Optimization and Validation of an Analytical Method for the Determination of Free and Hidden Fumonisins in Corn and Corn Products by UHPLC-MS/MS

Swift and Enhanced-Sensitive Analytical Method for Determination of Fumonisins FB1 And FB2 in Egyptian Oilseeds Using LC-MS/MS.

“…Also, the sensitivity was greater than the other columns, and the peak cheap's symmetric was great for both FB1 and FB2 figure 3. The run time in the present study was less than all the previous studies except de Matos et al, 2021 [34] it was so closed.…”

“…Also, the LOQ was lower than the value reported by Andrade et al, 2017 (19 and 8 µg/Kg for FB1 and FB2, respectively) [26]; Hu et al, 2019 (28 µg/Kg for FB1 and 27 µg/Kg for FB2) [25]. While the LOQ was closed related to de Matos et al, 2021 which ranged from 1.3 to 6.59 µg/Kg for FB1 and from 0.60 to 4.60 µg/Kg for FB2 [34]. In Franco et al 2018 LOQ was less than the present method for FB1 (2.8 µg/Kg) but highest in FB2 (2.5 µg/Kg) [33].…”

“…Also, in Franco et al 2018 the extraction solvent was ACN: Water: Acetic acid (80:20:0.1, v/v/v) with shaking for 90 min using the internal stander in the injection on LC-MS/MS [33], In Hu et al, 2019 the extraction solvent was ACN: Water: Acetic acid (70:29:1, v/v/v) with shaking for 30 minutes and using the lapelled internal stander in the injection on LC-MS/MS [25]. While the extraction solvent in de Matos et al, 2021 was ACN: Water: formic acid (75:24:1, v/v/v) with shaking for 20 min using the internal stander in the injection on LC-MS/MS [34]. These previous methods are based on the Dilute-and-Shoot technique with using the lapelled internal stander in the injection on LC-MS/MS.…”

“…In previous studies, there were many columns used to separate FB1 and FB2 in LC-MS/MS with different run Times. In Gazzotti et al 2011 [34].…”

“…Clean-up steps take a long time and may cause a loss of part of the target, which increases the cost of the method. On the other hand, studies based on dilution and injection techniques without clean-up steps showed fast, efficient, and reliable results [26,33,25,34]. Many investigations for the determination of the Fumonisin levels in various countries have been conducted [35,36,37].The last reported study on Fumonisin levels in Egypt has been performed in 2009 [38] which resulted in contamination and regulation violation of all samples with Fumonisin, although the a high risk of Fumonisin no more studies have been conducted since there.…”