“…In line with Li et al [13], the continuous anaerobic digestion experiment involved the stepwise loading of the reactors of the digester with prepared substrates of four different organic loading rates (OLRs) of 1, 2.5, 3.5, and 4.5 gVS/L/d and was operated for over 120 days. Organic loading rate, also known as volume load means the amount of organic dry solids loaded per liter volume of digester reactor per unit time, which can be delivered without overfeeding the bacteria, which could lead to process stress [5]. The continuous anaerobic digestion experiment got to steady state within 20, 9, 6, 3 days for OLRs 1, 2.5, 3.5, and 4.5 gVS/L/d, respectively.…”