Optimization of a spray drying process to prepare dry powder microparticles containing plasmid nanocomplex

Mohajel, Nasir; Najafabadi, Abdolhossein R.; Azadmanesh, Kayhan; Vatanara, Alireza; Moazeni, Esmaeil; Rahimi, Amir Abbas; Gilani, Kambiz

doi:10.1016/j.ijpharm.2011.11.014

Cited by 40 publications

(21 citation statements)

References 47 publications

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“…On the other hand, for high inlet feed rates the droplets might be unable to get enough hot air for a complete evaporation, sticking to the chamber wall. The results are similar to that reported by other researchers [29,30].…”

Section: Resultssupporting

confidence: 93%

Optimization of Spray Drying Process Parameters for Sweet Corn Enzymolysis Liquid

Jiang

Liu

2015

International Journal of Food Engineering

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The aim of this study was to optimize the spray drying process parameters for sweet corn enzymolysis liquid concentrate (SCELC). Experimental design with inlet temperature (170-190°C), SCELC/maltodextrin (MD) ratio (0.25-4) and inlet feed rate (800-1000 mL h −1 ) as independent variables was studied to investigate the effects on product responses. The corresponding results showed that an increase in the inlet temperature resulted in sweet corn powder with lower moisture, bulk density, L* value, H°value and higher water solubility index. Meanwhile, an increase in MD ratio brought lower moisture and higher L* value, H°value, water solubility index. It was also found that an increase in the inlet feed rate caused higher moisture and lower water solubility index. The highest production yield was achieved at 42.86% with optimized inlet temperature of 177.66°C, SCELC/MD ratio of 0.84 and inlet feed rate of 834.50 mL h −1 . Accordingly, the production yield, moisture content, bulk density, water solubility index, L* and H°value were 42.86%, 3.57%, 0.45 g/cm 3 , 96.54 g/100 g, 84.46, and 83.54, respectively.

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Section: Resultssupporting

confidence: 93%

Optimization of Spray Drying Process Parameters for Sweet Corn Enzymolysis Liquid

Jiang

Liu

2015

International Journal of Food Engineering

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“…It was noticed that as the inlet temperature increased from 50°C to 100°C there was an increase in powder yield%. It has been reported that a high inlet temperature can reduce the drying time and inhibit particle aggregation . Furthermore, a higher inlet temperature promotes a decrease in residual moisture by enhancing water evaporation resulting in less particles sticking in the drying chamber …”

Section: Discussionmentioning

confidence: 99%

Pulmonary Delivery of Proteins Using Nanocomposite Microcarriers

Alfagih

Kunda

Alanazi

et al. 2015

Journal of Pharmaceutical Sciences

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Pneumonia, caused by Streptococcus pneumoniae, mainly affects the immunocompromised, the very young and the old, and remains one of the leading causes of death. A steady rise in disease numbers from non-vaccine serotypes necessitates a new vaccine formulation that ideally has better antigen stability and integrity, does not require cold-chain and can be delivered non-invasively. In this study, a dry powder vaccine containing an important antigen of S. pneumoniae, pneumococcal surface protein A (PspA) that has shown cross-reactivity amongst serotypes to be delivered via the pulmonary route has been formulated. The formulation contains the antigen PspA adsorbed onto the surface of polymeric nanoparticles encapsulated in L-leucine microparticles that can be loaded into capsules and delivered via an inhaler. We have successfully synthesized particles of ∼150 nm and achieved ∼20 μg of PspA adsorption per mg of NPs. In addition, the spray-dried powders displayed a FPF of 74.31 ± 1.32% and MMAD of 1.70 ± 0.03 μm suggesting a broncho-alveolar lung deposition facilitating the uptake of the nanoparticles by dendritic cells. Also, the PspA released from the dry powders maintained antigen stability (SDS-PAGE), integrity (Circular dichroism) and activity (lactoferrin binding assay). Moreover, the released antigen also maintained its antigenicity as determined by ELISA. Graphical abstractPlease cite this article as: Kunda, Nitesh K., Alfagih, Iman M., Miyaji, Eliane N.

