Optimization and validation of a high performance liquid chromatography method for the simultaneous determination of vitamins A and E in human serum using monolithic column and diode-array detection

Urbánek, Lubor; Solichová, Dagmar; Melichar, Bohuslav; Dvořák, Josef; Svobodová, Iveta; Solich, Petr

doi:10.1016/j.aca.2006.02.032

Cited by 53 publications

(47 citation statements)

References 27 publications

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“…Serum alpha-tocopherol and retinol were determined before and during the therapy by high performance liquid chromatography as described ( 16 ). Blood samples were drawn from a peripheral vein after an overnight fast.…”

Section: Methodsmentioning

confidence: 99%

Serum Retinol, Alpha-Tocopherol and Systemic Inflammatory Response in Metastatic Colorectal Carcinoma Patients Treated with Combination Chemotherapy and Cetuximab

Melichar

Krčmová

Kalábová

et al. 2010

J Nutr Sci Vitaminol

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Summary Cetuximab is a chimeric antibody registered for the therapy of advanced colorectal carcinoma. Cancer and anticancer therapy are associated with oxidative stress, and disorders of antioxidant balance may be involved in the toxicity associated with anticancer treatment. The aim of the present study was to investigate the changes of serum retinol, alpha-tocopherol and C-reactive protein during the first month of treatment with cetuximab and chemotherapy. Twenty-five consecutive patients with metastatic colorectal carcinoma treated with a combination of chemotherapy and cetuximab were included in the present study. Serum retinol and alpha-tocopherol were determined by high-performance liquid chromatography and serum C-reactive protein was determined using commercial kits. Significant correlation was observed between baseline concentrations of retinol and C-reactive protein ( r s ϭϪ 0.54, p Ͻ 0.01). Median survival of patients who had baseline serum retinol below 1.25 mol/L was 10 mo compared to 18 mo for patients who had serum retinol equal or above 1.25 mol/L ( p Ͻ 0.05); median survival of patients who had serum C-reactive protein below 24 mg/L was significantly longer compared to patients with C-reactive protein levels equal or above 24 mg/L (18 vs. 7 mo, p Ͻ 0.05), but no difference in survival was observed based on alpha-tocopherol levels. Twenty-two patients had evaluation of retinol, alpha-tocopherol and C-reactive protein at least once during the follow up. Serum concentration of alpha-tocopherol decreased significantly during the therapy, but retinol and Creactive protein concentrations remained unchanged. In conclusion, a significant correlation was observed between serum retinol and C-reactive protein. Serum alpha-tocopherol decreased significantly during the first month of combination therapy with cetuximab. Low retinol and high C-reactive protein concentrations were predictive of poor prognosis in this patient population.

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Section: Methodsmentioning

confidence: 99%

Serum Retinol, Alpha-Tocopherol and Systemic Inflammatory Response in Metastatic Colorectal Carcinoma Patients Treated with Combination Chemotherapy and Cetuximab

Melichar

Krčmová

Kalábová

et al. 2010

J Nutr Sci Vitaminol

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“…Analysis of plasma retinol was performed by isocratic phase HPLC as described previously (Urbanek et al 2006). Briefly, internal standard solution (a-tocopherol) and plasmatic retinol were extracted using hexane solution supplemented with butylated hydroxytoluene (BHT) as an antioxidant.…”

