Optimization of antioxidant extraction from edible brown algae Ascophyllum nodosum using response surface methodology

Liu, Xin; Luo, Guanghong; Wang, Limin; Yuan, Wenqiao

doi:10.1016/j.fbp.2019.01.003

Cited by 28 publications

(23 citation statements)

References 33 publications

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“…The same authors reported a reduction in the yields of compounds extracted when using ethanol concentrations above 50% due to an increased extraction of other less polar components. Another study on optimisation of the extraction variables on the yields of crude extracts from Ascophyllum nodosum, also observed that ethanol concentration had a significant effect on the yield of extract obtained, and that lower ethanol concentrations enhanced the extraction yield, suggesting that most of the extractable compounds were high in polarity [19]. Table 1 summarises the recovery of total polyphenols and phlorotannins extracted as well as retained (unextracted) in the residue of F. vesiculosus seaweed after ultrasound treatment.…”

Section: Effect Of Ultrasound On Extraction Yield Total Polyphenols mentioning

confidence: 92%

Optimisation of Ultrasound Frequency, Extraction Time and Solvent for the Recovery of Polyphenols, Phlorotannins and Associated Antioxidant Activity from Brown Seaweeds

et al. 2020

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This study investigates ultrasound assisted extraction (UAE) process parameters (time, frequency and solvent) to obtain high yields of phlorotannins, flavonoids, total phenolics and associated antioxidant activities from 11 brown seaweed species. Optimised UAE conditions (35 kHz, 30 min and 50% ethanol) significantly improved the extraction yield from 1.5-fold to 2.2-fold in all seaweeds investigated compared to solvent extraction. Using ultrasound, the highest recovery of total phenolics (TPC: 572.3 ± 3.2 mg gallic acid equivalent/g), total phlorotannins (TPhC: 476.3 ± 2.2 mg phloroglucinol equivalent/g) and total flavonoids (TFC: 281.0 ± 1.7 mg quercetin equivalent/g) was obtained from Fucus vesiculosus seaweed. While the lowest recovery of TPC (72.6 ± 2.9 mg GAE/g), TPhC (50.3 ± 2.0 mg PGE/g) and TFC (15.2 ± 3.3 mg QE/g) was obtained from Laminaria digitata seaweed. However, extracts from Fucus serratus obtained by UAE exhibited the strongest 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity (29.1 ± 0.25 mg trolox equivalent/g) and ferric reducing antioxidant power (FRAP) value (63.9 ± 0.74 mg trolox equivalent/g). UAE under optimised conditions was an effective, low-cost and eco-friendly technique to recover biologically active polyphenols from 11 brown seaweed species.

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Section: Effect Of Ultrasound On Extraction Yield Total Polyphenols mentioning

confidence: 92%

Optimisation of Ultrasound Frequency, Extraction Time and Solvent for the Recovery of Polyphenols, Phlorotannins and Associated Antioxidant Activity from Brown Seaweeds

et al. 2020

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“…Another hypothesis is that the pressurization of PLE may affect the protein structure, resulting in more small-molecule peptides. Moreover, some previous studies have shown that pressurization can affect the structure of protein molecules in food, that is, the pressures below 150 MPa can affect the quaternary structure of proteins; 200 MPa can affect the tertiary structure, and 300–700 MPa can change the secondary structure [ 36 , 37 ]. Obviously, the pressure in our study is close to 10 MPa, which is not enough to change any structure other than the quaternary structure of the protein.…”

Section: Resultsmentioning

confidence: 99%

Impact of Pressurized Liquid Extraction and pH on Protein Yield, Changes in Molecular Size Distribution and Antioxidant Compounds Recovery from Spirulina

