Optimization of Callus and Cell Suspension Cultures of Lycium schweinfurthii for Improved Production of Phenolics, Flavonoids, and Antioxidant Activity

Mamdouh, Diaa; Smetanska, Iryna

doi:10.3390/horticulturae8050394

Cited by 10 publications

(9 citation statements)

References 80 publications

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“…The plant material of L. schweinfurthii callus cultures was obtained from leaf explants according to the method of Mamdouh and Smetanska (2022). The obtained callus was sub-cultured on MS (Murashige and Skoog) medium (Duchefa, Haarlem, The Netherlands) supplemented with 2 mg L − 1 NAA (Mallinckrodt, Hazelwood, MO), 30 g L − 1 sucrose, and 8 g L − 1 solidifying agar (Duchefa).…”

Section: Plant Materialsmentioning

confidence: 99%

Production of phenolic compounds, flavonoids, and lupeol by Lycium schweinfurthii suspension cultures in shake flasks and a rocking-motion bioreactor

Mamdouh,

Nebauer,

Junne

et al. 2024

Preprint

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Lycium schweinfurthii, a wild shrub of the Solanaceae family, has received increasing attention in the last decade for its therapeutic potential in traditional medicine due to its diverse array of secondary metabolites, including phenolic substances and terpenoids. The aim of this study was to investigate the accumulation of phenolics, flavonoids, and the terpenoid lupeol in L. schweinfurthii cell suspension cultures cultivated in flasks and in a single-use 2-dimensional rocking motion bioreactor. 3 different media formulations were compared for in vitro cell cultures. Various parameters, such as biomass accumulation, settled cell volume, cell viability (assessed via a 2,3,5-triphenyl tetrazolium chloride assay), and sucrose consumption were recorded as indicators of cell activity and growth. Total phenolic and flavonoid contents were estimated spectrophotometrically and lupeol was quantified via High-Performance Thin Layer Chromatography (HPTLC). Although a higher fresh biomass concentration of 464 g L− 1 was obtained in MS medium supplemented with a combination of each, 1 mg L− 1 of 2,4-Dichlorophenoxyacetic acid (2,4-D) and 1-Naphthaleneacetic acid (NAA), the rocking-motion bioreactor cultivation was performed with 2 mg L− 1 NAA due to its superior reproducibility in viability, productivity, and content of bioactive compounds. A final fresh biomass concentration of 185 g L− 1 was achieved in a 16 L cultivation scale with a notable increase in the concentration of phenolics (1.4-fold) and flavonoids (1.7-fold). Most importantly, the concentration of lupeol, a pentacyclic triterpenoid known for its anti-inflammatory, antibacterial, and anti-atherogenic properties, exhibited a remarkable 5.5-fold increase in the bioreactor cultivation (585 µg g− 1) compared to shake flask cultivations (106 µg g− 1). The current study demonstrated the profound impact of media composition and especially of controlled cultivation conditions in a rocking-motion bioreactor on the accumulation of bioactive compounds. The findings are also relevant for other plant cell cultures.

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Section: Plant Materialsmentioning

confidence: 99%

Production of phenolic compounds, flavonoids, and lupeol by Lycium schweinfurthii suspension cultures in shake flasks and a rocking-motion bioreactor

Mamdouh,

Nebauer,

Junne

et al. 2024

Preprint

Self Cite

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“…L. schweinfurthii is a member of the Solanaceae family that is naturally distributed along the African coastal area, Sicily, Cyprus, and Crete (Boulos, 2002). Its leaves and fruits are used in traditional medicine due to its contents of alkaloids, glycosides, sterols, saponins, resins, phenolics, and flavonoids (Mamdouh and Smetanska, 2022). Although the limited distribution areas, Shaltout et al (2018) reported that this species is distributed in many habitats (coastal sand dunes, flat sand sheets, roadsides, island sand dunes, and canal banks) along the Mediterranean coast in Egypt.…”

Section: Study Speciesmentioning

confidence: 99%

Wood anatomy and dendrochronological potentiality of some woody shrubs from the southern Mediterranean coast in Egypt

Farahat

Gärtner

2023

Front. Plant Sci.

