Optimization of cryopreservation condition for hematopoietic stem cells from umbilical cord blood

Son, Jeong Hwa; Heo, Yoon Jeong; Park, Mi Young; Kim, Hyung Hoi; Lee, Kyu Sup

doi:10.1016/j.cryobiol.2010.01.007

Cited by 16 publications

(7 citation statements)

References 30 publications

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“…The reduction was observed for all colony types in both the microscope as well as the digital review (note: the CFU-GEMMs were not counted in the microscope review). This overall reduction in colony count is consistent with what other groups have observed in studies evaluating different cryopreservation methods (9,10). These data also demonstrate less variability between reviewers in cultures of the frozen samples from those of the fresh samples.…”

Section: Discussionsupporting

confidence: 90%

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Variability in subjective review of umbilical cord blood colony forming unit assay

Powell

Kwee

Nutter

et al. 2016

Cytometry Part B Clinical

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Section: Discussionsupporting

confidence: 90%

“…This overall reduction in colony count is consistent with what other groups have observed in studies evaluating different cryopreservation methods (9,10). The mean number of total colonies based on the reviewers' microscope counts for fresh and frozen samples was 61and 43, respectively.…”

Section: Discussionsupporting

confidence: 89%

Variability in subjective review of umbilical cord blood colony forming unit assay

Powell

Kwee

Nutter

et al. 2016

Cytometry Part B Clinical

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“…In this study, the three cryoprotectants were assessed. Most cord blood banks currently use 10% ethylene glycol (EG) and 2.0% DMSO (v/v) [ 20 ], 10% DMSO (v/v) and 2.0% dextran-40 [ 21 ], and 2.5% DMSO (v/v) + 30 mmol/L trehalose [ 21 ]. 115 UCB units were processed and cryopreserved.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord Blood

Chen

Yue

Ruan

et al. 2015

Stem Cells International

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Purpose. Cryoprotectants (CPA) for stem cells from umbilical cord blood (UCB) have been widely developed based on empirical evidence, but there is no consensus on a standard protocol of preservation of the UCB cells. Methods. In this study, UCB from 115 donors was collected. Each unit of UCB was divided into four equal parts and frozen in different kinds of cryoprotectant as follows: group A, 10% ethylene glycol and 2.0% dimethyl sulfoxide (DMSO) (v/v); group B, 10% DMSO and 2.0% dextran-40; group C, 2.5% DMSO (v/v) + 30 mmol/L trehalose; and group D, without CPA. Results. CD34+, cell viability, colony forming units (CFUs), and cell apoptosis of pre- and postcryopreservation using three cryoprotectants were analyzed. After thawing, significant differences in CD34+ count, CFUs, cell apoptosis, and cell viability were observed among the four groups (P < 0.05). Conclusion. The low concentration of DMSO with the addition of trehalose might improve the cryopreservation outcome.

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“…35 This has resulted in the need for development of an alternative cryopreservation medium or a cryopreservation medium consisting of low concentration of DMSO. 86 In general, FBS is routinely added to stabilize the cell membranes and adjust intra-and extracellular osmotic pressure, thus maintaining the survival rate of cells. However, the use of FBS in cryopreservation media should be minimized due to its potential to trigger a xenogeneic immune response or transmit pathogens to the recipient.…”

Section: Slow Freezingmentioning

confidence: 99%

Cryopreservation of Human Mesenchymal Stem Cells for Clinical Applications: Current Methods and Challenges

Yong

Zaman

Xufeng

et al. 2015

Biopreservation and Biobanking

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Mesenchymal stem cells (MSCs) hold many advantages over embryonic stem cells (ESCs) and other somatic cells in clinical applications. MSCs are multipotent cells with strong immunosuppressive properties. They can be harvested from various locations in the human body (e.g., bone marrow and adipose tissues). Cryopreservation represents an efficient method for the preservation and pooling of MSCs, to obtain the cell counts required for clinical applications, such as cell-based therapies and regenerative medicine. Upon cryopreservation, it is important to preserve MSCs functional properties including immunomodulatory properties and multilineage differentiation ability. Further, a biosafety evaluation of cryopreserved MSCs is essential prior to their clinical applications. However, the existing cryopreservation methods for MSCs are associated with notable limitations, leading to a need for new or improved methods to be established for a more efficient application of cryopreserved MSCs in stem cell-based therapies. We review the important parameters for cryopreservation of MSCs and the existing cryopreservation methods for MSCs. Further, we also discuss the challenges to be addressed in order to preserve MSCs effectively for clinical applications.

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Optimization of cryopreservation condition for hematopoietic stem cells from umbilical cord blood

Cited by 16 publications

References 30 publications

Variability in subjective review of umbilical cord blood colony forming unit assay

Variability in subjective review of umbilical cord blood colony forming unit assay

Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord Blood

Cryopreservation of Human Mesenchymal Stem Cells for Clinical Applications: Current Methods and Challenges

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