Optimization of alginate purification using polyvinylidene difluoride membrane filtration: Effects on immunogenicity and biocompatibility of three-dimensional alginate scaffolds

Sondermeijer, Hugo; Witkowski, Piotr; Woodland, David C.; Seki, Tetsunori; Aangenendt, Frank J.; Laarse, Arnoud van der; Itescu, Silviu; Hardy, Mark A.

doi:10.1177/0885328216645952

Cited by 31 publications

(16 citation statements)

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“…Common alginate contaminants include proteins, polyphenolic compounds, lipopolysaccharides (endotoxins) and genetic material (DNA and RNA), some of which may trigger undesirable host responses. Several works have compared the performance of purified vs. unpurified alginates regarding biological properties, particularly the possibility of eliciting undesirable immunological response (Dusseault et al, 2006 ; Tam et al, 2006 ; Sondermeijer et al, 2016 ; Torres et al, 2019 ). Despite the much lower levels of contaminants in purified alginates comparing to unpurified ones (Sondermeijer et al, 2016 ), purification is still one of the roadblocks for translational application of alginate-based products to the clinics (Krishnan et al, 2017 ).…”

Section: Overview Of Alginate As Biomaterialsmentioning

confidence: 99%

“…Several works have compared the performance of purified vs. unpurified alginates regarding biological properties, particularly the possibility of eliciting undesirable immunological response (Dusseault et al, 2006 ; Tam et al, 2006 ; Sondermeijer et al, 2016 ; Torres et al, 2019 ). Despite the much lower levels of contaminants in purified alginates comparing to unpurified ones (Sondermeijer et al, 2016 ), purification is still one of the roadblocks for translational application of alginate-based products to the clinics (Krishnan et al, 2017 ). Fortunately, alginates of ultrapure grade are already commercially available, with well-defined compositions (i.e., G/M ratio, molecular weight/viscosity), and can be even acquired as sterilized products, if desired (Bidarra et al, 2014 ).…”

Section: Overview Of Alginate As Biomaterialsmentioning

confidence: 99%

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Modulating Alginate Hydrogels for Improved Biological Performance as Cellular 3D Microenvironments

Neves

Moroni

Barrias

2020

Front. Bioeng. Biotechnol.

131

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The rational choice and design of biomaterials for biomedical applications is crucial for successful in vitro and in vivo strategies, ultimately dictating their performance and potential clinical applications. Alginate, a marine-derived polysaccharide obtained from seaweeds, is one of the most widely used polymers in the biomedical field, particularly to build three dimensional (3D) systems for in vitro culture and in vivo delivery of cells. Despite their biocompatibility, alginate hydrogels often require modifications to improve their biological activity, namely via inclusion of mammalian cell-interactive domains and fine-tuning of mechanical properties. These modifications enable the addition of new features for greater versatility and control over alginate-based systems, extending the plethora of applications and procedures where they can be used. Additionally, hybrid systems based on alginate combination with other components can also be explored to improve the mimicry of extracellular microenvironments and their dynamics. This review provides an overview on alginate properties and current clinical applications, along with different strategies that have been reported to improve alginate hydrogels performance as 3D matrices and 4D dynamic systems.

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Section: Overview Of Alginate As Biomaterialsmentioning

confidence: 99%

Section: Overview Of Alginate As Biomaterialsmentioning

confidence: 99%

Modulating Alginate Hydrogels for Improved Biological Performance as Cellular 3D Microenvironments

Neves

Moroni

Barrias

2020

Front. Bioeng. Biotechnol.

