass yield for chitin/chitosan and lipid co-production was not the primary goal of this study, there is a potential to enhance the yields by modification of C/N ratio, pH, aeration, cultivation temperature and time [22,98,99].…”

Section: Plos Onementioning

confidence: 99%

Microcultivation and FTIR spectroscopy-based screening revealed a nutrient-induced co-production of high-value metabolites in oleaginous Mucoromycota fungi

et al. 2020

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Mucoromycota fungi possess a versatile metabolism and can utilize various substrates for production of industrially important products, such as lipids, chitin/chitosan, polyphosphates, pigments, alcohols and organic acids. However, as far as commercialisation is concerned, establishing industrial biotechnological processes based on Mucoromycota fungi is still challenging due to the high production costs compared to the final product value. Therefore, the development of co-production concept is highly desired since more than one valuable product could be produced at the time and the process has a potentially higher viability. To develop such biotechnological strategy, we applied a high throughput approach consisting of micro-titre cultivation and FTIR spectroscopy. This approach allows single-step biochemical fingerprinting of either fungal biomass or growth media without tedious extraction of metabolites. The influence of two types of nitrogen sources and different levels of inorganic phosphorus on the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi was evaluated. FTIR analysis of biochemical composition of Mucoromycota fungi and biomass yield showed that variation in inorganic phosphorus had higher effect when inorganic nitrogen source-ammonium sulphate-was used. It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/ chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation. FTIR spectroscopy revealed the co-production potential of several

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Section: Plos Onementioning

confidence: 99%

Microcultivation and FTIR spectroscopy-based screening revealed a nutrient-induced co-production of high-value metabolites in oleaginous Mucoromycota fungi

et al. 2020

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“…and Zygorhynchus sp. [14] . The chitosan yield of Lentinula edodes was reported to be 20-50 mg/g dry weight [15] and the chitosan yield of Rhizopus oryzae was 270-700 mg/L [16] .…”

Section: Discussionmentioning

confidence: 99%

Production of chitosan from endolichenic fungi isolated from mangrove environment and its antagonistic activity

Logesh

Thillaimaharani

Sharmila

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

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“…(zygomycetes), A. niger (ascomycetes), Mucor rouxii (zygomycetes), Rhizophus oryzae (zygomycetes) and Lentinus edodes (basidiomycetes). [56][57][58][59] Nevertheless, the production of fungal chitin and chitosan has not yet been scaled up to the industrial level. 53 In addition to crustaceans and fungi, insects are another promising and sustainable source of chitin and chitosan, although they have not received much attention previously.…”

Section: Introductionmentioning

confidence: 99%

Current state of chitin purification and chitosan production from insects

Hahn

Tafi

Paul

et al. 2020

J of Chemical Tech & Biotech

203

113

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Chitin, and especially its deacetylated variant chitosan, has many applications, e.g. as carrier material for pharmaceutical drugs or as a flocculant in wastewater treatment. Despite its versatility and accessibility, chitin, the second most abundant polysaccharide on Earth, has so far been commercially extracted only from crustaceans and to a minor extent from fungi. Insects are a viable alternative source of chitin, but they have not been exploited in the past due to limited availability. Today however, for the sustainable production of animal feed, insect farming is being developed substantially. The availability of large quantities of insect biomass and chitin-rich side products such as exuviae and exoskeletons has been increasing. This review provides an overview of recently published studies of chitin extraction from insects, its subsequent conversion into chitosan and the primary analytical methods used to characterize insect-based chitin and chitosan. We have discovered a large number of research articles published over the past 20 years, confirming the increased attention being received by chitin and chitosan production from insects. Despite numerous publications, we identified several knowledge gaps, such as a lack of data concerning chitin purification degree and chitosan yield. Furthermore, analytical methods used to obtain physicochemical characteristics, structural information and chemical composition meet basic qualitative requirements but do not satisfy the need for a more quantitative evaluation. Despite the current shortcomings that need to be overcome, this review presents encouraging data on the use of insects as an alternative source of chitin and chitosan in the future.

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Optimization of culture conditions and continuous production of chitosan by the fungi,Absidia coerulea

Cited by 22 publications

References 9 publications

Microcultivation and FTIR spectroscopy-based screening revealed a nutrient-induced co-production of high-value metabolites in oleaginous Mucoromycota fungi

Microcultivation and FTIR spectroscopy-based screening revealed a nutrient-induced co-production of high-value metabolites in oleaginous Mucoromycota fungi

Production of chitosan from endolichenic fungi isolated from mangrove environment and its antagonistic activity

Current state of chitin purification and chitosan production from insects

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