Optimization of an enriched mixed culture to increase PHA accumulation using industrial saline complex wastewater as a substrate

Argiz, Lucía; Fra-Vázquez, Andrea; Río, Ángeles Val del; Mosquera-Corral, Anuska

doi:10.1016/j.chemosphere.2020.125873

Cited by 38 publications

(20 citation statements)

References 31 publications

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“…The feasibility of the MMC-based processes on waste substrates has been demonstrated at pilot scale, and several European projects aim at demonstrating PHA production at pilot scale have been funded (Mannina et al, 2020). Future improvements for the exploitation of MMC-based processes should address the increase in cell densities and polymer productivities (Argiz et al, 2020) as well as the improvements in the extraction technologies due to their higher resistance to cell hydrolysis with respect to pure cultures (Samorì et al, 2015;Mannina et al, 2020).…”

Section: Challenges and Perspectives For Effective Pha Exploitationmentioning

confidence: 99%

In vivo and Post-synthesis Strategies to Enhance the Properties of PHB-Based Materials: A Review

Turco

Santagata

Corrado

et al. 2021

Front. Bioeng. Biotechnol.

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The transition toward “green” alternatives to petroleum-based plastics is driven by the need for “drop-in” replacement materials able to combine characteristics of existing plastics with biodegradability and renewability features. Promising alternatives are the polyhydroxyalkanoates (PHAs), microbial biodegradable polyesters produced by a wide range of microorganisms as carbon, energy, and redox storage material, displaying properties very close to fossil-fuel-derived polyolefins. Among PHAs, polyhydroxybutyrate (PHB) is by far the most well-studied polymer. PHB is a thermoplastic polyester, with very narrow processability window, due to very low resistance to thermal degradation. Since the melting temperature of PHB is around 170–180°C, the processing temperature should be at least 180–190°C. The thermal degradation of PHB at these temperatures proceeds very quickly, causing a rapid decrease in its molecular weight. Moreover, due to its high crystallinity, PHB is stiff and brittle resulting in very poor mechanical properties with low extension at break, which limits its range of application. A further limit to the effective exploitation of these polymers is related to their production costs, which is mostly affected by the costs of the starting feedstocks. Since the first identification of PHB, researchers have faced these issues, and several strategies to improve the processability and reduce brittleness of this polymer have been developed. These approaches range from the in vivo synthesis of PHA copolymers, to the enhancement of post-synthesis PHB-based material performances, thus the addition of additives and plasticizers, acting on the crystallization process as well as on polymer glass transition temperature. In addition, reactive polymer blending with other bio-based polymers represents a versatile approach to modulate polymer properties while preserving its biodegradability. This review examines the state of the art of PHA processing, shedding light on the green and cost-effective tailored strategies aimed at modulating and optimizing polymer performances. Pioneering examples in this field will be examined, and prospects and challenges for their exploitation will be presented. Furthermore, since the establishment of a PHA-based industry passes through the designing of cost-competitive production processes, this review will inspect reported examples assessing this economic aspect, examining the most recent progresses toward process sustainability.

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Section: Challenges and Perspectives For Effective Pha Exploitationmentioning

confidence: 99%

In vivo and Post-synthesis Strategies to Enhance the Properties of PHB-Based Materials: A Review

Turco

Santagata

Corrado

et al. 2021

Front. Bioeng. Biotechnol.

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“…To enhance PHA accumulation from saline wastewater the implementation of a settling stage after the feast phase in the enrichment unit was assessed. An increase from 40 to 60% of maximum PHA accumulation was observed, along with an effect on the co-polymer composition, and HV proportion rose from 17 to 30% (Argiz et al, 2020).…”

Section: Treatment and Valorisation Of Saline Wastewater 104mentioning

confidence: 83%

“…The production of PHA in halophilic conditions has been studied extensively in systems based on pure cultures (Quillaguamán et al, 2010). Nevertheless, information is scarce regarding PHA obtainment from saline effluents and with non-pure cultures (Argiz et al, 2020;Palmeiro-Sánchez et al, 2016a, 2016b) (Table 5.2). The use of halophilic mixed cultures presents several advantages.…”

Section: Polyhydroxyalkanoates (Pha)mentioning

confidence: 99%

Treatment and Valorisation of Saline Wastewater: Principles and Practice

Río¹,

Corral²

2021

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Salinisation of freshwater occurs around the world due to anthropogenic activities associated with urban and industrial activities. These activities include groundwater abstraction for potable water supply to levels that favour seawater intrusions in coastal areas, the use of decalcifying products to prevent damage to appliances and the utilisation of salt media in industrial processes. These uses of water produce saline wastewater which is subjected to cleaning treatments that do not include salt removal. Thus, treated wastewater is reintroduced to the environment with salt levels that reduce its quality and make its further utilisation difficult. For this reason, an evaluation of the sources of wastewater with salt concentrations (e.g. NaCl) in the range from 1,300 (moderately saline) to 28,800 mg/L (very highly saline) will be provided in this section. Characteristics and compositions will be described for urban and industrial wastewater. The specific problems associated with the presence of salts will be presented and discussed.

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“…The maximum PHA storage capacity (PHA ~ 40-60 wt%) was reported using mixed cultures in an SBR operated in cycles of feast/famine phases. Thus, during the settling step, i.e., after the feast phase, the washout of non-PHA storing bacterial cells was achieved [110]. PHA accumulation occurred at higher substrate loads (40% of the weight of the dry cell, DCW), low nitrogen (45% DCW), and low phosphorus (54% DCW) conditions [111].…”

Section: Potential For the Recovery Of Nutrients And Phamentioning

confidence: 99%

Removal and recovery of nutrients and value-added products from wastewater: technological options and practical perspective

Srivastava

Pothu

Sanchez

et al. 2021

Syst Microbiol and Biomanuf

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Wastewaters from various process industries, namely food and agricultural, sugar mill, brewery, milk, vegetable and fruit, and meat and fisheries processing industries and their wastewater effluents contain nutrients, organic matter, inorganic, heavy metals, suspended solids, and pathogens. The discharges of non-treated wastewater enter the nearby aquatic ecosystem (e.g., lakes, rivers) and are a significant concern due to the presence of different nutrients, competing ions and C containing pollutants. It causes excessive growth of algae, loss of habitat/species, and other negative impacts on human health/environment. In the present review, different treatment approaches have been discussed in utilizing these nutrients to synthesize value-added products such as biopolymer, biofuel, pigment, organic acid, or enzymes. These biopolymers can be used to prepare various food products/packaging materials. Dextran, chitosan, carrageenan, alginate, and pectin are good examples of non-food biopolymers. Besides these products, poly-β-hydroxybutyrate (PHB) synthesis from wastewater nutrients is reported as a new source of bio-nanocomposite materials/biopolymer-based coatings. In this review, the different treatment approaches are discussed, which are being used worldwide for the removal/recovery of nutrients, toxic pollutants, and the potential resource recovery of value-added products from wastewater.

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Optimization of an enriched mixed culture to increase PHA accumulation using industrial saline complex wastewater as a substrate

Cited by 38 publications

References 31 publications

In vivo and Post-synthesis Strategies to Enhance the Properties of PHB-Based Materials: A Review

In vivo and Post-synthesis Strategies to Enhance the Properties of PHB-Based Materials: A Review

Treatment and Valorisation of Saline Wastewater: Principles and Practice

Removal and recovery of nutrients and value-added products from wastewater: technological options and practical perspective

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