Optimization of cultivation medium for enhanced production of antifungal metabolites by Streptomyces hygroscopicus

Grahovac, Jovana; Grahovac, Mila; Dodić, Jelena; Bajić, Bojana; Balaž, Jelica

doi:10.1016/j.cropro.2014.07.020

Cited by 38 publications

(25 citation statements)

References 23 publications

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“…The source and concentration of nutrients along with the cultural conditions are known to have mysterious effects on bioactive metabolite production in Streptomyces too, since the antibiotic production is not a static property [7]. Therefore, designing an appropriate medium and conditions for cultivation has prime importance in improving the antibiotic yield [8].…”

Section: Introductionmentioning

confidence: 99%

Enhanced antibacterial metabolite production through the application of statistical methodologies by a Streptomyces nogalater NIIST A30 isolated from Western Ghats forest soil

et al. 2017

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Streptomyces strains isolated from Nelliyampathy forest soil of Western Ghats, Kerala, India were evaluated for their antibacterial efficacy against two indicator pathogenic bacteria (Escherichia coli and Staphylococcus aureus). Among 140 strains tested, sixteen recorded potent antibacterial properties and were further screened against eleven bacterial pathogens. A strain identified as Streptomyces nogalater and designated as NIIST A30 exhibited maximum inhibition against all the test pathogens. Among the eight fermentation media tested, inorganic salts starch broth recorded the best for antibacterial production. The ethyl acetate crude extract exhibited antioxidant properties with IC50 value of 30 μg/mL and had no cytotoxicity towards L6, H9c2 and RAW 264.7 cell lines up to a concentration of 50 μg/mL. Maximum metabolite production was achieved in pH 7.0 at 35°C after 7 days incubation. The significant media components for maximum metabolite production were optimized through response surface methodology employing Plackett-Burman and Box-Behnken designs. The composition of the final optimized medium was soluble starch, 14.97g; (NH4)2SO4, 2.89g; K2HPO4, 2.07g; MgSO4.7H2O, 1g; NaCl, 1g, CaCO3, 2g; FeSO4.7H2O, 1mg; MnCl2.7H2O, 1mg; and ZnSO4.7H2O, 1mg per litre of distilled water. The optimization resulted an antibacterial activity of 28±1.5mm against S. epidermidis which was in close accordance with the predicted value of 30 mm. It is also evident from the result that an increase of 86.66% antibacterial production was recorded in optimized media. The chosen method was economical, efficient and useful for future antibacterial drug discovery from a broad spectrum metabolite producer like Streptomyces nogalater NIIST A30.

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Section: Introductionmentioning

confidence: 99%

Enhanced antibacterial metabolite production through the application of statistical methodologies by a Streptomyces nogalater NIIST A30 isolated from Western Ghats forest soil

et al. 2017

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“…Response surface methodology has several advantages that include less trials, suitability for multiple factor experiments, determination of relativity between factors, defining the most suitable conditions, and forecast responses. The method is widely used in optimization of microbial fermentation processes (Chang et al 2006;Wang et al 2008Wang et al , 2010aWang et al 2011;Grahovac et al 2014;Kong et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Optimization of Nutrient and Fermentation Parameters for Antifungal Activity by Streptomyces lavendulae Xjy and Its Biocontrol Efficacies against Fulvia fulva and Botryosphaeria dothidea

Gao

Jiang

et al. 2015

Journal of Phytopathology

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This study has been proved that statistical experimental designs offer efficient and feasible approaches for determination of culture medium and fermentation conditions of Streptomyces lavendulae Xjy. Nutrition and cultural conditions have high influence on the antibiotic production of S. lavendulae Xjy. Soya bean meal, NaCl and (NH4)2SO4 were identified as the best ingredients for high antifungal activity of Xjy based on single variable experiments. Using response surface methodology, the best concentrations of medium ingredients were 1.46% soya bean meal, 2.25% NaCl and 1.26% (NH4)2SO4. The potential components were reduced from seven to three, and the antibiotic activity was increased by 10%. The optimized conditions for Xjy in a 250‐ml Erlenmeyer flask were initial pH 5, medium volume 25 ml/250 ml and inoculation volume 10% at 30°C with 200 rpm by response surface methodology. Validation experiments carried out to check the accuracy of the models indicated that the predicted values agreed with the experimental values. The extension of necrosis lesion of Botryosphaeria dothidea was impeded by the application of Xjy in apple fruit. In tomato plants, Xjy showed the 43.7–51.7% curative and protective effects against Fulvia fulva. The optimal medium not only gave a 20% increase in antifungal activity, but it provided a simpler formulation. The results will set a basis for further study on large‐scale fermentation volumes using S. lavendulae Xjy and be useful for the development of more advanced control strategies on plant diseases.

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“…The supernatants of the five media used to cultivate the FA13 strain could all efficiently suppress the production of norsolorinic acid, indicating that the FA13 strain could grow on a broad range of nutrient substances and produce active metabolites that inhibit the mycelial growth of A. parasiticus and production of aflatoxin. In order to enhance the yield of anti-fungal substances, many researchers used response surface methodology to optimize cultivation media and cultivation conditions [20][21][22]. Uniform design was first proposed by Wang yuan and Fang kaitai in 1978 [23].…”

Section: Discussionmentioning

confidence: 99%

Isolation of a novel deep-sea Bacillus circulus strain and uniform design for optimization of its anti-aflatoxigenic bioactive metabolites production

et al. 2019

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2019) Isolation of a novel deep-sea Bacilluscirculus strain and uniform design for optimization of its anti-aflatoxigenic bioactive metabolites production, Bioengineered, 10:1, 13-22, ABSTRACTThe deep-sea bacterium strain FA13 was isolated from the sediment of the South Atlantic Ocean and identified as Bacillus circulans based on 16S ribosomal DNA sequence. Through liquid fermentation with five media, the cell-free supernatant fermented with ISP2 showed the highest inhibition activities against mycelial growth of Aspergillus parasiticus mutant strain NFRI-95 and accumulation of norsolorinic acid, a precursor for aflatoxin production. Based on ISP2, uniform design was used to optimize medium formula and fermentation conditions. After optimization, the inhibition efficacy of the 20-time diluted supernatant against A. parasiticus NFRI-95 mycelial growth and aflatoxin production was increased from 0-23.1% to 100%. Moreover, compared to the original protocol, medium cost and fermentation temperature were significantly reduced, and dependence on seawater was completely relieved, thus preventing the fermentor from corrosion. This is the first report of a deep-sea microorganism which can inhibit A. parasiticus NFRI-95 mycelial growth and aflatoxin production. ARTICLE HISTORY

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Optimization of cultivation medium for enhanced production of antifungal metabolites by Streptomyces hygroscopicus

Cited by 38 publications

References 23 publications

Enhanced antibacterial metabolite production through the application of statistical methodologies by a Streptomyces nogalater NIIST A30 isolated from Western Ghats forest soil

Enhanced antibacterial metabolite production through the application of statistical methodologies by a Streptomyces nogalater NIIST A30 isolated from Western Ghats forest soil

Optimization of Nutrient and Fermentation Parameters for Antifungal Activity by Streptomyces lavendulae Xjy and Its Biocontrol Efficacies against Fulvia fulva and Botryosphaeria dothidea

Isolation of a novel deep-sea Bacillus circulus strain and uniform design for optimization of its anti-aflatoxigenic bioactive metabolites production

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