Optimization of a 5-day fixed-time embryo transfer (FTET) protocol in heifers I. Manipulation of circulating progesterone through reutilization of intravaginal progesterone devices during FTET

Sala, R. V.; Melo, L. G. C.; Motta, J. C. Lemos; Leffers-Neto, L.; Carrenho-Sala, L. C.; Fosado, M.; Moreno, J. F.; Baruselli, Pietro Sampaio; Wiltbank, Milo C.; García-Guerra, A.

doi:10.1016/j.theriogenology.2020.06.002

Cited by 7 publications

(7 citation statements)

References 52 publications

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“…These heifers were most likely on d 1 or 2 of metestrus, characterized by serum P4 concentrations <0.50 ng/mL and absence of a CL >14 mm in diameter on d 0. In a previous study using a similar 5-d CIDR Synch protocol with a third or fourth use CIDR, time of ovulation averaged ~86 h after CIDR removal (Sala et al, 2020). In that study, 33.9% of the heifers ovulated before d 0 (personal communication, Rodrigo El Azzi et al | Replacing GnRH with hCG in a 5-day CIDR Synch Sala, ST Genetics, South Charleston, OH), which is lower than in the present study.…”

Section: Effects Of Human Chorionic Gonadotropin On the Last Day Of A...contrasting

confidence: 65%

“…The synchronization response in the present experiment indicates the proportion of heifers that would be on d 0, 1, or 2 of the estrous cycle on study d 0 and most likely to be used as recipients for ET on study d 6 and 7. Sala et al (2020), using a similar protocol with third use CIDR in Holstein heifers, also found a similar ovulatory response (97%) and an ET utilization ratio (transferred/treated) of 93.3%. In the same study, pregnancy per ET did not differ between heifers receiving ET 7 ± 1 d after detection of estrus and after a 5-d CIDR Synch protocol with a new or second use CIDR (Sala et al, 2020).…”

Section: Effects Of Human Chorionic Gonadotropin On the Last Day Of A...mentioning

confidence: 59%

“…Sala et al (2020), using a similar protocol with third use CIDR in Holstein heifers, also found a similar ovulatory response (97%) and an ET utilization ratio (transferred/treated) of 93.3%. In the same study, pregnancy per ET did not differ between heifers receiving ET 7 ± 1 d after detection of estrus and after a 5-d CIDR Synch protocol with a new or second use CIDR (Sala et al, 2020).…”

Section: Effects Of Human Chorionic Gonadotropin On the Last Day Of A...mentioning

confidence: 59%

“…Weekly cohorts of heifers were synchronized with a 5-d CIDR Synch protocol using a CIDR (1.38 g of P4, Eazi-Breed CIDR, Zoetis) previously used twice for 5 d each time. After each use, CIDR inserts were individually washed with water and soaked in a solution of 2% chlorhexidine diacetate (Novalsan Solution, Zoetis) for 15 min, as described by Sala et al (2020). The 5-d CIDR Synch protocol consisted of intravaginal insertion of a CIDR on d −8 and withdrawal 5 d later, followed by the administration of PGF 2α (500 mg of cloprostenol i.m.…”

Section: Effects Of Human Chorionic Gonadotropin On the Last Day Of A...mentioning

confidence: 99%

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Effects of human chorionic gonadotropin on the last day of a 5-day CIDR Synch protocol and 5 days later on circulating progesterone concentrations and luteal area in Holstein heifers

et al. 2022

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Section: Effects Of Human Chorionic Gonadotropin On the Last Day Of A...contrasting

confidence: 65%

Section: Effects Of Human Chorionic Gonadotropin On the Last Day Of A...mentioning

confidence: 59%

Section: Effects Of Human Chorionic Gonadotropin On the Last Day Of A...mentioning

confidence: 59%

Section: Effects Of Human Chorionic Gonadotropin On the Last Day Of A...mentioning

confidence: 99%

See 2 more Smart Citations

Effects of human chorionic gonadotropin on the last day of a 5-day CIDR Synch protocol and 5 days later on circulating progesterone concentrations and luteal area in Holstein heifers

et al. 2022

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“…A randomized controlled equivalence trial was conducted at STgenetics® Ohio Heifer Center facilities located in South Charleston Ohio USA, from November 2019 to May 2020 with a total of 151 animals enrolled in the study. Standard estrus synchronization protocols were used either for artificial insemination or embryo transfers ( Sala et al., 2020 ; Fig. 1 ).…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial prophylaxis post-amniocentesis procedures in cattle: A randomized controlled equivalence study

