Optimization of Activated Charcoal on in vitro Growth and Development of Potato (Solanum tuberosum L.)

Buckseth, Tanuja; Singh, Rakesh Kumar; Sharma, Ashwani; Sharma, Sumita; Moudgil, Vaishali; Saraswati, Aastha

doi:10.20546/ijcmas.2018.710.410

Cited by 7 publications

(2 citation statements)

References 15 publications

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“…The findings were consistent with the previous results obtained in other plants, such as potato (Buckseth et al, 2018), sugarcane (Shimelis et al, 2015), carrots (Pan and Staden, 2001), and grapevine (Olah, 2017). The arbitrary visual hedonic scales used in this study could be used in future status to assess callus browning intensity.…”

Section: Discussionsupporting

confidence: 93%

Dragon fruit calli development and fungal contamination as influenced by activated charcoal

Cruz

Balendres

2021

J PROF ASSOC CACTUS

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Activated charcoal is often used in plant tissue culture systems to reduce phenolic oxidation and improve cell growth and development. This study investigated activated charcoal's effect on the somatic embryogenesis of dragon fruit (Hylocereus species). Nine-month-old seed-derived dragon fruit stems wereplaced in basal Murashige and Skoog (MS) plates supplemented with 2, 4-D (1.5 mg/l) and activated charcoal (1g/l). Cultures were incubated in 14 h continuous light or under dark condition. MS medium without activated charcoal served as the control. Percent fungal contamination, degree of tissue browning, and callus growth (length, width, and total area) were assessed. A significant decrease in contamination was observed four days after inoculation (dai) in a medium containing activated charcoal regardless of the light condition used (p<0.05, T-test analysis). In contrast, no significant difference in fungal contamination between the two treatments was found eight days after incubation (dai). Fungal contaminants’ identities were done using a combined morphocultural and molecular analyses. The fungal isolates showed thick and white to grayish mycelia without spore production seven dai in potato dextrose agar medium. DNA sequence analysis showed a high percent similarity of the isolates to two Diaporthe species. Activated charcoal did not affect the length, width, and total area of the calli produced. However, a significant decrease in tissue browning was recorded (p<0.05). Overall results demonstrated the applicability of activated charcoal in dragon fruit tissue culture to reduce in-vitrobrowning. Additionally, examining further the possible role of fungal contaminants in dragon fruits would be valuable. A hedonic scale that can be used to assess tissue browning in dragon fruit is also described.

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Section: Discussionsupporting

confidence: 93%

Dragon fruit calli development and fungal contamination as influenced by activated charcoal

Cruz

Balendres

2021

J PROF ASSOC CACTUS

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“…The addition of activated charcoal (AC) in Activated charcoal is frequently added to plant culture media to promote cell growth and development. It provides a dark environment that can stimulate nitrogen uptake by shoots and promote rooting in vitro [21]. Activated charcoal can reduce toxic effect due to its capability in the adsorption of toxic compounds in culture media.…”

Section: Root Induction and Plantlet Regenerationmentioning

confidence: 99%

The effect of colchicine on in vitro growth of ciplukan (Physalis angulata)

Mastuti

Munawarti

Afiyanti

2022

IOP Conf. Ser.: Earth Environ. Sci.

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Ciplukan (Physalis angulata) is one of medicinal plants belongs to Solanaceae family. The in vitro system has the potential to multiply plants that have uniform characters in large quantities. One strategy to improve the important characters of medicinal plants is by artificially induced polyploidy using antimitotic compounds, such as colchicine. Variations in types of explants treated with colchicine have varied characters in growth response. Therefore, the objective of this study was to investigate the effect of tissue types and different duration of soaking in colchicine solution on the shoot and root regeneration of P. angulata in vitro. Two types of tissue namely seeds, and cotyledonary nodes were soaked in 0.1% colchicine solution for 0, 24, 48 and 72 hours. Shoot regeneration of control and colchicine-treated tissues were cultured on MS medium supplemented with BAP 2 mg/l + IAA 0.05 mg/L. Rooting medium was MS0 medium with or without supplemented with activated charcoal (AC). The results showed that the number and height of shoots and the number of leaves on the growth of shoots in vitro had the same pattern except for in vitro shoots produced by seeds soaked in colchicine solution for 48 hours. In vitro shoots produced by soaking cotyledonary nodes for 24 hours tended to produce better growth than shoots produced from seeds without colchicine treatment (control) as well as 48- and 72-hours soaking treatments. The in vitro shoots produced by soaking the seeds for 72 hours still tended to be able to produce better growth than the control and soaking treatments for 24- and 48-hours. Shoot derived from colchicine treated cotyledonary nodes produced more roots than shoots produced by soaking seeds. However, shoot height and number of leaves varied depending on the duration of soaking of explants in colchicine solution. Activated charcoal reduced root number.

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Novel Rapid In Vitro Method for Screening of Potato Genotypes Against Streptomyces sp.

Maharana,

Sagar,

Sharma

et al. 2024

Potato Res.

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Optimization of Activated Charcoal on in vitro Growth and Development of Potato (Solanum tuberosum L.)

Cited by 7 publications

References 15 publications

Dragon fruit calli development and fungal contamination as influenced by activated charcoal

Dragon fruit calli development and fungal contamination as influenced by activated charcoal

The effect of colchicine on in vitro growth of ciplukan (Physalis angulata)

Novel Rapid In Vitro Method for Screening of Potato Genotypes Against Streptomyces sp.

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