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“…It has been reported that the spraydrying condition that gives particles with low moisture content may also give a better yield. 29) In Fig. 2, it can be seen that, irrespective of inlet temperature, yield significantly increased as the feed rate was decreased.…”

Section: Box-behnken Statistical Analysis and Model Fitting Of The Datamentioning

confidence: 92%

Statistical modeling, optimization and characterization of spray-dried solid self-microemulsifying drug delivery system using design of experiments

Marasini

Tran

Poudel

et al. 2013

CHEMICAL & PHARMACEUTICAL BULLETIN

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The present study systematically and simultaneously investigates the influence of process variables of spray-drying on the properties of solid self-microemulsifying drug delivery system (SmeddS) using design of experiment (DOE) and optimizes them in order to produce solid-SMEDDS satisfying pre-defined powder quality attributes. Flurbiprofen-loaded liquid-SMEDDS was dispersed in dextran and spray-dried. After preliminary screening, the independent factors selected according to three-factor, three-level Box-Behnken design were inlet temperature (X 1 ), feed rate (X 2 ) and carrier concentration (X 3 ). The responses used to compute the effects of independent factors were moisture content (Y 1 ), yield (Y 2 ), drug content (Y 3 ) and droplet size (Y 4 ) of the micro-emulsion. SMEDDS powder characteristics such as morphology, thermal behavior, crystallinity and flowability were also considered. Models were developed and model fitting analysis showed an adequate fit for all responses, indicating good predictability. Significant effects of processing parameters on powder characteristics were observed. The spray-drying process parameters were optimized as inlet temperature (134°C), feed rate (5%) and carrier concentration (0.6%) to produce solid-SMEDDS with acceptable moisture content (0.72±0.02%), yield (58.5±2.9%), drug content (70.1±2.7 mg/g) and droplet size (166.8±13.8 nm). Validation of the optimization process in five batches showed experimental value very close to the predicted one, ensuring the reproducibility of the developed models. Furthermore, optimized parameters resulted a highly crystalline flurbiprofen changed to an amorphous form. In conclusion, this study demonstrated the applicability of the DOE approach for optimizing the process parameters to manufacture solid-SMEDDS with desired quality attributes.Key words design of experiment; Box-Behnken design; optimization; self-microemulsifying drug delivery system; spray-drying Self-microemulsifying drug delivery system (SMEDDS) has been emphasized in recent years for its ability to improve the solubility and bioavailability of poorly water-soluble drugs. Conventionally, SMEDDS are prepared as liquid formulations that are either delivered in soft or hard gelatin capsules 1) However, a liquid form of SMEDDS has several potential drawbacks in manufacturing scale-up such as less stability due to the precipitation of active ingredients, especially at lower temperatures, interactions of formulation components with the capsule shells, and leakage from the capsules.2,3) In order to overcome these limitations, the liquid-based dispersion must be converted into a dry product. In addition, a solid form of SMEDDS formulation combines the advantages of enhanced solubility and bioavailability with a higher stability compared to its corresponding liquid formulation. 4) Solidification can be achieved by dispersion of liquid SMEDDS into a suitable inert solid carrier in an aqueous medium, followed by elimination of the water via several techniques such as spray-drying. Th...

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Optimization of a spray drying process to prepare dry powder microparticles containing plasmid nanocomplex

Cited by 40 publications

References 47 publications

Optimization of Spray Drying Process Parameters for Sweet Corn Enzymolysis Liquid

Optimization of Spray Drying Process Parameters for Sweet Corn Enzymolysis Liquid

Pulmonary Delivery of Proteins Using Nanocomposite Microcarriers

Statistical modeling, optimization and characterization of spray-dried solid self-microemulsifying drug delivery system using design of experiments

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