Optimization of a foot-and-mouth disease virus Southern African Territories–specific solid-phase competitive ELISA for small ruminant serum samples
LaToya Seoke,
Geoffrey T. Fosgate,
Pamela A. Opperman
et al.
Abstract:We optimized and verified a single-spot solid-phase competitive ELISA (ss-SPCE) to detect antibodies against structural proteins of Southern African Territories (SAT) serotypes of foot-and-mouth disease virus (FMDV) in small ruminants. Sera from goats vaccinated and experimentally challenged with a SAT1 FMDV pool were tested in duplicate at 4 dilutions (1:10, 1:15, 1:22.5, 1:33.8) to optimize the assay. To assess the performance of the assay in naturally infected animals, we evaluated 316 goat and sheep field … Show more
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