Optimization of Bioethanol Production from Cassava through Fermentation Stimulant with Addition of Alpha-Amylase Enzyme

Abstract

Bioethanol, as a substitute for fuel, presents significant advantages such as environmentally friendly exhaust emissions, high octane value, and a reduction in the use of hazardous additives. The utilization of carbohydrate-rich alternatives as bioethanol feedstock is gaining prominence, particularly root vegetables like cassava. The objective of this research is to obtain bioethanol from cassava through a fermentation stimulant process involving cellulase enzyme and Saccharomyces caravisiase. In the long term, the overarching goal is to establish an alternative energy source with bioethanol production technology derived from cassava. This study comprises three treatments, incorporating the addition of alpha-amylase enzyme at 0.5, 0.1, and 0.15 mL, with a fermentation duration of 56 hours. The research findings reveal that the addition of 0.5 mL of alpha-amylase enzyme produces optimal bioethanol with the highest yield. These results not only underscore the potential of cassava as a primary bioethanol feedstock but also make a significant contribution to the development of efficient and sustainable bioethanol production technology.