Optimization of an Autochthonous Bacterial Consortium Obtained from Beach Sediments for Bioremediation of Petroleum Hydrocarbons

Perdigão, Rafaela; Almeida, C. Marisa R.; Santos, Flávia Carvalho; Carvalho, Maria de Fátima; Mucha, Ana P.

doi:10.3390/w13010066

Cited by 22 publications

(18 citation statements)

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“…We found the lowest hydrocarbons degradation in natural attenuation (18%), followed by biostimulation treatment (21%), bioaugmentation with inoculum pre-grown in petroleum treatment (23%), and bioaugmentation with inoculum pre-grown in acetate treatment (30%). The same trend for higher degradation in the BAa (bioaugmentation treatment with inoculum pre-grown in sodium acetate), when compared to BAp (bioaugmentation treatments with inoculum pre-grown petroleum), has been observed in a previous study [37], indicating that this result was associated with the higher bacterial biomass growth obtained in the presence of acetate compared with petroleum.…”

Section: Discussionsupporting

confidence: 82%

“…Initially, two different carbon sources, petroleum and sodium acetate, were tested to compare their efficiency to produce high consortium biomass. Being a simple carbon source, sodium acetate is rapidly uptake by most bacteria and had been already tested in previous biodegradation experiments [37]. Our results showed that the HDB_DSS_L1 bacterial consortium grown on sodium acetate was able to survive and grow in petroleum in the bioaugmentation treatment.…”

Section: Discussionmentioning

confidence: 59%

“…Bacterial strains isolated from the L1 site were selected to assemble the Deep-Sea Sediment Hydrocarbons-Degrading Bacterial consortium (HDB_L1_DSS) to be used in the bioremediation experiment. For this, two inocula were prepared, one with petroleum and another with sodium acetate, based on a previous growth optimization experiment [37]. For inoculum preparation, a mixture of the eight bacterial strains were added to 250 mL sterilized glass flasks containing 20 mL BH medium supplemented with 2% NaCl, with an OD600 nm of 1.…”

Section: Microcosm Bioremediation Experimentsmentioning

confidence: 99%

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Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean)

et al. 2021

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Deep-sea sediments (DSS) are one of the largest biotopes on Earth and host a surprisingly diverse microbial community. The harsh conditions of this cold environment lower the rate of natural attenuation, allowing the petroleum pollutants to persist for a long time in deep marine sediments raising problematic environmental concerns. The present work aims to contribute to the study of DSS microbial resources as biotechnological tools for bioremediation of petroleum hydrocarbon polluted environments. Four deep-sea sediment samples were collected in the Mid-Atlantic Ridge, south of the Azores (North Atlantic Ocean). Their autochthonous microbial diversity was investigated by 16S rRNA metabarcoding analysis. In addition, a total of 26 deep-sea bacteria strains with the ability to utilize crude oil as their sole carbon and energy source were isolated from the DSS samples. Eight of them were selected for a novel hydrocarbonoclastic-bacterial consortium and their potential to degrade petroleum hydrocarbons was tested in a bioremediation experiment. Bioaugmentation treatments (with inoculum pre-grown either in sodium acetate or petroleum) showed an increase in degradation of the hydrocarbons comparatively to natural attenuation. Our results provide new insights into deep-ocean oil spill bioremediation by applying DSS hydrocarbon-degrading consortium in lab-scale microcosm to simulate an oil spill in natural seawater.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 59%

Section: Microcosm Bioremediation Experimentsmentioning

confidence: 99%

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Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean)

et al. 2021

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“…Despite the increase of recent studies in bioremediation of petroleum HCs (Chen et al, 2020;Shi et al, 2020;Perdigão et al, 2021), there is still a lack of knowledge regarding the natural repertoire of the microbial communities able to degrade petroleum HCs. This study addresses the geospatial variation of these natural microbial communities in NW coast of the Iberian Peninsula to identify prokaryotic groups with potential bioremediation applicability in this region.…”

