Centrifugal pumps as turbines (PATs) are widely used in chemical engineering for recycling the abundant energy from high-pressure fluid. The operation of PATs is significantly affected by their upstream conditions, which are not steady (i.e., with a constant flow rate) in reality, thus, research on the flow mechanism of PATs under transient conditions should be considered of higher importance. In this study, a numerical model of a PAT was developed by employing the sliding mesh method to describe turbine rotation, and a user-defined function (UDF) for characterizing transient flow conditions. Corresponding experiments were also conducted to provide validation results for the simulation, and the simulation results agreed well with the experimental outcomes. The instantaneous characteristic curves under the current working conditions were obtained for different transient flow rates. The results show that the turbine’s efficiency is significantly affected by transient flow conditions. In particular, a rapid increase (large time derivative) of flow rate results in a large energy dissipation at the turbine outlet, and therefore, the turbine efficiency decreases. In addition, as the flow rate increases, the hydrodynamic force on the impeller, and the pressure fluctuation amplitude in the volute first decrease and then increase, reaching the minimum near the design flow rate. The current study provides a reliable and precise approach for the estimation of hydrodynamic performance of fluid machinery under transient flow conditions.