Optimization of citric acid production by Aspergillus niger using two downgraded Algerian date varieties

Abstract

In the present work, the GHARS and the MECH DEGLA downgraded date varieties were used in a fermentation medium in order to produce citric acid by the Aspergillus niger . The biochemical characteristics of the dates were investigated, along with the chemical and physical characteristics of the solutions of both samples. The analyzed parameters included the moisture and sugar content, the ash residual, the pH values, and the electrical conductivity. The effect of the following fermentation parameters was studied: initial pH, temperature, incubation period, and methanol. For the GHARS and MECH DEGLA date varieties respectively, the ash residual measured at 1.90% and 2.47%. For each date variety, the moisture and total sugars were measured at 11.59% and 85%, for the GHARS, and 12.82% and 80.47% for the MECH DEGLA. Citric acid production using either of the two varieties of dates showed a high yield in a short time. The obtained results showed that the highest production of citric acid by both medium of dates was achieved at the initial pH value of 3.0, temperature 30 °C, and an incubation period of 8 days. Also, the maximum amount of citric acid was produced when both mediums contained 4% of methanol. Both varieties of dates showed a good yield for the citric acid and can be used as a culture medium since they are economic and ensure good growth for the Aspergillus niger.

Optimization of Medium Constituents for the Production of Citric Acid from Waste Glycerol Using the Central Composite Rotatable Design of Experiments

“…On the other hand, Hu et al, in a study with the same strain, obtained a yield of 74.9% [ 53 ]. In submerged cultures of Aspergillus niger using two types of dates, GHARS and MECH DEGALA, as a carbon source, the concentration of citric acid was 42.25 g∙L −1 and 36.60 g∙L −1 , respectively [ 54 ]. In a study with beet molasses as a substrate, citric acid concentrations of 19.13 g∙L −1 and 34.62 g∙L −1 were obtained with a sugar concentration of 200 g∙L −1 and 150 g∙L −1 , respectively [ 55 ].…”

Citric Acid: Properties, Microbial Production, and Applications in Industries

“…The search for citric acid biosynthesis substrates involving Aspergillus niger has been the subject of extensive research [ 54 , 123 , 132 , 133 , 134 , 135 , 136 , 137 , 138 , 139 , 140 , 141 ]. High-purity substrates such as sucrose, glucose, fructose, and maltose can be utilized in citric acid production.…”

Peningkatan Nilai Tambah Onggok Singkong dan Dedak Padi Sebagai Substrat pada Produksi Asam Sitrat

“…Senyawa ini diproduksi melalui proses fermentasi dengan tingkat produksi berkisar antara 1,4-1,7 juta ton per tahun (Adeoye et al, 2015) dan tingkat permintaan berkisar 3,5-5,0% per tahun (Roukas & Kotzekidou, 2020). Pada umumnya, asam sitrat digunakan dalam industri makanan dan minuman, farmasi/kimia, tekstil, kosmetik, serta deterjen (Show et al, 2015;Chergui et al, 2021). Menurut Vandenberghe et al (2017), sebanyak 70% dari total produksi asam sitrat digunakan industri makanan, 12% digunakan industri farmasi, dan 18% diaplikasikan pada industri lainnya,.…”