Optimization of bioconversion conditions for vitamin D<sub>3</sub>to 25-hydroxyvitamin D using<i>Pseudonocardia autotrophica</i>CGMCC5098

Luo, Jinqi; Jiang, Fang; Fang, Wenhao; Lü, Qun

doi:10.1080/10242422.2016.1268130

Cited by 8 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several prior research studies have applied fermenters of various sizes to study the microbial conversion of vitamin D 3 into its hydroxylated derivatives (Kang et al 2006 , 2015a , b ; Abass et al 2011a , b ; Luo et al 2016 ; Tang et al 2020 ; Wang et al 2022 ). A previous study conducted by Sasaki et al 1991 , where 300 Streptomyces isolates were screened, found two strains with a conversion productivity of about 7.0 mg/L/min.…”

Section: Discussionmentioning

confidence: 99%

Bioconversion of vitamin D3 into calcitriol by Actinomyces hyovaginalis isolate CCASU- A11-2

et al. 2023

View full text Add to dashboard Cite

Vitamin D3 is a fat-soluble prohormone that is activated inside the liver to produce 25-hydroxyvitamin D3 (calcidiol), and in the kidney to produce the fully active 1α, 25-dihydroxy vitamin D3 (calcitriol). A previous work piloted in our laboratory, resulted in a successful recovery of a local soil-promising Actinomyces hyovaginalis isolate CCASU-A11-2 capable of converting vitamin D3 into calcitriol. Despite the rising amount of research on vitamin D3 bioconversion into calcitriol, further deliberate studies on this topic can significantly contribute to the improvement of such a bioconversion process. Therefore, this work aimed to improve the bioconversion process, using the study isolate, in a 14 L laboratory fermenter (4 L fermentation medium composed of fructose (15 g/L), defatted soybean (15 g/L), NaCl (5 g/L), CaCO3 2 g/L); K2HPO4, (1 g/L) NaF (0.5 g/L) and initial of pH 7.8) where different experiments were undertaken to investigate the effect of different culture conditions on the bioconversion process. Using the 14 L laboratory fermenter, the calcitriol production was increased by about 2.5-fold (32.8 µg/100 mL) to that obtained in the shake flask (12.4 µg/100 mL). The optimal bioconversion conditions were inoculum size of 2% v/v, agitation rate of 200 rpm, aeration rate of 1 vvm, initial pH of 7.8 (uncontrolled); addition of vitamin D3 (substrate) 48 h after the start of the main culture. In conclusion, the bioconversion of vitamin D3 into calcitriol in a laboratory fermenter showed a 2.5-fold increase as compared to the shake flask level where, the important factors influencing the bioconversion process were the aeration rate, inoculum size, the timing of substrate addition, and the fixed pH of the fermentation medium. So, those factors should be critically considered for the scaling-up of the biotransformation process.

show abstract

Section: Discussionmentioning

confidence: 99%

Bioconversion of vitamin D3 into calcitriol by Actinomyces hyovaginalis isolate CCASU- A11-2

et al. 2023

View full text Add to dashboard Cite

show abstract

“…It is known that Pseudonocardia Gsoil 1536 converts Re to Rg2(S) (Du et al, 2014). Besides, vitamin D3 hydroxylase (Vdh) is produced by P. autotrophica CGMCC5098, which converts 25(OH)VD 3 to 1a,25(OH) VD 3 (Luo et al, 2017). Furthermore, 1a,25(OH)VD3 is an active form of VD3 with efficacy in the treatment of some diseases, such as chronic renal failure, hyperparathyroidism, osteoporosis and psoriasis (Kang et al, 2015).…”

Section: Other Secondary Metabolitesmentioning

confidence: 99%

mentioning

confidence: 99%

Genus Pseudonocardia : What we know about its biological properties, abilities and current application in biotechnology

Riahi

Heidarieh

Bafghi

2021

J of Applied Microbiology

View full text Add to dashboard Cite

The genus Pseudonocardia belongs to a group of actinomycetes, and is a member of the family Pseudonocardiacea. The members of this genus are aerobic, Gram-positive, non-motile bacteria that are commonly found in soil, plant, and environment. Although this genus has a low clinical significance, however it has an important role in biotechnology due to the production of secondary metabolites, some of which have anti-bacterial, anti-fungal, and anti-tumor effects. The use of phenotypic tests such as gelatinase activity, starch hydrolysis, catalase, and oxidase tests, as well as molecular methods, such as polymerase chain reaction (PCR), are necessary for Pseudonocardia identification at the genus and species levels.

show abstract

Investigation of Vitamin D₂ and Vitamin D₃ Hydroxylation by Kutzneria albida

et al. 2021

View full text Add to dashboard Cite

The active vitamin D metabolites 25‐OH−D and 1α,25‐(OH)2−D play an essential role in controlling several cellular processes in the human body and are potentially effective in the treatment of several diseases, such as autoimmune diseases, cardiovascular diseases and cancer. The microbial synthesis of vitamin D2 (VD2) and vitamin D3 (VD3) metabolites has emerged as a suitable alternative to established complex chemical syntheses. In this study, a novel strain, Kutzneria albida, with the ability to form 25‐OH−D2 and 25‐OH−D3 was identified. To further improve the conversion of the poorly soluble substrates, several solubilizers were tested. 100‐fold higher product concentrations of 25‐OH−D3 and tenfold higher concentrations of 25‐OH−D2 after addition of 5 % (w/v) 2‐hydroxypropyl β‐cyclodextrin (2‐HPβCD) were reached. Besides the single‐hydroxylation products, the human double‐hydroxylation products 1,25‐(OH)2−D2 and 1,25‐(OH)2−D3 and various other potential single‐ and double‐hydroxylation products were detected. Thus, K. albida represents a promising strain for the biotechnological production of VD2 and VD3 metabolites.

show abstract

Optimization of bioconversion conditions for vitamin D₃to 25-hydroxyvitamin D usingPseudonocardia autotrophicaCGMCC5098

Cited by 8 publications

References 16 publications

Bioconversion of vitamin D3 into calcitriol by Actinomyces hyovaginalis isolate CCASU- A11-2

Bioconversion of vitamin D3 into calcitriol by Actinomyces hyovaginalis isolate CCASU- A11-2

Genus Pseudonocardia : What we know about its biological properties, abilities and current application in biotechnology

Investigation of Vitamin D₂ and Vitamin D₃ Hydroxylation by Kutzneria albida

Contact Info

Product

Resources

About

Optimization of bioconversion conditions for vitamin D3to 25-hydroxyvitamin D usingPseudonocardia autotrophicaCGMCC5098

Cited by 8 publications

References 16 publications

Bioconversion of vitamin D3 into calcitriol by Actinomyces hyovaginalis isolate CCASU- A11-2

Bioconversion of vitamin D3 into calcitriol by Actinomyces hyovaginalis isolate CCASU- A11-2

Genus Pseudonocardia : What we know about its biological properties, abilities and current application in biotechnology

Investigation of Vitamin D2 and Vitamin D3 Hydroxylation by Kutzneria albida

Contact Info

Product

Resources

About

Optimization of bioconversion conditions for vitamin D₃to 25-hydroxyvitamin D usingPseudonocardia autotrophicaCGMCC5098

Investigation of Vitamin D₂ and Vitamin D₃ Hydroxylation by Kutzneria albida