Zhou

Wang

Carrillo

et al. 2021

Foods

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The research aims to extract nutrients and bioactive compounds from spirulina using a non-toxic, environmentally friendly and efficient method—Pressurized Liquid Extraction (PLE). In this work, Response Surface Methodology (RSM)–Central Composite Design (CCD) was used to evaluate and optimize the extraction time (5–15 min), temperature (20–60 °C) and pH (4–10) during PLE extraction (103.4 bars). The multi-factor optimization results of the RSM-CCD showed that under the pressure of 103.4 bars, the optimal conditions to recover the highest content of bioactive compounds were 10 min, 40 °C and pH 4. Furthermore, the compounds and antioxidant capacity of PLE and non-pressurized extraction extracts were compared. The results showed that under the optimal extraction conditions (10 min, 40 °C and pH 4), PLE significantly improved the antioxidant capacity (2870.5 ± 153.6 µM TE), protein yield (46.8 ± 3.1%), chlorophyll a (1.46 ± 0.04 mg/g), carotenoids (0.12 ± 0.01 mg/g), total polyphenols (11.49 ± 0.04 mg/g) and carbohydrates content (78.42 ± 1.40 mg/g) of the extracts compared with non-pressurized extraction (p < 0.05). The protein molecular distribution of the extracts was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and the results showed that there were more small-molecule proteins in PLE extracts. Moreover, Liquid Chromatography Triple Time of Flight Mass Spectrometry (TOF–LC–MS–MS) was used to analyze the phenolic profile of the extracts, and the results showed the extracts were rich on phenolic compounds, such as p-coumaric acid and cinnamic acid being the predominant phenolic compounds in the PLE extract. This indicates that PLE can promote the extraction of bioactive compounds from Spirulina, which is of great significance for the application of PLE technology to obtain active substances from marine algae resources.

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“…The factorial design obtained by RSM allows the effects of multiple-variables and also their interaction to be evaluated simultaneously 29,30 . The factorial design includes three major steps: (1) set the independent variables and responses, in which the number of variables and their levels is decided from a preliminary experiment; (2) interpretation of response surface modelling using contour (2D) and surface plot (3D) interaction among the variables; (3) model validationn 31 . Different factorial designs can be performed such as Box-Behnken or Central Composite design.…”

Section: Introductionmentioning

confidence: 99%

“…The Box-Behnken design has better data accuracy, is more cost-effective and considerably less experimental runs care required ompared to the classical approach 16,31 . Various types of sample matrix can be applied in the Box-Behnken experimental design for extraction of the phenolic compounds such as samara oil 32 , algae 31 , glutinous rice bran 33 , cherry fruits 24 , Ilex guayusa Loes. leaves 34 and Citrus medica 16 .…”

Section: Introductionmentioning

confidence: 99%

Ultrasonic-Assisted Extraction (UAE) for Enhanced Recovery of Bioactive Phenolic Compounds From Cosmos Caudatus Leaves

Latiff

Ong

Abdullah

et al. 2021

Preprint

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Cosmos caudatus (C. caudatus) is one of the common medicinal plants and among the valuable plants that are high in bioactive compounds such as phenolics. In this study, an ultrasound-assisted extraction (UAE) method was used to optimise the extraction of bioactive compounds from C. caudatus leaves using response surface methodology (RSM) and employing a Box-Behnken experimental design (BBD). The extraction efficiency of UAE under the optimal extraction conditions was compared with the Soxhlet method. Also, an anti-microbial analysis against two human pathogenic bacteria; Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) was also evaluated. The effect of the extraction conditions was studied and optimised such as the solid-liquid ratio (10 to 30 g/ml), particle size (180 to 850 µm) and extraction time (20 to 30 min). Quercitrin and total phenolic content (TPC) were the selected response variables in this study. Based on the ANOVA analysis, the response surface model to predict the optimum yield of quercitrin and TPC was adequate with a high R-square value corresponding to 0.9930 and 0.9962. The optimal UAE conditions were 1:28 (g/mL), by using a particle size of 485 µm and an extraction time of 30 min, respectively. Remarkably, UAE reduced the extraction time and solvent volume, with the maximal recovery of bioactive compounds at a high antioxidant activity as compared with that of the Soxhlet method. The plant extract also exhibited potential microbial agents. Due to the above findings, UAE can be used to enrich quercitrin and total phenolic content from C. caudatus leaves. It also opens the possibility of plant extract to be used as an affordable component in many applications such as food formulations and anti-microbial agents.

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Optimization of antioxidant extraction from edible brown algae Ascophyllum nodosum using response surface methodology

Cited by 28 publications

References 33 publications

Optimisation of Ultrasound Frequency, Extraction Time and Solvent for the Recovery of Polyphenols, Phlorotannins and Associated Antioxidant Activity from Brown Seaweeds

Optimisation of Ultrasound Frequency, Extraction Time and Solvent for the Recovery of Polyphenols, Phlorotannins and Associated Antioxidant Activity from Brown Seaweeds

Impact of Pressurized Liquid Extraction and pH on Protein Yield, Changes in Molecular Size Distribution and Antioxidant Compounds Recovery from Spirulina

Ultrasonic-Assisted Extraction (UAE) for Enhanced Recovery of Bioactive Phenolic Compounds From Cosmos Caudatus Leaves

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