Optimization of bud induction in cotyledonary explants of Pinus canariensis

Pulido, Carolina Martínez; Harry, Indra S.; Thorpe, Trevor A.

doi:10.1007/bf00034360

Cited by 27 publications

(7 citation statements)

References 14 publications

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“…Increasing concentrations of growth regulators caused the production of hard and compact callus from which only small numbers of weak, malformed, and hyperhydrated microshoots could be regenerated. Therefore, in line with previous reports in coniferous species (Pulido et al 1992;Ravindra et al 2005;Tang and Newton 2005), optimum combinations of PGRs were essential for callus induction in C. lanceolata. The 8-wk-old compact callus induced on DCR medium supplemented with 2.0 mg l −1 BA, 0.008 mg l −1 TDZ, and 0.2 mg l −1 NAA were transferred to DCR medium supplemented with BA or KT at different concentrations to induce organogenesis (Table 5).…”

Section: Resultssupporting

confidence: 91%

Efficient organogenesis and plantlet regeneration in the timber species Cunninghamia lanceolata (Lamb.) Hook

Zhu

Wang

et al. 2007

In Vitro Cell.Dev.Biol.-Plant

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Two efficient regeneration systems were developed in Cunninghamia lanceolata, the most important conifer for industrial wood production in China. Cotyledons and hypocotyls derived from greenhouse-grown seedlings were used as initial explants in our research. A high frequency (95.1±1.84%) of adventitious buds were initiated directly from cotyledons cultured on Douglas-fir cotyledon revised (DCR) medium supplemented with 1 mg l −1 benzyladenine (BA), 0.1 mg l −1 α-naphthaleneacetic acid (NAA), and 0.004 mg l −1 thidiazuron (TDZ) with a maximum mean number of adventitious buds per cotyledon explant of 3.76±0.08. In contrast, a high percentage (93.73± 0.55%) of adventitious buds regenerated via callus produced from hypocotyls cultured on DCR medium supplemented with plant growth regulators with a maximum number of adventitious buds per explant (16.71±0.34). Adventitious buds elongated on DCR medium supplemented with 0.2 mg l −1 BA and 0.02 mg l −1 NAA. After liquid pretreatment with 50 mg l −1 indole-3-butyric acid (IBA), over 95% of the shoots successfully rooted on ½ DCR medium supplemented with 0.3 mg l −1 IBA. The innovated systems reported in this study will be useful tools for future genetic manipulation of C. lanceolata and may be adapted for large-scale propagation in other conifers.

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Section: Resultssupporting

confidence: 91%

Efficient organogenesis and plantlet regeneration in the timber species Cunninghamia lanceolata (Lamb.) Hook

Zhu

Wang

et al. 2007

In Vitro Cell.Dev.Biol.-Plant

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“…For instance, for explants of P.canariensis the best exposure time was 4-8 h in 22.5 mg/l BA solution [3]. High concentrations or prolonged exposure can completely suppress shoot formation.…”

Section: Results and Conclusionmentioning

confidence: 99%

Effect of PGR pulse treatments and ethylene precursor and inhibitors on Scots pine micropropagation

Lebedev

Shestibratov

2011

BMC Proc

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“…The combination of these factors offers a realistic assessment of the efficacy of the culture conditions (Martínez Pulido et al 1992). The lowest SEC values were found in NG somatic embryos treated with 1 lM BA.…”

Section: Discussionmentioning

confidence: 99%

A combined pathway of somatic embryogenesis and organogenesis to regenerate radiata pine plants

Montalbán

Diego

Igartua

et al. 2011

Plant Biotechnol Rep

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This study describes for the first time in Pinus genus a plant regeneration system via a combined pathway of somatic embryogenesis and organogenesis from immature seeds of radiata pine. Somatic embryos were obtained from embryogenic line 2162 of Pinus radiata D. Don on EDM basal medium containing 60 lM ABA and 6% sucrose. The explants used for organogenesis experiments were either freshly collected somatic embryos or somatic embryos germinated for 1 week. Germination medium was half-strength LP medium, supplemented with 0.2% activated charcoal. Different induction periods and BA concentrations were assayed for shoot induction. After induction treatments, explants were elongated on the same medium used for germination stage. Rooting medium was quarter-strength LP medium supplemented with three different auxin treatments: 1.5 mg L -1 1-naphthalene acetic acid (NAA), 1.5 mg L -1 indole-3-butyric acid (IBA) and 1 mg L -1 IBA with 0.5 mg L -1 NAA (MIX). The effect of the photon flux (120 mmol m -2 s -1 and darkness) in the first week of the explants in the rooting media was also tested. This methodology could offer an alternative to overcome some problems associated with somatic embryogenesis such as the seasonality of embryogenic tissue (ET) initiation or a low embryo production from the ET, a particularly important issue in the case of genetically transformed ETs.

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Optimization of bud induction in cotyledonary explants of Pinus canariensis

Cited by 27 publications

References 14 publications

Efficient organogenesis and plantlet regeneration in the timber species Cunninghamia lanceolata (Lamb.) Hook

Efficient organogenesis and plantlet regeneration in the timber species Cunninghamia lanceolata (Lamb.) Hook

Effect of PGR pulse treatments and ethylene precursor and inhibitors on Scots pine micropropagation

A combined pathway of somatic embryogenesis and organogenesis to regenerate radiata pine plants

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