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In tropical and subtropical regions, much research is still required to explore the dendrochronological potential of their trees. This study aims to evaluate the anatomical structure and dendrochronological potential of three Mediterranean desert shrubs in Egypt (Lycium schweinfurthii var. schweinfurthii, L. europaeum, and Calligonum polygonoides subsp. comosum) supported by X-ray density. The results showed that the target species had distinct growth rings at macroscopic and microscopic levels. The vessel traits reflected the adaptability of each species with the prevailing arid climate conditions. After the exclusion of the non-correlated series, we obtained three site chronologies that cover the years 2013-2022 for L. schweinfurthii, 2012-2022 for L. europaeum, and 2011-2022 for C. comosum. The mean series intercorrelation was 0.746, 0.564, and 0.683 for L. schweinfurthii, L. europaeum, and C. comosum, respectively. The EPS (expressed population signal) values ranged from 0.72 to 0.80, while the SNR (species-to-noise ratio) ranged from 9.1 to 21.5. Compiling all series of L. schweinfurthii raised the EPS value to 0.86. The chronologies developed for the studied species were relatively short since we dealt with multi-stemmed shrubs. The average percentage difference between latewood density (LWD) and earlywood density (EWD) in C. comosum, L. europaeum, and L. schweinfurthii were 11.8% ± 5.5, 5.2%± 1.87, and 3.6% ± 1.86, respectively. X-ray densitometry helped in the precise determination of the ring borders of the studied species. The relationships between the radial growth of the studied species and the climate variables were weak to moderate but mostly not significant (i.e., r < 0.7). Generally, the radial growth of the target species had a weak to moderate positive correlation with temperature and precipitation during the wet season (winter), while negatively correlated with temperature for the rest of the year, particularly in summer. Our data agrees with earlier findings that ring formation starts at the beginning of the long vegetative stage, then the rest of the assimilated carbohydrates are directed to the flowering and fruiting at the end of the vegetative stages. For more efficient dendrochronological studies on subtropical and Mediterranean trees, we recommend carrying out xylogenesis studies, collection of phenological data, sampling 45-80 trees per species, using new techniques, and choosing homogeneous and close sites for wood sampling.

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“…According to the authors [86], such results can be useful in the large-scale production of phenols with antioxidant activity. Another biotechnological way to produce secondary metabolites is through in vitro micropropagated plants, and this approach was also made by the same group [88], who developed a protocol for in vitro micropropagation of L. schweinfurthii with genetic stability, assessed through a random amplified polymorphic DNA (RAPD), inter-simple sequence repeats (ISSR), and one biochemical technique, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), able to scavenge free radicals and produce phenols. The leaves of the micropropagated plants produced phenols, measured as total phenols and total flavonoids, and one phenolic acid (ferulic acid) was identified by high performance thin-layer chromatography (HPTLC).…”

Section: Biotechnological Production Of Secondary Metabolitesmentioning

confidence: 99%

“…The capacity for scavenging the DPPH and ABTS free radicals were IC 50 = 0.43 and 1.99 mg/mL, respectively. As aforementioned, the authors [88] considered that micropropagated plants of L. schweinfurthii can be another way to obtain plant material for in vitro secondary metabolite production. Nevertheless, nothing is reported about the production of clones with the best capacity for accumulating the secondary metabolites with antioxidant activity.…”

Section: Biotechnological Production Of Secondary Metabolitesmentioning

confidence: 99%

Chemical and Biological Properties of Three Poorly Studied Species of Lycium Genus—Short Review

Miguel

2022

Metabolites

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The genus Lycium belongs to the Solanaceae family and comprises more than 90 species distributed by diverse continents. Lycium barbarum is by far the most studied and has been advertised as a “superfood” with healthy properties. In contrast, there are some Lycium species which have been poorly studied, although used by native populations. L. europaeum, L. intricatum and L. schweinfurthii, found particularly in the Mediterranean region, are examples of scarcely investigated species. The chemical composition and the biological properties of these species were reviewed. The biological properties of L. barbarum fruits are mainly attributed to polysaccharides, particularly complex glycoproteins with different compositions. Studies regarding these metabolites are practically absent in L. europaeum, L. intricatum and L. schweinfurthii. The metabolites isolated and identified belong mainly to polyphenols, fatty acids, polysaccharides, carotenoids, sterols, terpenoids, tocopherols, and alkaloids (L. europaeum); phenolic acids, lignans, flavonoids, polyketides, glycosides, terpenoids, tyramine derivatives among other few compounds (L. schweinfurthii), and esters of phenolic acids, glycosides, fatty acids, terpenoids/phytosterols, among other few compounds (L. intricatum). The biological properties (antioxidant, anti-inflammatory and cytotoxic against some cancer cell lines) found for these species were attributed to some metabolites belonging to those compound groups. Results of the study concluded that investigations concerning L. europaeum, L. intricatum and L. schweinfurthii are scarce, in contrast to L. barbarum.

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Optimization of Callus and Cell Suspension Cultures of Lycium schweinfurthii for Improved Production of Phenolics, Flavonoids, and Antioxidant Activity

Cited by 10 publications

References 80 publications

Production of phenolic compounds, flavonoids, and lupeol by Lycium schweinfurthii suspension cultures in shake flasks and a rocking-motion bioreactor

Production of phenolic compounds, flavonoids, and lupeol by Lycium schweinfurthii suspension cultures in shake flasks and a rocking-motion bioreactor

Wood anatomy and dendrochronological potentiality of some woody shrubs from the southern Mediterranean coast in Egypt

Chemical and Biological Properties of Three Poorly Studied Species of Lycium Genus—Short Review

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