Optimization of algae production on urine

Tuantet, Kanjana; Temmink, Hardy; Zeeman, G.; Wijffels, René H.; Buisman, C.J.N.; Janssen, Marcel

doi:10.1016/j.algal.2019.101667

Cited by 24 publications

(19 citation statements)

References 46 publications

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“…The diluted human urine (30 times) was pumped from the feed tank (1) into the MPBR by an automatic feed pump (2). The permeate was intermittently withdrawn in a cycle (8 min of operation and 2 min idle) by a suction pump.…”

Section: Operating Conditions Of the Mpbr Systemmentioning

confidence: 99%

“…Additionally, it offers a high potential to cultivate microalgae for nutrient recovery. Microalgae biomass production is a potential source of feedstock for the bio-based production of biochemicals, biofuels, fertilizer, feed for cattle, food for health, and cosmetics for humans [2]. In addition, many types of wastewaters from agricultural, industrial, synthetic, and municipal activities which have been used for microalgae cultivation coupling with wastewater treatment is regarded as a more economical and sustainable option [3,4].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…There was minimal available knowledge regarding microalgae cultivation by using human urine as a substrate incorporated with a membrane photobioreactor [2]. In several previous studies, synthetic or real urine was applied as a nutrient medium for microalgae growth [2,8,9]. However, ammonia production, high pH, and keyelement precipitation that occurred during urea hydrolysis in concentrated urine would produce microalgae growth difficulties and render nutrient recovery ineffective [9].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Influence of microalgae retention time on biomass production in membrane photobioreactor using human urine as substrate

Thuan¹,

Thuy²,

Hai³

et al. 2018

VJSTE

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Human urine is known as the excreta with a high concentration of nitrogen and phosphorus, causing eutrophication in water bodies. In this study, human urine was used to feed microalgae (Chlorella vulgaris) in a membrane photobioreactor (MPBR) at various microalgae retention times (MRTs) and hydraulic retention time (HRT) of 2 days to evaluate its biomass production. The results indicate that MPBR was operated under MRT of 2 to 5 days and HRT of 2 days, which performed the optimum condition with biomass productivity from 146.43±8.52 to 151.93±15.05 mg.l-1 .day. Moreover, the MPBR using the urine as a nutrient source demonstrated the high performance in biomass production and strong growth of microalgae.

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Section: Operating Conditions Of the Mpbr Systemmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Influence of microalgae retention time on biomass production in membrane photobioreactor using human urine as substrate

Thuan¹,

Thuy²,

Hai³

et al. 2018

VJSTE

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show abstract

“…Bioregenerative ECLS technologies, specifically algal photobioreactors, can simultaneously fix atmospheric carbon dioxide and produce oxygen through photosynthesis. Additional species-dependent functionalities include waste remediation (through nitrogen and phosphorous assimilation) and producing edible biomass (thereby providing carbon and nutrient loop closure) ( Chinnasamy et al, 2009 ; Kumar et al, 2010 ; Jaatinen et al, 2015 ; Tuantet et al, 2019 ). Preliminary studies using photobioreactors for the support of human spaceflight were conducted both terrestrially and in LEO ( Popova et al, 1989 ; Gilles et al, 2008 ; Lasseur et al, 2011 ; Helisch et al, 2020 ; Poughon et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Supporting Simultaneous Air Revitalization and Thermal Control in a Crewed Habitat With Temperate Chlorella vulgaris and Eurythermic Antarctic Chlorophyta

2021

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Including a multifunctional, bioregenerative algal photobioreactor for simultaneous air revitalization and thermal control may aid in carbon loop closure for long-duration surface habitats. However, using water-based algal media as a cabin heat sink may expose the contained culture to a dynamic, low temperature environment. Including psychrotolerant microalgae, native to these temperature regimes, in the photobioreactor may contribute to system stability. This paper assesses the impact of a cycled temperature environment, reflective of spacecraft thermal loops, to the oxygen provision capability of temperate Chlorella vulgaris and eurythermic Antarctic Chlorophyta. The tested 28-min temperature cycles reflected the internal thermal control loops of the International Space Station (C. vulgaris, 9–27°C; Chlorophyta-Ant, 4–14°C) and included a constant temperature control (10°C). Both sample types of the cycled temperature condition concluded with increased oxygen production rates (C. vulgaris; initial: 0.013 mgO2 L–1, final: 3.15 mgO2 L–1 and Chlorophyta-Ant; initial: 0.653 mgO2 L–1, final: 1.03 mgO2 L–1) and culture growth, suggesting environmental acclimation. Antarctic sample conditions exhibited increases or sustainment of oxygen production rates normalized by biomass dry weight, while both C. vulgaris sample conditions decreased oxygen production per biomass. However, even with the temperature-induced reduction, cycled temperature C. vulgaris had a significantly higher normalized oxygen production rate than Antarctic Chlorophyta. Chlorophyll fluorometry measurements showed that the cycled temperature conditions did not overly stress both sample types (FV/FM: 0.6–0.75), but the Antarctic Chlorophyta sample had significantly higher fluorometry readings than its C. vulgaris counterpart (F = 6.26, P < 0.05). The steady state C. vulgaris condition had significantly lower fluorometry readings than all other conditions (FV/FM: 0.34), suggesting a stressed culture. This study compares the results to similar experiments conducted in steady state or diurnally cycled temperature conditions. Recommendations for surface system implementation are based off the presented results. The preliminary findings imply that both C. vulgaris and Antarctic Chlorophyta can withstand the dynamic temperature environment reflective of a thermal control loop and these data can be used for future design models.

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Growth parameter estimation and model simulation for three industrially relevant microalgae: Picochlorum, Nannochloropsis, and Neochloris

Barten

Chin-On

Vree

et al. 2022

Biotech & Bioengineering

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Multiple models have been developed in the field to simulate growth and product accumulation of microalgal cultures. These models heavily depend on the accurate estimation of growth parameters. In this paper growth parameters are presented for three industrially relevant microalgae species: Nannochloropsis sp., Neochloris oleoabundans, and Picochlorum sp. (BPE23). Dedicated growth experiments were done in photobioreactors to determine the maximal biomass yield on light and maintenance rate, while oxygen evolution experiments were performed to estimate the maximal specific growth rate. Picochlorum sp. exhibited the highest specific growth rate of 4.98 ± 0.24 day −1 and the lowest specific maintenance rate of 0.079 day −1 , whereas N. oleoabundans showed the highest biomass yield on light of 1.78 g x •mol ph −1 . The measured growth parameters were used in a simple kinetic growth model for verification. When simulating growth under light conditions as found at Bonaire (12 °N, 68°W), Picochlorum sp. displayed the highest areal biomass productivity of 32.2 g.m −2 •day −1 and photosynthetic efficiency of 2.8%. The

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Optimization of algae production on urine

Cited by 24 publications

References 46 publications

Influence of microalgae retention time on biomass production in membrane photobioreactor using human urine as substrate

Influence of microalgae retention time on biomass production in membrane photobioreactor using human urine as substrate

Supporting Simultaneous Air Revitalization and Thermal Control in a Crewed Habitat With Temperate Chlorella vulgaris and Eurythermic Antarctic Chlorophyta

Growth parameter estimation and model simulation for three industrially relevant microalgae: Picochlorum, Nannochloropsis, and Neochloris

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