Optimization of 204 veterinary drug residues method and establishing their mass spectrum library

Abstract

A multi-class multi-residue analytical method was validated in this paper for simultaneously identifying 204 veterinary drugs in pork by UPLC-QTRAP MS/MS, including sulfonamides, quinolones, β-agonists, and nitroimidazoles. MRM-IDA-EPI mode of QTRAP was used to establish a secondary mass spectrum library of these 204 veterinary drugs, which could enhance the qualitative analysis of suspect substances. The separation of substances was performed on a C18 column, with a gradient elution of 0.1% formic acid solution in water and methanol. Electrospray ionization (ESI), fast polarity switching, and scheduled MRM mode were used to collect the characterization results. Factors that affected the recovery were delicately optimized, such as solvent proportion, formic acid content, and purification method. We finally determined the extracting method with formic acidacetonitrile-water (0.1:90:10, v:v:v) and purifying method with Cleanert LipoNo as a package for dispersive solid-phase extraction. Matrix effects on the quantitative results were also investigated. Some compounds had strong matrix inhibition or enhancement, and the matrix matching external standard method was used to quantify the compounds accurately. The correlation coefficient R 2 was over 0.99 within the calibration linearity range for the detected 204 veterinary drugs. The limit of quantification (LOQ) was 0.5 ~ 5 μg/kg. The recoveries ranged from 61.4% to 118.8% with RSDs of 3.1% to 19.2% (n = 6).

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“…Chloramphenicol residues in animal tissues had been detected with the resource of the use of GC with electron capture detector (ECD) (Cerkvenik, 2006). GC with ECD have become notably applied for the selfdiscipline of amitraz and its metabolite residues that have been confirmed with the resource of the use of fueloline chromatography-mass spectrometry (GC-MS) without derivatization (Tao et al, 2011) Lincomycin and spectinomycin had been determined with the resource of the use of GC with nitrogen-phosphorus detection (NPD) and confirmed with the resource of the use of GC-MS. GC isn't always often used for the self-discipline of veterinary tablets because of the requirement of derivatization (Li et al, 2020).…”

Targeted Analysis of Veterinary Drugs in Food Samples by Developing a High-Resolution Tandem Mass Spectral Library

“…Gómez-Pérez et al developed a high-resolution HR-MS library of 60 veterinary drugs for their identification in honey samples . Chen., D. et al and Li., X. et al developed a tandem mass spectral library of 400 and 204 veterinary drugs respectively using low-resolution mass spectrometers . Mass spectral libraries of pharmaceutical drugs also contain spectral information on several veterinary drugs. , The mass spectral data of some veterinary drugs exist in different spectral libraries, including “MassBank”, “Maurer/Wissenbach/Weber LC-MS library of drugs”, , MoNA (MassBank of North America), “Mass Spectra of Designer Drugs”, the “Wiley Registry of Mass Spectral Data”, and “NIST Tandem MS Library” .…”