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confidence: 99%

Harnessing the Potential of Native Microbial Communities for Bioremediation of Oil Spills in the Iberian Peninsula NW Coast

et al. 2021

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Oil spills are among the most catastrophic events to marine ecosystems and current remediation techniques are not suitable for ecological restoration. Bioremediation approaches can take advantage of the activity of microorganisms with biodegradation capacity thus helping to accelerate the recovery of contaminated environments. The use of native microorganisms can increase the bioremediation efficiency since they have higher potential to survive in the natural environment while preventing unpredictable ecological impacts associated with the introduction of non-native organisms. In order to know the geographical scale to which a native bioremediation consortium can be applied, we need to understand the spatial heterogeneity of the natural microbial communities with potential for hydrocarbon degradation. In the present study, we aim to describe the genetic diversity and the potential of native microbial communities to degrade petroleum hydrocarbons, at an early stage of bioremediation, along the NW Iberian Peninsula coast, an area particularly susceptible to oil spills. Seawater samples collected in 47 sites were exposed to crude oil for 2 weeks, in enrichment experiments. Seawater samples collected in situ, and samples collected after the enrichment with crude oil, were characterized for prokaryotic communities by using 16S rRNA gene amplicon sequencing and predictive functional profiling. Results showed a drastic decrease in richness and diversity of microbial communities after the enrichment with crude oil. Enriched microbial communities were mainly dominated by genera known to degrade hydrocarbons, namely Alcanivorax, Pseudomonas, Acinetobacter, Rhodococcus, Flavobacterium, Oleibacter, Marinobacter, and Thalassospira, without significant differences between geographic areas and locations. Predictive functional profiling of the enriched microbial consortia showed a high potential to degrade the aromatic compounds aminobenzoate, benzoate, chlorocyclohexane, chlorobenzene, ethylbenzene, naphthalene, polycyclic aromatic compounds, styrene, toluene, and xylene. Only a few genera contributed for more than 50% of this genetic potential for aromatic compounds degradation in the enriched communities, namely Alcanivorax, Thalassospira, and Pseudomonas spp. This work is a starting point for the future development of prototype consortia of hydrocarbon-degrading bacteria to mitigate oil spills in the Iberian NW coast.

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“…In situ and laboratory studies reported that the biodegradation efficiency of petroleum-contaminated sediments can be increased by enhancing biomass and/or activity of hydrocarbon-degrading microorganisms through biostimulation as well as bioaugmentation strategies [ 44 ]. Understanding the factors influencing microbial metabolism and hydrocarbon degradation is crucial to the design of an optimal bioremediation strategy [ 45 , 46 , 47 ].…”

Section: Factors Affecting the Bioremediation Of Sediments Polluted By Petroleum Hydrocarbonsmentioning

confidence: 99%

Bacteria, Fungi and Microalgae for the Bioremediation of Marine Sediments Contaminated by Petroleum Hydrocarbons in the Omics Era

et al. 2021

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Petroleum hydrocarbons (PHCs) are one of the most widespread and heterogeneous organic contaminants affecting marine ecosystems. The contamination of marine sediments or coastal areas by PHCs represents a major threat for the ecosystem and human health, calling for urgent, effective, and sustainable remediation solutions. Aside from some physical and chemical treatments that have been established over the years for marine sediment reclamation, bioremediation approaches based on the use of microorganisms are gaining increasing attention for their eco-compatibility, and lower costs. In this work, we review current knowledge concerning the bioremediation of PHCs in marine systems, presenting a synthesis of the most effective microbial taxa (i.e., bacteria, fungi, and microalgae) identified so far for hydrocarbon removal. We also discuss the challenges offered by innovative molecular approaches for the design of effective reclamation strategies based on these three microbial components of marine sediments contaminated by hydrocarbons.

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Optimization of an Autochthonous Bacterial Consortium Obtained from Beach Sediments for Bioremediation of Petroleum Hydrocarbons

Cited by 22 publications

References 62 publications

Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean)

Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean)

Harnessing the Potential of Native Microbial Communities for Bioremediation of Oil Spills in the Iberian Peninsula NW Coast

Bacteria, Fungi and Microalgae for the Bioremediation of Marine Sediments Contaminated by Petroleum Hydrocarbons in the Omics Era

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