Optimization of a simple, accurate and low cost method for starch quantification in green microalgae

Yong, Tze Ching; Chiu, Chia-Sheng; Chen, Ching-Nen Nathan

doi:10.1186/s40529-019-0273-y

Cited by 3 publications

(1 citation statement)

References 16 publications

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“…The biomass harvested from wastewater treatment can also be utilized as raw materials for biofuel, feed, and chemical compounds [4][5][6][7][8][9]. Microalgae can use CO 2 during photosynthesis as the only carbon source for heterotrophic growth and can use external carbon sources for heterotrophic growth to improve the biomass and oil content of microalgae [10,11], which can greatly reduce global emissions of greenhouse gases such as CO 2 [12].…”

Section: Introductionmentioning

confidence: 99%

A new Desmodesmus sp. from the Tibetan Yamdrok Lake

et al. 2022

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Revegetation of exposed sub-soil, while a desirable recovery strategy, often fails due to extreme soil chemical properties, such as low organic matter and pH levels. Microalgae play a key role in maintaining water quality in the lakes and rivers on the Qinghai-Tibet plateau. Plateau microalgae have extensive application prospects in environmental purification, biotechnology, medicine and cosmetics, food industry, and renewable energy. To identify the high biomass of microalgae present in nature, microalgae with the greatest biomass were screened from natural water samples through filtration, pre-culture, and plate scribing separation. Following identification via 18S rRNA sequencing as for the Desmodesmus sp., we constructed a neighbor-joining phylogenetic tree. The novel Desmodesmus sp. from the Tibetan Yamdrok Lake were identified through polyphasic taxonomy. Simultaneously, the sequence of the experimental samples and the target species were shown different following the identification and analysis of SNP and InDel loci. The light-absorbing properties of plateau Desmodesmus sp. have been investigated previously. The characteristic absorption peak of Desmodesmus sp. on the plateau was measured at 689 nm in the visible spectrum using full wavelength scanning with a UV-Vis spectrophotometer. For Desmodesmus sp. which is prone to settling in the process of amplification culture. By monitoring the change trend of total nitrogen, total phosphorus, pH and electrical conductivity in algae solution system, we determined that the logarithmic growth phase and the best transfer window of Desmodesmus sp. were at 15–20 days. This study can provide basic research methods for the study of microalgae in high altitude areas, and lay a foundation for the later study and application of microalgae.

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Section: Introductionmentioning

confidence: 99%

A new Desmodesmus sp. from the Tibetan Yamdrok Lake

et al. 2022

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Algae-bacterial aquaculture can enhance heavy metals (Pb2+ and Cd2+) remediation and water re-use efficiency of synthetic streams

Chandrashekharaiah

Gupte

Sarkar

et al. 2022

Resources, Conservation and Recycling

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Optimized Spectrophotometry Method for Starch Quantification

et al. 2022

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Starch is a polysaccharide that is abundantly found in nature and is generally used as an energy source and energy storage in many biological and environmental processes. Naturally, starch tends to be in miniscule amounts, creating a necessity for quantitative analysis of starch in low-concentration samples. Existing studies that are based on the spectrophotometric detection of starch using the colorful amylose–iodine complex lack a detailed description of the analytical procedure and important parameters. In the present study, this spectrophotometry method was optimized, tested, and applied to studying starch content of atmospheric bioaerosols such as pollen, fungi, bacteria, and algae, whose chemical composition is not well known. Different experimental parameters, including pH, iodine solution concentrations, and starch solution stability, were tested, and method detection limit (MDL) and limit of quantification (LOQ) were determined at 590 nm. It was found that the highest spectrophotometry signal for the same starch concentration occurs at pH 6.0, with an iodine reagent concentration of 0.2%. The MDL was determined to be 0.22 μg/mL, with an LOQ of 0.79 μg/mL. This optimized method was successfully tested on bioaerosols and can be used to determine starch content in low-concentration samples. Starch content in bioaerosols ranged from 0.45 ± 0.05 (in bacteria) to 4.3 ± 0.06 μg/mg (in fungi).

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Optimization of a simple, accurate and low cost method for starch quantification in green microalgae

Cited by 3 publications

References 16 publications

A new Desmodesmus sp. from the Tibetan Yamdrok Lake

A new Desmodesmus sp. from the Tibetan Yamdrok Lake

Algae-bacterial aquaculture can enhance heavy metals (Pb2+ and Cd2+) remediation and water re-use efficiency of synthetic streams

Optimized Spectrophotometry Method for Starch Quantification

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