Absalón-Medina

Sala

Bond

2022

Veterinary and Animal Science

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Amniocentesis is a routine procedure utilized on several species including human, equine, and bovine patients. Early assessment and discovery of new genetic traits in the cattle industry are highly desirable in order to accelerate genetic gain by shortening generational intervals. One of the main concerns from this procedure is the introduction of pathogenic bacterial contamination into the amniotic cavity thereby increasing the risks of spontaneous pregnancy losses post procedure. In this randomized controlled equivalence study, we have tested the effect of antimicrobial prophylaxis on the incidence of spontaneous abortions and contrasted it to untreated individuals post amniocentesis. On the treated group ( n = 67) all heifers remained pregnant whereas 1 of the untreated group ( n = 65) resulted in a spontaneous abortion during the study period. The latter represents 1.54% of pregnancy losses attributed to the risk associated to the amniocentesis procedure. However, the probability of inducing spontaneous abortion from the technique itself is not different to that of the contemporaneous population ( n = 694) not undergoing amniocentesis viz., 1.59%. Following a two-tailed distribution, statistical analyses showed no significant differences across treatments (Fisher's exact test P = 0.49). The current prospective study indicates that performing amniocenteses on cattle have resulted in similar spontaneous pregnancy losses comparable to those of pregnant heifers without undergoing amniocentesis and regardless of antimicrobial use. In conclusion, prophylactic antimicrobials may not be applicable within the cattle amniocentesis framework.

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Embryo morphokinetics derived from fresh and vitrified bovine oocytes predict blastocyst development and nuclear abnormalities

Angel‐Velez

Coster

Azari‐Dolatabad

et al. 2023

Sci Rep

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Embryo development is a dynamic process and critical stages may go unnoticed with the use of traditional morphologic assessments, especially the timing of embryonic divisions and aberrant zygotic cleavage patterns. Bovine embryo development is impaired after oocyte vitrification, but little is known about the underlying morphokinetic behavior. Here, bovine zygotes from fresh (n = 708) and vitrified oocytes (n = 182) were monitored by time-lapse imaging and the timing and nature of early blastomere divisions were modeled to find associations with blastocyst development at day 8. The predictive potential of morphokinetic parameters was analyzed by logistic regression and receiver operating characteristic curve analysis to determine optimal cut-off values. Lag-phase was highly correlated with embryo development. Remarkably, 100% of zygotes that reached the blastocyst stage showed a lag-phase. Fast first cleavage increased the chance of blastocyst development to 30% with a cut-off of 32 h and 22 min. Aberrant zygotic cleavage events, including multipolar division, unequal blastomere sizes, and membrane ruffling resulted in decreased blastocyst development. Multipolar division leads to uneven blastomeres, which was associated with anuclear and multinuclear blastomeres, indicating genome segregation errors. Moreover, we described for the first time morphokinetics of embryos derived from vitrified bovine oocytes. Vitrification severely affected blastocyst development, although lower cryoprotectant concentration in equilibration solutions seems to be less detrimental for embryo yield. Impaired development was linked to slow cleavages, lower lag-phase incidence, and increased early embryonic arrest. Typically, less than 15% of the embryos produced from vitrified oocytes reached more than eight cells. Interestingly, the rate of abnormal first cleavage events was not affected by oocyte vitrification. In conclusion, time to first cleavage, the presence of a lag-phase, and the absence of aberrant zygotic cleavage were the best predictors of bovine blastocyst development for both fresh and vitrified oocytes.

show abstract

Optimization of a 5-day fixed-time embryo transfer (FTET) protocol in heifers I. Manipulation of circulating progesterone through reutilization of intravaginal progesterone devices during FTET

Cited by 7 publications

References 52 publications

Effects of human chorionic gonadotropin on the last day of a 5-day CIDR Synch protocol and 5 days later on circulating progesterone concentrations and luteal area in Holstein heifers

Effects of human chorionic gonadotropin on the last day of a 5-day CIDR Synch protocol and 5 days later on circulating progesterone concentrations and luteal area in Holstein heifers

Antimicrobial prophylaxis post-amniocentesis procedures in cattle: A randomized controlled equivalence study

Embryo morphokinetics derived from fresh and vitrified bovine oocytes predict blastocyst development and nuclear abnormalities

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