Optimization of Agaricus blazei laccase production by submerged cultivation with sugarcane molasses

Valle, Juliana Silveira do; Vandenberghe, Luciana Porto de Souza; Santana, Thiago Teodoro; Linde, Giani Andréa; Colauto, Nelson Barros; Soccol, Carlos Ricardo

doi:10.5897/ajmr2013.6508

Cited by 21 publications

(16 citation statements)

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“…The production of laccase at peak activity and after culture condition optimization using sugarcane molasses was 8.5-fold higher than that reported by Zimbardi et al (2016) for the RP15 strain of P. sanguineus cultivated in wheat bran (5900 U/L) under solid state fermentation. The production of laccase by P. sanguineus U13-4 was 5-fold higher than laccase production by Agaricus blazei (9700 U/L), also cultivated with sugarcane molasses (Valle et al, 2014a). Sugarcane molasses is rich in sugars (mostly sucrose and glucose), and the easily assimilated substrates present in this by-product seem to favor laccase production.…”

Section: Discussionmentioning

confidence: 85%

“…It is rich in fermentable sugars as well as amino acids, vitamins, proteins, and minerals. Owing to its composition and large-scale production, sugarcane molasses can be used as a supplement in animal feed and in ethanol production, and it is a potential substrate for biotechnological processes such as enzyme production (Valle et al, 2014a). The aim of this study was to cultivate P. sanguineus in sugarcane molasses with two different nitrogen sources and various concentrations of copper and nitrogen, with and without agitation, to evaluate laccase production and its dye decolorization capability.…”

Section: Introductionmentioning

confidence: 99%

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Use of sugarcane molasses by Pycnoporus sanguineus for the production of laccase for dye decolorization

Marim¹,

Oliveira²,

Marquezoni³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Pycnoporus sanguineus is a white-rot basidiomycete that produces laccase as the only oxidoreductase; enzyme synthesis depends on cultivation variables, and fungal species and strain. Laccases have wide substrate specificity, oxidize a broad range of compounds, and show potential for use in dye decolorization. We evaluated laccase production in a recently isolated strain of P. sanguineus cultivated with sugarcane molasses as the only carbon source, and urea or yeast urea) increased enzymatic activity. The addition of up to 300 µM of Cu did not affect laccase production, whereas cultivation with agitation increased the activity peak by 17%. The highest laccase activity was ~50,000 U/L on the ninth day of cultivation. After 24 h, decolorization was 80% for Remazol Brilliant Blue R, 9% for Reactive Yellow 145, 6% for Reactive Red 195, and 2% for Reactive Black 5. The enzymatic extract of P. sanguineus provides a potential alternative to wastewater treatment. A better understanding of the behavior of this fungus under various culture conditions would allow improvement of the enzyme production bioprocess.

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Section: Discussionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Use of sugarcane molasses by Pycnoporus sanguineus for the production of laccase for dye decolorization

Marim¹,

Oliveira²,

Marquezoni³

et al. 2016

Genet. Mol. Res.

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“…O cobre é conhecido como um indutor da atividade de lacase para muitas espécies de fungos (VALLE et al, 2014) e parece influenciar linhagens de P. sanguineus de forma distinta. A adição de 150 µM de cobre aumentou a atividade de lacase de forma significativa (p≤0,05) (~34300 U/L), resultados similares aos descritos por Fonseca et al (2010) que relataram aumento na atividade de lacase de P. sanguineus BAFC 2126 na presença de 500 µM de cobre.…”

Section: Discussionunclassified

“…A produção de lacase foi conduzida em frascos Erlenmeyer (250 mL) contendo 100 mL de meio de cultivo composto por ureia (2,4 g/L de nitrogênio), fosfato de potás-sio monobásico (1 g/L); sulfato de magnésio heptahidratado (0,5 g/L); sulfato ferroso heptahidratado (0,01 g/L) (VALLE et al, 2014) suplementado com melaço de soja (MS) como única fonte de carbono. O MS foi diluído em água destilada e incorporado ao meio em quantidade suficiente para obter--se concentração final de açúcares totais de 10 g/L.…”

Section: Produção De Lacaseunclassified

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PRODUÇÃO DE LACASE DE Pycnoporus sanguineus EM MEIO DE CULTIVO À BASE DE MELAÇO SOJA

