Optimization of Bioethanol Production from Enzymatic Treatment of Argan Pulp Feedstock

Abstract

Argan pulp is an abundant byproduct from the argan oil process. It was investigated to study the feasibility of second-generation bioethanol production using, for the first time, enzymatic hydrolysis pretreatment. Argan pulp was subjected to an industrial grinding process before enzymatic hydrolysis using Viscozyme L and Celluclast 1.5 L, followed by fermentation of the resulting sugar solution by Saccharomyces cerevisiae. The argan pulp, as a biomass rich on carbohydrates, presented high saccharification yields (up to 91% and 88%) and an optimal ethanol bioconversion of 44.82% and 47.16% using 30 FBGU/g and 30 U/g of Viscozyme L and Celluclast 1.5 L, respectively, at 10%w/v of argan biomass.

The Study of the Heterogeneity of the Qualities of Argan Oils and Pomaces from Different Cooperatives in the Essaouira Region (Morocco)

“…To summarize, as clearly indicated in the two graphs from Fig. 2, the contents of total soluble sugars and total reducing sugars of the two categories of Argan pomace (P-UA-ME and P-RA-AE) are lower than those of Argan pulp as reported by Zeghlouli et al 39) . Generally, the obtained results of total sugars and total reducing sugars contents make Argan pomace very useful in several fields.…”

Innovation of argan (Argania spinosa (L.) Skeels) products and byproducts for sustainable development of rural communities in Morocco. A systematic literature review

“…Some studies published in the last five years (Rahib et al 2019(Rahib et al , 2021 highlighted their potential use as an innovative source of bioenergy; ANS can represent a low-cost biomass that should be a promising biofuel in combustion systems or a source of antioxidant additives for biofuels (Afailal et al 2021). Argan pulp too can be used as an innovative and low-cost biomass for the bioethanol production (Zouhair et al 2019); According to Zeghlouli et al (2021) it is possible to obtain a production of 322.8 g of second-generation bioethanol from 1 kg of argan pulp through enzymatic hydrolysis pretreatment. This process is economically and environmentally interesting as it produces affordable clean energy and, at the same time, valorize an agricultural waste, contributing to potentially increase the incomes of local farmers, considering that the production of argan pulp is estimated in about 215 kg/ha/year, corresponding to an industrial production of 69.4 kg of bioethanol/ha/year.…”

A Comparative Study of Various Pretreatment Approaches for Bio-Ethanol Production from Willow Sawdust, Using Co-Cultures and Mono-Cultures of Different Yeast Strains

“…Meanwhile, the ethanol yield in terms of g/g carbohydrates was estimated via Equation (7) Ethanol yield = Ethanol (g/100g TS) Initial hollocellulose of WS (g/100g TS) (7)…”

“…In this context, recent progress in the energy sector has focused on the technological conversion of lignocellulosic biomass toward biofuels, including bioethanol [3]. The valorization of agricultural wastes [4][5][6], forestry residues [1,7] and weedy biomass [8] toward second generation bioethanol production [9] has attracted significant attention during the previous decades, aiming to provide a sustainable solution for the reduction of energy dependence on fossil-based fuels, without being antagonistic to food/feed resources and the environmental problems associated with the reliance on them [10].…”