Optimization of a New Non-viral Vector for Transfection: Eudragit Nanoparticles for the Delivery of a DNA Plasmid

Abstract

The development of new vectors to deliver DNA into cells for therapy of cancers or genetic diseases has been a major area of research for many years. However, the clinical application of this technology requires the development of efficient, reliable and sterile vectors enabling the transfer of genes in vivo. Non viral, polymer or lipid-based vectors offer a new impetus to gene therapy because they are less toxic than viral vectors (no endogenous recombination, fewer immunological reactions, easy production and delivery of large-sized plasmid).The aim of this study is to develop a new tool for DNA delivery composed of methacrylic polymeric (Eudragit ® RS and RL) nanoparticles. These nanoparticles were prepared by two meth- was between 2 and 4%. Nanoparticles prepared by nanoprecipitation were slightly more efficient than nanoparticles prepared from a double emulsion. Particle size was not an important factor for transfection, since no significant difference was observed with size between 50 and 350 nm. We showed that Eudragit ® RS and RL nanoparticles could introduce the transgene into different types of cells, but were generally less effective than the lipofectamine control.

Cationic Nanomicelles for Delivery of Plasmids Encoding Interleukin-4 and Interleukin-10 for Prevention of Autoimmune Diabetes in Mice

“…A large number of cationic polymers which effectively condense the DNA and mask their negative charge have been investigated for gene therapy (30)(31)(32)(33)(34). Though the viral vectors were initially used as gene carriers, the benefits of non viral polymeric delivery systems are now being fully appreciated.…”

Engineered Nanoparticulate Drug Delivery Systems: The Next Frontier for Oral Administration?

“…Interestingly, it has been found that NPs prepared by nanoprecipitation were slightly more efficient than NPs prepared from a double emulsion. Finally, particle size was not an important factor for transfection, since no significant difference was observed with size between 50 and 350 nm (52).…”

“…In another study, the DNA plasmid has been encapsulated in Eudragit® RS or RL NPs as nonviral vectors (52). NPs were prepared by two methods: nanoprecipitation and double emulsion.…”

Untitled

“…Verma et al, prepared acetazolamide-loaded eudragit RL 100 nanoparticle suspension by nano-precipitation method to increase topical ocular bioavailability and to sustain the release of drug for a longer time 45 . 47 .…”