Optimization of A(H1N1)pdm09 vaccine seed viruses: The source of PB1 and HA vRNA as a major determinant for antigen yield

Almeida, Filipe; Trigueiro-Louro, João

doi:10.1016/j.virusres.2022.198795

Cited by 3 publications

(2 citation statements)

References 46 publications

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“…Y360 of PR8 PB2 is responsible for viral enhancement of polymerase activity and viral growth in MDCK cells but not in eggs ( Murakami, et al, 2008 ). PB1 and HA from the same source in the PR8 internal backbone enhanced the replication efficiency of the H1N1 2009pdm vaccine candidate in embryonated eggs and cell culture ( Mostafa, et al, 2016 ; Almeida, et al, 2022 ). Additionally, the PA E31K mutant of the PR8 internal backbone in the avian influenza vaccine exhibited enhanced replication in Vero cells ( Lee, et al, 2017b ).…”

Section: Discussionmentioning

confidence: 99%

An avian-origin internal backbone effectively increases the H5 subtype avian influenza vaccine candidate yield in both chicken embryonated eggs and MDCK cells

Yang,

Zhao,

Huo

et al. 2024

Poultry Science

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Section: Discussionmentioning

confidence: 99%

An avian-origin internal backbone effectively increases the H5 subtype avian influenza vaccine candidate yield in both chicken embryonated eggs and MDCK cells

Yang,

Zhao,

Huo

et al. 2024

Poultry Science

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“…The M vRNA segment almost always originates from PR8 [ 10 , 16 , 17 ] and has been shown to confer a higher yield [ 11 ]. Analysis of 5:3 reassortants has revealed that the PB1 vRNA segment is frequently derived from the circulating virus [ 10 , 16 , 17 ] by conferring a higher yield [ 18 , 19 , 20 , 21 ]. Thus, the high yield of a candidate vaccine virus is often conferred by the PR8 M vRNA segment and the PB1 vRNA segment of the circulating virus.…”

Section: Discussionmentioning

confidence: 99%

Development of an Enhanced High-Yield Influenza Vaccine Backbone in Embryonated Chicken Eggs

et al. 2023

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Vaccination is an efficient approach to preventing influenza virus infections. Recently, we developed influenza A and B virus vaccine backbones that increased the yield of several vaccine viruses in Madin–Darby canine kidney (MDCK) and African green monkey kidney (Vero) cells. These vaccine backbones also increased viral replication in embryonated chicken eggs, which are the most frequently used platform for influenza vaccine manufacturing. In this study, to further increase the viral titers in embryonated chicken eggs, we introduced random mutations into the ‘internal genes’ (i.e., all influenza viral genes except those encoding the hemagglutinin and neuraminidase proteins) of the influenza A virus high-yield virus backbone we developed previously. The randomly mutated viruses were sequentially passaged in embryonated chicken eggs to select variants with increased replicative ability. We identified a candidate that conferred higher influenza virus growth than the high-yield parental virus backbone. Although the observed increases in virus growth may be considered small, they are highly relevant for vaccine manufacturers.

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Adaptive evolution of PB1 from influenza A(H1N1)pdm09 virus towards an enhanced fitness

Almeida¹,

Gíria²,

Trigueiro-Louro³

et al. 2023

Virology

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Optimization of A(H1N1)pdm09 vaccine seed viruses: The source of PB1 and HA vRNA as a major determinant for antigen yield

Cited by 3 publications

References 46 publications

An avian-origin internal backbone effectively increases the H5 subtype avian influenza vaccine candidate yield in both chicken embryonated eggs and MDCK cells

An avian-origin internal backbone effectively increases the H5 subtype avian influenza vaccine candidate yield in both chicken embryonated eggs and MDCK cells

Development of an Enhanced High-Yield Influenza Vaccine Backbone in Embryonated Chicken Eggs

Adaptive evolution of PB1 from influenza A(H1N1)pdm09 virus towards an enhanced fitness

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