Fabrini

Avelino

Marim

et al. 2017

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Umuarama, v. 19, n. 3, p. 159-164, jul./set. 2016. RESUMO:Lacases são polifenol oxidases que utilizam a capacidade redox de íons cobre para reduzir oxigênio a água e oxidar um substrato fenólico. A síntese e secreção de lacases de basidiomicetos dependem de vários fatores como os nutrientes presentes no meio de cultura. Visando à produção de lacase, Pycnoporus sanguineus foi cultivado em meio contendo melaço de soja como única fonte de carbono, ureia como fonte de nitrogênio suplementar em diferentes concentrações (0,6; 1,2; 2,4; 4,8 e 9,6 g/L de nitrogênio) e diferentes concentrações de CuSO 4 (0, 150, 200, 250 e 300 µM). O extrato enzimático produzido nas melhores condições de cultivo foi utilizado para a descoloração dos corantes remazol azul brilhante R (antraquinona), amarelo 145, preto 5, vermelho 195 (azo) e verde malaquita (trifenilmetano). As concentrações de nitrogênio não afetaram a produção de lacase, exceto a maior concentração (9,6 g/L) que reduziu a atividade enzimática. A adição de cobre ao meio de cultivo (150 µM) aumentou a atividade de lacase em 112%. A maior atividade de lacase (~34300 U/L) promoveu a descoloração dos corantes remazol azul brilhante R (67,5%) e verde malaquita (28,3%) em 24h, sendo os corantes azo descoloridos apenas parcialmente. Concluiu-se que o melaço de soja é um resíduo agroindustrial adequado para produção de lacase de P. sanguineus com potencial para degradação de corantes. PALAVRAS-CHAVE: Antraquinona. Corante azo. Ligninases. Poliporaceae. Resíduos agroindustriais. LACCASE PRODUCTION BY Pycnoporus sanguineus IN CULTURE MEDIA WITH SOYBEAN MOLASSESABSTRACT: Laccases are multicopper oxidases using the redox ability from copper ions to reduce oxygen to water, while oxidizing a phenolic substrate. Laccase synthesis and secretion in basidiomycetes depend on the conditions provided and on the nutrients present in the culture medium. Pycnoporus sanguineus was cultivated in medium containing soybean molasses as the sole carbon source, with urea as the source of supplemental nitrogen at different concentrations (0.6, 1.2, 2.4, 4.8 and 9.6 g/L nitrogen), and different CuSO 4 concentrations (0, 150, 200, 250 and 300 μM). The enzymatic extract produced under the best cultivation conditions was used for the depigmentation of remazole brilliant blue R (anthraquinone), yellow 145, black 5, red 195 (azo) and malachite green (triphenylmethane). Nitrogen concentrations did not affect laccase production, except for the higher concentration (9.6 g/L) reducing enzymatic activity. The addition of copper to the culture medium (150 μM) increased laccase activity by 112%. The highest laccase activity (~34300 U/L) promoted the depigmentation of remazol brilliant blue R (67.5%) and malachite green (28.3%) dyes in 24 hours. Azo dyes were only partially discolored. Therefore, it can be considered that soybean molasses is an agro-industrial byproduct suitable for the production of P. sanguineus laccase with potential for dye degradation. KEYWORDS: Agro-industrial byproduct. Anthraquinone. Azo d...

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Delignification and detoxification of peanut shell bio-waste using an extremely halophilic laccase from an Aquisalibacillus elongatus isolate

et al. 2017

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Lignocellulose bioconversion is a harsh process requiring the use of surfactants and organic solvents. Consequently, the incorporation of laccases in this bioconversion requires the bioprospecting of enzymes that can remain stable under extreme conditions. An extracellular, highly stable laccase was produced by the halophilic isolate Aquisalibacillus elongatus in submerged liquid culture fermentation. Statistical and non-statistical strategies gave the highest enzymatic activity (8.02 U mL) following addition of glucose (1.7 g L), copper sulfate (0.8 g L), urea (15 g L), and CaCl (0.8 g L). The enzyme, after purification using a synthetic affinity support, delignified a peanut shell substrate by 45%. A pH of 8.0 and a temperature of 35 °C were optimal for delignification of this bio-waste material. Addition of [Bmim][PF], 1,4-dioxane, acetone, and HBT promoted this bio-waste delignification. Bio-treatment in the presence of 50% [Bmim][PF] gave a maximal lignin removal of 87%. The surfactants tested had no significant effects on the delignification yield. The laccase also detoxified the toxic phenols found in peanut shell waste. The high catalytic efficiency of this enzyme against a lignocellulosic sample under extreme conditions suggests the suitability of this laccase for industrial applications.

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Optimization of Agaricus blazei laccase production by submerged cultivation with sugarcane molasses

Cited by 21 publications

References 42 publications

Use of sugarcane molasses by Pycnoporus sanguineus for the production of laccase for dye decolorization

Use of sugarcane molasses by Pycnoporus sanguineus for the production of laccase for dye decolorization

PRODUÇÃO DE LACASE DE Pycnoporus sanguineus EM MEIO DE CULTIVO À BASE DE MELAÇO SOJA

Delignification and detoxification of peanut shell bio-waste using an extremely halophilic laccase from an Aquisalibacillus elongatus isolate

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