Optimization Conditions for Maximum Oil Bioremediation and Biopolymer Production by Pseudomonads

Goudarztalejerdi, Ali; Tabatabaei, Mohammad; Eskandari, Mohammad Hadi; Mowla, Dariush

doi:10.1007/s40995-018-0652-3

Cited by 3 publications

(5 citation statements)

References 41 publications

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“…() and Goudarztalejerdi et al . (). GC‐MS was operated using Agilent 5977 mass spectrometer coupled to Agilent 7890B series gas chromatograph in electron impact mode.…”

Section: Methodsmentioning

confidence: 97%

“…The mixture was then vortexed for 1 min and left at room temperature for phase separation. The bottom phase containing methyl esters of corresponding fatty acid constituents was recovered and analysed by gas chromatography/mass spectrometry (GC-MS; Hewlett Packard GC-1450) using a protocol for hydroxyalkanoate methyl esters as described by Goudarztalejerdi et al (2015) and Goudarztalejerdi et al (2019). GC-MS was operated using Agilent 5977 mass spectrometer coupled to Agilent 7890B series gas chromatograph in electron impact mode.…”

Section: Effect Of Pah Concentration On Pah Degradation and Pha Produmentioning

confidence: 99%

“…The temperature of column was 80°C (maintained for 4 min), with an increase in 8°C to a temperature of 160°C (maintained for 6 min). Finally, the temperature was increased from 160 to 240°C and held for 5 min (Goudarztalejerdi et al 2015;Goudarztalejerdi et al 2019).…”

Section: Effect Of Pah Concentration On Pah Degradation and Pha Produmentioning

confidence: 99%

“…; Goudarztalejerdi et al . ). PHA, poly‐3‐hydroxydecanoate and poly‐3‐hydroxytetradecanoate, have been shown to exhibit thermal and mechanical properties that rival those of petroleum‐based polymers (Noda et al .…”

Section: Introductionmentioning

confidence: 97%

“…In this current study, mixed cultures of defined bacteria were isolated from a PAH-contaminated offshore site and evaluated for their ability to produce polyhydroxyalkanoate (PHA) using phenanthrene (PHE), pyrene (PYR) and fluoranthene (FLU) as substrates. The MCL-PHA produced were compared with those produced by other micro-organisms grown on PAH carbon sources (Narancic et al 2012;Goudarztalejerdi et al 2019). PHA, poly-3-hydroxydecanoate and poly-3-hydroxytetradecanoate, have been shown to exhibit thermal and mechanical properties that rival those of petroleum-based polymers (Noda et al 2005), and they were much better than those of a MCL-PHA consisting of a lower content of the C10 or C12 monomer (Wang et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Enhancing the degradation of mixed polycyclic aromatic hydrocarbon and medium‐chain‐length polyhydroxyalkanoate production by mixed bacterial cultures using modified repeated batch fermentation

et al. 2020

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Aims: To increase the biodegradation of phenanthrene (PHE), pyrene (PYR) and fluoranthene (FLU) through mixed cultures of polycyclic aromatic hydrocarbon (PAH)-degrading bacteria, using modified repeated batch fermentation. Methods and Results: Novel bacterial strains of Pseudomonas putida, Pseudomonas sp. and Ralstonia eutropha were cultivated and the biodegradation and conversion of mixed PAH to medium-chain-length polyhydroxyalkanoates (MCL-PHA) was determined. The highest degradation of PAH (100%) and PHA production (50Á0%) was obtained in medium containing 30 mmol l À1 of mixed PAH after three cycles of repeated batch fermentation. The concentration of PAH in the reactor was increased from 30 to 90 mmol l À1 with repeated additions of PAH, and bacteria were able to produce PHA at 40% of cell dry mass. The MCL-PHA were identified by gas chromatography/mass spectroscopy, with the 3-hydroxydecanoate (3-HD) monomer higher than 75 mol.%. Conclusions: This study demonstrated that the biodegradation of PHE, PYR and FLU was enhanced by modified repeated batch fermentation using a mixed culture of bacteria. In addition, this fermentation strategy also increased the production of PHA, with an increase in monomer composition. Significance and Impact of the Study: This was the first study to describe the enhancement of the degradation of mixed solutions of PHE, PYR and FLU, and PHA production, using novel mixed bacterial cultures and modified repeated batch fermentation. The MCL-PHA formed had uniquely high 3-HD content. * Poly(hydroxyoctanoate-co-hydroxyhexanoate) [P(HO-co-HX)]. † Poly(hydroxydecanoate-co-hydroxyoctanoate-co-hydroxyhexanoate) [P(HD-co-HO-co-HX)] obtained from Pseudomonas putida KT2442, cultivated under batch fermentation using 15 mmol l À1 sodium octanoate and 12 mmol l À1 decanoic acid as substrate. The data obtained from Escapa et al. (2011).

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“…() and Goudarztalejerdi et al . (). GC‐MS was operated using Agilent 5977 mass spectrometer coupled to Agilent 7890B series gas chromatograph in electron impact mode.…”

Section: Methodsmentioning

confidence: 97%

Section: Effect Of Pah Concentration On Pah Degradation and Pha Produmentioning

confidence: 99%

Section: Effect Of Pah Concentration On Pah Degradation and Pha Produmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%