Optimization of Cultural and Nutritional Parameters for the Production of Laccase by Pleurotus ostreatus ARC280

Elsayed, Maysa A.; Hassan, Mohamed M.; Elshafei, Ali M.; Haroun, Bakry M.; Othman, Abdelmageed M.

doi:10.9734/bbj/2012/1305

Cited by 33 publications

(30 citation statements)

References 63 publications

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“…Different studies have shown that laccase activity has regulated by copper ions through gene expression induction or translational or posttranslational regulation (Fonseca et al 2010). On the other hand, Elsayed et al (2012) supported our results. They reported that among the tested copper concentrations (50,100,250,500,750,1000,1250, and 1500 lM), 100 lM supported the maximum laccase production by P. ostreatus ARC280 compared to the original medium containing 50 lM Cu 2?…”

Section: Resultssupporting

confidence: 90%

“…The values are the mean ± SD for experiments of three separate experiments promoting laccase secretion by many fungal strains (Dekker et al 2007). Elsayed et al (2012) studied the effect of different concentration of Tween 80 (0.1-0.75%; v/v) on laccase activity, and they found that the enzyme activity reached to its maximum value at a concentration of 0.1% and increased by about 44% than control (without Tween-80). The increase in laccase activity in the presence of Tween 80 might be due to increased permeability of the cell membrane's lipid bilayer, facilitating more rapid secretion of enzymes out of the cell.…”

Section: Effect Of Tween 80 Concentrations On Laccase Production By Pmentioning

confidence: 99%

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Induction of fungal laccase production under solid state bioprocessing of new agroindustrial waste and its application on dye decolorization

Akpınar

Ürek

2017

3 Biotech

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Lignocellulosic wastes are generally produced in huge amounts worldwide. Peach waste of these obtained from fruit juice industry was utilized as the substrate for laccase production by Pleurotus eryngii under solid state bioprocessing (SSB). Its chemical composition was determined and this bioprocess was carried out under stationary conditions at 28°C. The effects of different compounds; copper, iron, Tween 80, ammonium nitrate and manganese, and their variable concentrations on laccase production were investigated in detail. The optimum production of laccase (43,761.33 ± 3845 U L -1 ) was achieved on the day of 20 by employing peach waste of 5.0 g and 70 lM ammonium nitrate, 750 lM Mn 2? as the inducers. The dye decolorization also researched to determine the degrading capability of laccase produced from peach culture under the above-mentioned conditions. Within this scope of the study, methyl orange, tartrazine, reactive red 2 and reactive black dyes were treated with this enzyme. The highest decolorization was performed with methyl orange as 43 ± 2.8% after 5 min of treatment when compared to other dyes. Up to now, this is the first report on the induction of laccase production by P. eryngii under SSB using peach waste as the substrate.

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Section: Resultssupporting

confidence: 90%

Section: Effect Of Tween 80 Concentrations On Laccase Production By Pmentioning

confidence: 99%

Induction of fungal laccase production under solid state bioprocessing of new agroindustrial waste and its application on dye decolorization

Akpınar

Ürek

2017

3 Biotech

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“…In agreement with the current study Elsayed et al (2012) reported that soluble starch was found to be the best carbon source for laccase production. However, galactose, fructose and maltose significantly repressed laccase formation by P. ostreatus which was in line with the current finding.…”

Section: The Most Preferred Carbon Sourcesupporting

confidence: 93%

“…Later, the contents were filtered with Whatman No.1 filter paper and the culture filtrate was centrifuged at 10,000 rpm for 15 min at 4°C to remove spores and insoluble. The clear supernatants (crude enzymes) were then collected in separate containers by decantation (Elsayed et al, 2012). This was used directly for the determination of laccase activity.…”

Section: Laccase Extractionmentioning

confidence: 99%

Selection and optimization of lignocellulosic substrate for laccase production from Pleurotus species

Edae¹,

Alemu²

2017

Int. J. Biotechnol. Mol. Biol. Res.

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The fruiting bodies of fungi were collected based on their morphology and inoculated on potato dextrose agar plate. Six different lignocellulosic substrates were collected and prepared for cultivation of Pleurotus species for laccase production. The highest enzyme production was obtained from bean straw compared to other substrates with an activity of 0.112 U/ml. The best three substrates; bean straw, Eucalyptus sawdust and wheat straw were selected, and a mixture of each of them on equal proportions was tested for laccase production potential. A mixture of Eucalyptus sawdust and bean straw on equal proportion was found to be the best, showing an activity of 0.137 U/ml and hence selected for different parameter optimization. Optimal laccase production (0.292 U/ml) was obtained on the 10th day of incubation period and the optimum temperature and pH were 28°C and 5.5, respectively. Soluble starch and peptone were found to be the most preferred carbon and nitrogen sources for laccase production, respectively. Asparagine and alanine induced more laccase production, with asparagine being the most potent inducer.

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“…Pseudomonas aeruginosa (MTCC no 2453) was cultivated LuriaBertani (LB) broth, minimal medium9 (M9), and asparagine-proline (AP) [17,18]. Asparagine proline broth allows high growth rate as it contains a strictly mineral base with asparagine as a sole source of nitrogen and glycerol as the carbon source.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Different Culture Media for Enhanced Production of Pseudomonas Aeruginosa (Mtcc No 2453) Biomass and Its Proteins

Neeraja

Tigulla

Sudhakar

2016

Int J Pharm Pharm Sci

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Objective: Microorganisms, especially bacteria and its proteins have proven to be potential anti-cancer agents as they selectively attack the tumor cells or tumor micro-environments. The extract of Pseudomonas aeruginosa found to contain proteins that have shown promising anticancer activity. In this work, it was attempted to increase the biomass and trigger the total protein fraction of Pseudomonas aeruginosa (MTCC 2453). Methods:The organism was cultivated in three different such as Luria-Bertani (LB) broth, minimal medium9 (M9), super broth medium (SB) and asparagine-proline (AP) broth. Asparagine proline broth was selected as it has shown high cell growth rate. The media was further optimized by the addition of NaHCO3 and copper sulphate to trigger the protein production. Optimized Aspergine proline broth has achieved highest cell biomass. After the shake flask culture, the overnight grown culture in optimized AP medium was further grown in a 5 L bioreactor by fed-batch cultivation to achieve higher cell densities. Results:The highest protein production was achieved at 40 ° C. Highest biomass and protein content was observed at pH 8 while lowest biomass was produced at pH 2. A gradual increase in biomass content observed from 12 h towards to 48 h. Conclusion:High biomass and proteins content and of Pseudomonas aeruginosa (MTCC 2453) can be produced in optimized asparagine-proline broth. Further the extract is purified to produce novel anti-cancer proteins.

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Optimization of Cultural and Nutritional Parameters for the Production of Laccase by Pleurotus ostreatus ARC280

Cited by 33 publications

References 63 publications

Induction of fungal laccase production under solid state bioprocessing of new agroindustrial waste and its application on dye decolorization

Induction of fungal laccase production under solid state bioprocessing of new agroindustrial waste and its application on dye decolorization

Selection and optimization of lignocellulosic substrate for laccase production from Pleurotus species

Evaluation of Different Culture Media for Enhanced Production of Pseudomonas Aeruginosa (Mtcc No 2453) Biomass and Its Proteins

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