Optimization of a novel nylon mesh container for human embryo ultrarapid vitrification

Nakashima, Akira; Ino, Nao; Kusumi, Maki; Ohgi, Shirei; Ito, Megumu; Horikawa, Takashi; Nakagawa, Koji; Saito, Toki; Kamura, Toshiharu; Saito, Hidekazu

doi:10.1016/j.fertnstert.2009.01.063

Cited by 24 publications

(22 citation statements)

References 16 publications

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“…This method was shown to be rapid, inexpensive and effective method of embryo cryopreservation. However, there is a chance of few embryos being lost when immersed in the thawing solution [16]. Though the biochemical pregnancy rate shown by beta hCG values more than 50IU/ml was 65%, the acceptable clinical pregnancy rate of 48% in our study reinforces the fact that vitrification is a simple, rapid and effective method of embryo cryopreservation in an ART programme.…”

Section: Discussion:-supporting

confidence: 52%

Embryo Vitrification on Day2 at 4-Cell Stage Using Cryoloop Device: Our Experience.

Jamwal¹,

Mohanlal²,

Sandeep³

et al. 2016

IJAR

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Section: Discussion:-supporting

confidence: 52%

Embryo Vitrification on Day2 at 4-Cell Stage Using Cryoloop Device: Our Experience.

Jamwal¹,

Mohanlal²,

Sandeep³

et al. 2016

IJAR

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“…The toxic effects of the cryoprotectant may be prevented by reducing the equilibration time and lowering the equilibration temperature as well as by reducing the vitrification time and lowering the temperature in vitrified embryos [13]. A reduction in the volume of the cryoprotectant using a cryoloop, electron microscope grid and nylon mesh sheet results in both greater cooling rates and minimization of cryoinjury in vitrified embryos [14,15,16,17,18,19]. …”

Section: Introductionmentioning

confidence: 99%

“…The thawing or warming process is also a major factor in embryo damage [11,17,18,20]. Because frozen or vitrified embryos contain cryoprotectants in each blastomere, water will flow into blastomeres more rapidly as compared to the outward diffusion of the cryoprotectant.…”

Section: Introductionmentioning

confidence: 99%

“…If cells swell and expand beyond a critical volume, the cell membrane will be irreversibly damaged [21]. Although osmotic swelling of embryos during the thawing or warming process is critical for embryo survival, few studies have investigated methods to prevent the adverse effects of osmotic swelling [17,22]. …”

Section: Introductionmentioning

confidence: 99%

“…In contrast to slow freezing, vitrification solutions contain a high concentration of permeable cryoprotectants such as dimethyl sulfoxide and ethylene glycol, which can be as high as 2.1-7.2 M [12,23,26]. Even with such a high concentration of permeable cryoprotectants, sucrose or trehalose concentrations in the warming medium used in vitrification ranged from 0.3 to 1.0 M in animal and human embryos [4,9,13,17,18,27,28,29]. …”

Section: Introductionmentioning

confidence: 99%

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Effects of Different Trehalose Concentrations in a Warming Medium on Embryo Survival and Clinical Outcomes in Vitrified Human Embryos

Matsuo

Takahashi²,

Igarashi³

et al. 2013

Gynecol Obstet Invest

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Background/Aims: In the present study, we examined the effects of different concentrations of trehalose in a warming medium on both embryo survival and clinical outcomes in vitrified-warmed embryo transfer cycles. Methods: We retrospectively analyzed a total of 209 vitrified-warmed cycles from 177 patients who underwent in vitro fertilization or intracytoplasmic sperm injection and embryo transfer. Embryos were cryopreserved by the vitrification method and warmed in solutions containing either 0.5 or 1.0 M trehalose. We compared the 0.5 and 1.0 M trehalose warming solution groups with respect to the embryo survival rate after warming and clinical outcomes. Results: The embryo survival rate in the 1.0 M trehalose group (96.5%) was significantly higher than that in the 0.5 M trehalose group (57.0%). The percentage of embryo transfers after warming in the 1.0 M trehalose group (94.3%) was significantly higher than that in the 0.5 M trehalose group (83.7%). The clinical pregnancy rate in the 1.0 M trehalose group (25.0%) was significantly higher than that in the 0.5 M trehalose group (11.1%). Conclusion: Embryo survival and clinical pregnancy rates were higher when a 1.0 M trehalose solution was used than when a 0.5 M trehalose solution was used during the embryo warming process.

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Conduction‐Dominated Cryomesh for Organism Vitrification

Guo,

Zuchowicz,

Bouwmeester

et al. 2023

Advanced Science

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Vitrification‐based cryopreservation is a promising approach to achieving long‐term storage of biological systems for maintaining biodiversity, healthcare, and sustainable food production. Using the “cryomesh” system achieves rapid cooling and rewarming of biomaterials, but further improvement in cooling rates is needed to increase biosystem viability and the ability to cryopreserve new biosystems. Improved cooling rates and viability are possible by enabling conductive cooling through cryomesh. Conduction‐dominated cryomesh improves cooling rates from twofold to tenfold (i.e., 0.24 to 1.2 × 105 °C min−1) in a variety of biosystems. Higher thermal conductivity, smaller mesh wire diameter and pore size, and minimizing the nitrogen vapor barrier (e.g., vertical plunging in liquid nitrogen) are key parameters to achieving improved vitrification. Conduction‐dominated cryomesh successfully vitrifies coral larvae, Drosophila embryos, and zebrafish embryos with improved outcomes. Not only a theoretical foundation for improved vitrification in µm to mm biosystems but also the capability to scale up for biorepositories and/or agricultural, aquaculture, or scientific use are demonstrated.

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Optimization of a novel nylon mesh container for human embryo ultrarapid vitrification

Cited by 24 publications

References 16 publications

Embryo Vitrification on Day2 at 4-Cell Stage Using Cryoloop Device: Our Experience.

Embryo Vitrification on Day2 at 4-Cell Stage Using Cryoloop Device: Our Experience.

Effects of Different Trehalose Concentrations in a Warming Medium on Embryo Survival and Clinical Outcomes in Vitrified Human Embryos

Conduction‐Dominated Cryomesh for Organism Vitrification

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