Optimization of cultivation conditions for fungichromin production from Streptomyces padanus PMS-702

Wu, Jonny; Huang, Jenn-Wen; Shih, Hsin-Der; Lin, Wei; Liu, Yung-Chuan

doi:10.1016/j.jcice.2007.11.006

Cited by 25 publications

(16 citation statements)

References 11 publications

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“…In fact, soybean powder has a high level of proteins ranging from 36 % to 40 % which contains necessary amino acids for the cell growth such as glutamic acid, aspartic acid, cysteine, vitamins and mineral salts. Therefore, soybean powder has been favored as one of the main ingredients in antibiotic-producing media for many Streptomyces species in many studies before [11]. Nevertheless, in agreement with previous studies, the present study showed that other nitrogen sources like yeast extract, tryptone, malt extract, peptone and casein were also appropriate for the antibiotic biosynthesis by Streptomyces [12].…”

Section: Effect Of Carbon and Nitrogen Sourcessupporting

confidence: 89%

OPTIMAL FERMENTATION CONDITIONS FOR ANTIBIOTIC PRODUCTION BY ENDOPHYTIC Streptomyces cavourensis YBQ59 ISOLATED FROM Cinnamomum cassia Presl

Vu¹,

Nguyen²,

Le³

et al. 2019

JST

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This study determined the optimal nutrient and environmental conditions for the production of antimicrobial and cytotoxic agents by Streptomyces cavourensis YBQ59 during the fermentation process. The antimicrobial and cytotoxic activities of eluted fractions based gradient solvents via silica gel column of chromatograph were also evaluated. S. cavourensis YBQ59 exhibited strong, board-spectrum antimicrobial activities under fermentation conditions as follows: MT6 medium with soluble starch and soybean powder as carbon and nitrogen sources, respectively, temperature 30oC, initial pH 7.0, 20% DO concentration and with 5% initial seed culture. The kinetic of fermentation showed that the antimicrobial and cytotoxic activities were highest at between 72h and 78h. The fraction 25/1 D-M (dichloromethane –methanol) exhibited the highest antimicrobial effect against methicillin-resistant Staphylococcus aureus ATCC 33591 and methicillin-resistant Staphylococcus epidermidis ATCC 35984 with minimum inhibitory concentrations of 9.4 µg/ml and 6.4 µg/ml, respectively. The fraction 10/1 D-M had strong cytotoxic effects towards multidrug-resistant A459 and H1299 lung carcinoma cell lines with the cell viability of 11.3% and 12.4%, respectively. In conclusion, S. cavourensis YBQ59 would be a potential producer of valuable bioactive compounds that may have a board application in pharmaceutical industry and agriculture (biocontrol, livestock, food safety and quality management)

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Section: Effect Of Carbon and Nitrogen Sourcessupporting

confidence: 89%

OPTIMAL FERMENTATION CONDITIONS FOR ANTIBIOTIC PRODUCTION BY ENDOPHYTIC Streptomyces cavourensis YBQ59 ISOLATED FROM Cinnamomum cassia Presl

Vu¹,

Nguyen²,

Le³

et al. 2019

JST

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“…In order to examine the growth of S. hygroscopicus and production of antifungal metabolites against A. alternata and F. avenaceum on different nitrogen sources, shake flask cultures were carried out. The selection of mostly used nitrogen sources in biotechnological production was tested (Wu et al 2008;Ripa et al 2009). As expected, the evaluated treatments had significant (p<0.001) influence on the mycelial growth inhibition zone diameter (mm) for both tested fungi.…”

Section: Results and Discusionsmentioning

confidence: 62%

“…A2D (Singh et al 2009). Also, Wu et al reported that soybean meal was an excellent nitrogen source for production of fungichromin by S. padanus PMS-702 (Wu et al 2008). In general, nitrogen from soybean meal derivatives are extensively utilized in the fermentation processes (Portera and Jones, 2003;Ortiz et al 2007).…”

Section: Results and Discusionsmentioning

confidence: 99%

Effect of nitrogen sources on the production of antifungal metabolites by Streptomyces hygroscopicus

Mitrović

Grahovac

Dodić

et al. 2017

Zb Mat srp prir nauk

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Biotechnologically produced antifungal compounds for control of plant diseases caused by phytopathogenic fungi represent a promising alternative to synthetic pesticides. Fungi from the genera Alternaria and Fusarium are listed among important storage pathogens of apple fruits. A. alternata causes significant annual losses of apple fruit. Also, F. avenaceum is one of the most commonly encountered Fusarium species identified as the causal agent of a wet apple core rot. Species of the Streptomyces genera are soil bacteria that produce significant quantities of bioactive compounds in appropriate media. Defining the culture medium composition is the basis of bioprocesses development. Nitrogen source is critical component of cultivation medium and also the most useful tool for stimulation of the antifungal metabolites production. The aim of this study was to select the best nitrogen sources in medium for the production of antifungal compounds effective against A. alternata and F. avenaceum by S. hygroscopicus. Activity of the cultivation liquids on A. alternata and F. avenaceum isolates was tested in vitro using well diffusion method. The results indicate that maximum inhibition zone was reached in medium with soybean meal as nitrogen source with inhibition diameter more than 35 mm for both tested fungi.

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“…was good with glucose 27,28 while Streptomyces hygroscopicus CH-7 utilized lactose as carbon source for antibiotic production 29 . In order to make effective composition of growth medium, the different nitrogen sources were evaluated for their influence on biomass and antimicrobial metabolite production by the strain VLK-4.…”

Section: Effect Of Carbon and Nitrogen Sources On Biomass And Bioactimentioning

confidence: 99%

Untitled

2014

IJPSR

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ABSTRACT:The aim of the present study was to design the suitable culture medium and also optimize the culture conditions for enhanced production of antimicrobial metabolites by Streptomyces violaceoruber VLK-4 which exhibited a broad spectrum of activity against bacteria and fungi. Production of bioactive metabolites by the strain was high in modified yeast extract-malt extract dextrose (ISP-2) broth as compared to other media tested. Mannitol (0.4%) and asparagine (1%) were found to be the most suitable carbon and nitrogen sources for the optimum production of bioactive metabolites as well as growth. Maximum production of bioactive metabolites was found in the culture medium with initial pH 7.0 incubated for five days at 30 o C under shaking conditions. This is the first report on the optimization of bioactive metabolites by Streptomyces violaceoruber VLK-4

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Optimization of cultivation conditions for fungichromin production from Streptomyces padanus PMS-702

Cited by 25 publications

References 11 publications

OPTIMAL FERMENTATION CONDITIONS FOR ANTIBIOTIC PRODUCTION BY ENDOPHYTIC Streptomyces cavourensis YBQ59 ISOLATED FROM Cinnamomum cassia Presl

OPTIMAL FERMENTATION CONDITIONS FOR ANTIBIOTIC PRODUCTION BY ENDOPHYTIC Streptomyces cavourensis YBQ59 ISOLATED FROM Cinnamomum cassia Presl

Effect of nitrogen sources on the production of antifungal metabolites by Streptomyces hygroscopicus

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