Optimization of a Culture Medium Using the Taguchi Approach for the Production of Microorganisms Active in Odorous Compound Removal

Abstract

Abstract:The aim of this work was to develop the composition of a medium for the cultivation of six microbial strains forming a deodorizing consortium: Pseudomonas fluorescens, Enterococcus faecium, Bacillus subtilis, Bacillus megaterium, Leuconostoc mesenteroides and Lactobacillus plantarum. The study focused on the optimization of a highly efficient culture medium composed of readily available components of plant origin to maximize microbial biomass yields, and to create a less expensive alternative to the commercial Tryptic Soy Broth medium (TSB). After preliminary efficiency screening of all tested media components, we selected four substrates for further optimization-soy protein concentrate (SPC), glucose or sucrose, and phosphate salts. The final concentrations of all components were fine-tuned using the Taguchi design for experiments according to an L 9 array. Taguchi optimization led to formulation of a culture medium, which was approximately 5 times cheaper than TSB (depending on the components used). Consequently, microbial biomass yields were improved by up to 15-fold (1564%), depending on the strain. The results obtained in the laboratory experiments were then confirmed in pilot-(42 L) and industrial-(300 L) scale fermentation. Our results show that this method of using a parallel culture microbioreactor with the Taguchi approach can be recommended for optimization of culture media based on substrates of plant origin.

Comparative Studies of Inhibitory and Antioxidant Activities, and Organic Acids Compositions of Postbiotics Produced by Probiotic Lactiplantibacillus plantarum Strains Isolated From Malaysian Foods

“…Bacterial fermentation technology has gained vast attention as it produces compounds that have economic value for various applications such as biofuel, pharmaceutical products, and chemicals for various industries ( 32 ). Bacteria require sufficient carbon, nitrogen, and other nutrients in the growth medium to improve growth, metabolic activity, and production of vital metabolites ( 33 ). As reported previously, the optimization of fermentation media enhanced the bacteriocin inhibitory of postbiotic produced by L. plantarum I-UL4 ( 31 ).…”

Precision Manufacturing of Patterned Beryllium Bronze Leaf Springs via Chemical Etching

“…The Taguchi method was usually employed to obtain the optimal process parameters in different applications [16][17][18][19][20], which was also used in the present study. The roundness, straightness and offset of the leaf springs are regarded as three responsive variables.…”

Multivariate optimization of Staphylococcus xylosus AD1 biomass production using sugarcane molasses plus yeast extract and soybean meal

“…For the development of fermented meat products in the food industry, it is necessary to produce biomass by the multiplication of starter cultures. To enable the large-scale industrial production of starter cultures, low-cost culture media that ensure good bacterial growth are essential (Makowski et al, 2017). Obtained commercially, the medium that is currently used extensively for multiplication of Staphylococcus is Brain Heart Infusion broth (BHI) (Miranda, Sant'anna, & Porto, 1999).…”

“…It has been well established that bacterial cell biomass production is strongly influenced by the composition of media, including carbon, nitrogen, growth factors, and inorganic salts (Li, Bai, Cai, & Ouyang, 2002). Microorganisms, depending on their nutritional requirements, need access to different sources of constituents, and both a shortage and excess of even one component may inhibit the growth and activity of the cells, and consequently decrease the process efficiency of cell biomass production (Makowski et al, 2017). For this, due to the number of variables, optimization of media composition is a great challenge.…”