131

View full text Add to dashboard Cite

show abstract

“…Purification is a technique utilized to minimize the amount of contaminations in the alginate gels. Commercially available sodium alginate can be purchased in several different purified states including "ultra-purified" form from companies like Novamatrix ® for use in hydrogel production [94][95][96]. Although this is highly purified there are still small concentrations of contaminants in the alginate that can prompt a severe foreign body response [91,97].…”

Section: Alginate Purity Effect On Immunogenicitymentioning

confidence: 99%

Current Perspective and Advancements of Alginate-Based Transplantation Technologies

Rodriguez¹,

Tuli²,

Wheeler³

et al. 2020

Alginates - Recent Uses of This Natural Polymer

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Versatile yet biocompatible bio-materials are in high demand in nearly every industry, with biological and biomedical engineering relying heavily on common biomaterials like alginate polymers. Alginate is a very common substance found in various marine plants which can easily be extracted and purified through cheap nonhazardous methods. A key characteristic of alginate polymers includes easily manipulatable physical properties due to its inert but functional chemical composition. Factors including its functional versatility, long-term polymer stability and biocompatibility have caused alginate-based technologies to draw major attention from both the scientific and industrial communities alike. While also used in food industry manufacturing and standard dental procedures, this chapter will focus on a discussion of the both clinical and nonclinical use of alginate-based technologies in transplantation for regenerative cell and drug delivery systems. In addition, we overview the immune system response prompted following implantation of alginate hydrogels. Consequences of immune cell reactivity to foreign materials, such as inflammation and the foreign body response (FBR), are also analyzed and current and future strategies for potential circumvention of severe immune responses toward alginate-based devices are reviewed and suggested.

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“…In 2016 a novel purification strategy was added to the list of methods. This method is based on activated charcoal treatment, hydrophobic membrane filtration and dialysis (Sondermeijer et al, 2016). Using this approach, purified alginate was created that induced minimal foreign body reactions up to 1 month after implantation.…”

Section: Attenuate Host Responses By Rational Choices For Polymersmentioning

confidence: 99%

Polymeric Approaches to Reduce Tissue Responses Against Devices Applied for Islet-Cell Encapsulation

Vos

2019

Front. Bioeng. Biotechnol.

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Immunoisolation of pancreatic islets is a technology in which islets are encapsulated in semipermeable but immunoprotective polymeric membranes. The technology allows for successful transplantation of insulin-producing cells in the absence of immunosuppression. Different approaches of immunoisolation are currently under development. These approaches involve intravascular devices that are connected to the bloodstream and extravascular devices that can be distinguished in micro- and macrocapsules and are usually implanted in the peritoneal cavity or under the skin. The technology has been subject of intense fundamental research in the past decade. It has co-evolved with novel replenishable cell sources for cure of diseases such as Type 1 Diabetes Mellitus that need to be protected for the host immune system. Although the devices have shown significant success in animal models and even in human safety studies most technologies still suffer from undesired tissue responses in the host. Here we review the past and current approaches to modulate and reduce tissue responses against extravascular cell-containing micro- and macrocapsules with a focus on rational choices for polymer (combinations). Choices for polymers but also choices for crosslinking agents that induce more stable and biocompatible capsules are discussed. Combining beneficial properties of molecules in diblock polymers or application of these molecules or other anti-biofouling molecules have been reviewed. Emerging are also the principles of polymer brushes that prevent protein and cell-adhesion. Recently also immunomodulating biomaterials that bind to specific immune receptors have entered the field. Several natural and synthetic polymers and even combinations of these polymers have demonstrated significant improvement in outcomes of encapsulated grafts. Adequate polymeric surface properties have been shown to be essential but how the surface should be composed to avoid host responses remains to be identified. Current insight is that optimal biocompatible devices can be created which raises optimism that immunoisolating devices can be created that allows for long term survival of encapsulated replenishable insulin-producing cell sources for treatment of Type 1 Diabetes Mellitus.

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Optimization of alginate purification using polyvinylidene difluoride membrane filtration: Effects on immunogenicity and biocompatibility of three-dimensional alginate scaffolds

Cited by 31 publications

References 31 publications

Modulating Alginate Hydrogels for Improved Biological Performance as Cellular 3D Microenvironments

Modulating Alginate Hydrogels for Improved Biological Performance as Cellular 3D Microenvironments

Current Perspective and Advancements of Alginate-Based Transplantation Technologies

Polymeric Approaches to Reduce Tissue Responses Against Devices Applied for Islet-Cell Encapsulation

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