Optimization and validation of LLE-LTP to determine florpyrauxifen-benzyl herbicide in water samples by HPLC-DAD

Vieira, Luís H. S.; Sicupira, Lázaro Chaves; Cacique, Ane Patrícia; Silvério, Flaviano Oliveira

doi:10.1080/03601234.2022.2099202

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…This value is classi ed as a very high matrix effect. This result was different from that found in a recent study using the same analysis and detection system (HPLC-DAD) for determining orpyrauxifen-benzyl in soil, as a low matrix effect was veri ed (-3.34%) (Lopes et al 2023) and there was no matrix effect in the analyzes of this herbicide for the water matrix (Vieira et al 2022).…”

Section: Matrix Effectcontrasting

confidence: 99%

“…Recent works studied the chromatographic conditions in detail to determine the orpyrauxifen-benzyl herbicide using HPLC-DAD for the water and soil matrices (Vieira et al 2022;Lopes et al 2023). The rice matrix extracts obtained through the optimized SLE-LTP were analyzed under the conditions proposed in these two studies.…”

Section: Optimization Of Chromatographic Conditionsmentioning

confidence: 99%

“…Therefore, there is a demand for developing new extraction methods which are easy to perform, with reduced use of samples and reagents, and that are sensitive and e cient. In turn, solid-liquid extraction with low temperature puri cation (SLE-LTP) has been successfully applied to several herbicides, including monitoring orpyrauxifen-benzyl in soil and water (Vieira et al 2022; Lopes et al 2023). This methodology has stood out for removing interferents which are trapped in the puri cation at low temperature, functioning as a previous clean-up of the extracts and streamlining the process, reducing the use of organic solvents and the nal cost ( Considering the representativeness of rice as one of the most consumed cereals in the world (EMBRAPA 2021), and the scarcity of studies involving the development of extraction methods for this herbicide, this study aimed to optimize and validate the SLE-LTP methodology to determine orpyrauxifen-benzyl herbicide in rice samples using high-performance liquid chromatography coupled with diode array detection (HPLC-DAD).…”

Section: Introductionmentioning

confidence: 99%

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Extraction Method for Determining Florpyrauxifen-Benzyl Herbicide in Rice

Lopes,

Sicupira,

Pinho

et al. 2023

Preprint

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Florpyrauxifen-benzyl is an active ingredient used as herbicide to control weeds in rice cultivation. So far, there are few studies related to the extraction and determining methodologies for this compound. Therefore, this work aimed to optimize and validate solid-liquid extraction with low temperature purification (SLE-LTP) using high-performance liquid chromatography coupled to a diode array detection (HPLC-DAD) to determine the florpyrauxifen-benzyl in rice samples. The results showed that the optimal chromatographic conditions were obtained using the kinetex column, temperature of 25 °C, mobile phase composition of methanol:water (78:22 v/v), flow rate of 0.3 mL min-1, and 260 nm as the wavelength. The optimum extraction conditions were obtained using acetonitrile and water (2:1) as extraction phase, followed by clean-up of extracts with alumina. The recovery percentage achieved was close to 100%, with relative standard deviation (RSD) less than 9.0%. The result showed a significant matrix effect (184.3%), but the methodology was precise, accurate, linear and selective, with a quantification limit of 5.0 μg kg-1.

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Section: Matrix Effectcontrasting

confidence: 99%

Section: Optimization Of Chromatographic Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Extraction Method for Determining Florpyrauxifen-Benzyl Herbicide in Rice

Lopes,

Sicupira,

Pinho

et al. 2023

Preprint

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Extraction Method for Determining Florpyrauxifen-Benzyl Herbicide in Rice

Lopes,

Sicupira,

Pinho

et al. 2024

Food Anal. Methods

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Florpyrauxifen-benzyl is an active ingredient used as herbicide to control weeds in rice cultivation. So far, there are few studies related to the extraction and determining methodologies for this compound. Therefore, this work aimed to optimize and validate solid-liquid extraction with low temperature puri cation (SLE-LTP) using high-performance liquid chromatography coupled to a diode array detection (HPLC-DAD) to determine the orpyrauxifen-benzyl in rice samples. The results showed that the optimal chromatographic conditions were obtained using the kinetex column, temperature of 25 °C, mobile phase composition of methanol:water (78:22 v/v), ow rate of 0.3 mL min -1 , and 260 nm as the wavelength.The optimum extraction conditions were obtained using acetonitrile and water (2:1) as extraction phase, followed by clean-up of extracts with alumina. The recovery percentage achieved was close to 100%, with relative standard deviation (RSD) less than 9.0%. The result showed a signi cant matrix effect (184.3%), but the methodology was precise, accurate, linear and selective, with a quanti cation limit of 5.0 μg kg -1 .

show abstract