Section: Plasma Retinolmentioning

confidence: 99%

Vitamin A regulates hypothalamic–pituitary–adrenal axis status in LOU/C rats

Marissal-Arvy¹,

Hamiani²,

Richard³

et al. 2013

Journal of Endocrinology

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The aim of this study was to explore the involvement of retinoids in the hypoactivity and hyporeactivity to stress of the hypothalamic-pituitary-adrenal (HPA) axis in LOU/C rats. We measured the effects of vitamin A deficiency administered or not with retinoic acid (RA) on plasma corticosterone in standard conditions and in response to restraint stress and on hypothalamic and hippocampal expression of corticosteroid receptors, corticotropinreleasing hormone and 11b-hydroxysteroid dehydrogenase type 1 (11b-HSD1) in LOU/C rats. Interestingly, under control conditions, we measured a higher plasma concentration of retinol in LOU/C than in Wistar rats, which could contribute to the lower basal activity of the HPA axis in LOU/C rats. Vitamin A deficiency induced an increased HPA axis activity in LOU/C rats, normalized by RA administration. Compared with LOU/C control rats, vitamin A-deficient rats showed a delayed and heightened corticosterone response to restraint stress. The expression of corticosteroid receptors was strongly decreased by vitamin A deficiency in the hippocampus, which could contribute to a less efficient feedback by corticosterone on HPA axis tone. The expression of 11b-HSD1 was increased by vitamin A deficiency in the hypothalamus (C62.5%) as in the hippocampus (C104.7%), which could lead to a higher production of corticosterone locally and contribute to alteration of the hippocampus. RA supplementation treatment restored corticosterone concentrations and 11b-HSD1 expression to control levels. The high vitamin A status of LOU/C rats could contribute to their low HPA axis activity/reactivity and to a protective effect against 11b-HSD1-mediated deleterious action on cognitive performances during ageing.

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“…Serum and lipoprotein alpha-tocopherol was determined by high performance liquid chromatography as described ( 20 ). In the liquid-liquid extraction procedure, 500 L of sample consisting of either serum or lipoprotein fraction was de-proteinized by cool ethanol denatured with 5% methanol (500 L, 5 min, 4˚C) for the determination of alpha-tocopherol.…”

Section: Methodsmentioning

confidence: 99%

Erythrocyte Alpha-Tocopherol in Breast Cancer Patients Treated with Letrozol

Melichar

Plíšek

Hyšpler

et al. 2010

J Nutr Sci Vitaminol

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SummaryIn an earlier study, we have observed an increase of alpha-tocopherol in breast cancer patients treated with third-generation aromatase inhibitors that was related to tamoxifen withdrawal. We report here the results of measurement of alpha-tocopherol in erythrocytes and alpha-tocopherol/cholesterol ratios in patients treated with letrozol. Alpha-tocopherol in lipoprotein fractions and erythrocytes was determined by high-performance liquid chromatography in 124 post-menopausal women with breast cancer treated with letrozol immediately before the start of treatment as well as 2 and 4 mo later. After a transient decrease after 2 mo of letrozol therapy, erythrocyte alpha-tocopherol concentrations returned to pre-treatment levels 4 mo after the start of treatment. Apart from lower cholesterol in patients pre-treated with tamoxifen, no significant differences were observed at baseline between patients previously treated with tamoxifen and patients who had no prior tamoxifen in any of the other parameters investigated, but the transient decrease of erythrocyte alpha-tocopherol was observed only in patients previously treated with tamoxifen. Alpha-tocopherol content of lipoprotein fractions was significantly increased 4 mo after the start of therapy, but this increase was evident mostly in patients not treated earlier with tamoxifen. In conclusion, only minor changes of alpha-tocopherol, including a transient decrease of alpha-tocopherol in erythrocyte membranes, and an increase of alphatocopherol in lipoprotein fractions were observed during the first 4 mo of letrozol therapy.

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Optimization and validation of a high performance liquid chromatography method for the simultaneous determination of vitamins A and E in human serum using monolithic column and diode-array detection

Cited by 53 publications

References 27 publications

Serum Retinol, Alpha-Tocopherol and Systemic Inflammatory Response in Metastatic Colorectal Carcinoma Patients Treated with Combination Chemotherapy and Cetuximab

Serum Retinol, Alpha-Tocopherol and Systemic Inflammatory Response in Metastatic Colorectal Carcinoma Patients Treated with Combination Chemotherapy and Cetuximab

Vitamin A regulates hypothalamic–pituitary–adrenal axis status in LOU/C rats

Erythrocyte Alpha-Tocopherol in Breast Cancer Patients Treated with Letrozol

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