Optimization of an enzyme-coupling method by spectrophotometer for serum adenosine deaminase: As a candidate reference method

Han, Liqiao; Huang, Xiaoting; Wang, Jianbing; Lin, Haibiao; Zhang, Qiaoxuan; Gu, Yongmei; Sun, Keqi; Yang, Yongdan; Yan, Jun; Ke, Peifeng; Huang, Xianzhang; Zhang, Junhua

doi:10.1016/j.ab.2019.113462

Cited by 1 publication

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ADA was determined by the peroxidase method. 21 Total protein was determined by the biuret method. LDH, ADA, and glucose were determined by a Beckman AU5800 biochemical analyzer.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Pleural homocysteine for malignant pleural effusion: A prospective and double‐blind diagnostic test accuracy study

et al. 2022

View full text Add to dashboard Cite

Objective To assess the accuracy of pleural fluid homocysteine for discriminating malignant pleural effusion (MPE) and benign pleural effusion (BPE). Methods A total of 194 patients from two cohorts (Hohhot and Changshu) with undiagnosed pleural effusion were prospectively enrolled. Their pleural homocysteine was measured, and its diagnostic accuracy and net benefit for MPE were analyzed by receiver operating characteristic (ROC) curve analysis and decision curve analysis, respectively. Results In the Hohhot cohort ( n = 136) and the Changshu cohort ( n = 58), MPE patients had significantly higher homocysteine levels than BPE patients. The areas under the ROC curves of homocysteine for the diagnosis of MPE were 0.61 ( p = 0.027) and 0.59 ( p = 0.247), respectively. The decision curves of homocysteine were close to the reference line in both the Hohhot cohort and the Changshu cohort. Conclusion The diagnostic accuracy of pleural fluid homocysteine for MPE was low.

show abstract

“…ADA was determined by the peroxidase method. 21 Total protein was determined by the biuret method. LDH, ADA, and glucose were determined by a Beckman AU5800 biochemical analyzer.…”

Section: Methodsmentioning

confidence: 99%

“…LDH was determined by the lactate‐to‐pyruvate method. ADA was determined by the peroxidase method 21 . Total protein was determined by the biuret method.…”

Section: Methodsmentioning

confidence: 99%