Optimization and validation of a multiplex assay for microsatellite loci analysis in the field cricket, Gryllus campestris (Orthoptera: Gryllidae)

Panagiotopoulou, Hanna; Baca, Mateusz; Baca, Katarzyna; Stanković, Anna; Żmihorski, Michał

doi:10.1016/j.aspen.2015.04.009

Cited by 2 publications

(2 citation statements)

References 29 publications

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“…Therefore, concurrent amplification of markers in multiplex polymerase chain reactions (PCR) is desirable because this procedure will significantly shorten the time of laboratory work and reduce the cost of analysis. Such assays need to be first optimized and validated to justify the use of the loci in future research projects 12 – 14 .…”

Section: Introductionmentioning

confidence: 99%

Development of microsatellite loci and optimization of a multiplex assay for Latibulus argiolus (Hymenoptera: Ichneumonidae), the specialized parasitoid of paper wasps

Kostro‐Ambroziak

Siekiera

Czajkowska

et al. 2020

Sci Rep

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Microsatellite loci are commonly used markers in population genetic studies. In this study, we present 40 novel and polymorphic microsatellite loci elaborated for the ichneumonid parasitoid Latibulus argiolus (Rossi, 1790). Reaction condition optimisation procedures allowed 14 of these loci to be co-amplified in two PCRs and loaded in two multiplex panels onto a genetic analyser. The assay was tested on 197 individuals of L. argiolus originating from ten natural populations obtained from the host nests of paper wasps. The validated loci were polymorphic with high allele numbers ranging from eight to 27 (average 17.6 alleles per locus). Both observed and expected heterozygosity values were high, ranging between 0.75 and 0.92 for HO (mean 0.83) and from 0.70 to 0.90 for HE (mean 0.85). The optimized assay showed low genotyping error rate and negligible null allele frequency. The designed multiplex panels could be successfully applied in relatedness analyses and genetic variability studies of L. argiolus populations, which would be particularly interesting considering the coevolutionary context of this species with its social host.

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Section: Introductionmentioning

confidence: 99%

Development of microsatellite loci and optimization of a multiplex assay for Latibulus argiolus (Hymenoptera: Ichneumonidae), the specialized parasitoid of paper wasps

Kostro‐Ambroziak

Siekiera

Czajkowska

et al. 2020

Sci Rep

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show abstract

“…Gbim59 and Gbim71 (Dawson et al, 2003;Bretman et al, 2008) were amplifi ed, divided into 4 multiplex reactions (Panagiotopoulou et al, 2015). Capillary electrophoresis of PCR products, divided into two panels, was conducted in Oligo.pl service (IBB, Warsaw, Poland) on an ABI PRISM 3730 Genetic Analyzer (Applied Biosystems, Carlsbad, CA, USA).…”

Section: Dna Extraction Amplifi Cation and Sequencingmentioning

confidence: 99%

Genetic identification of a non-native species introgression into wild population of the field cricket Gryllus campestris (Orthoptera: Gryllidae) in Central Europe

Panagiotopoulou¹,

Baca²,

Baca³

et al. 2016

Eur. J. Entomol.

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Optimization and validation of a multiplex assay for microsatellite loci analysis in the field cricket, Gryllus campestris (Orthoptera: Gryllidae)

Cited by 2 publications

References 29 publications

Development of microsatellite loci and optimization of a multiplex assay for Latibulus argiolus (Hymenoptera: Ichneumonidae), the specialized parasitoid of paper wasps

Development of microsatellite loci and optimization of a multiplex assay for Latibulus argiolus (Hymenoptera: Ichneumonidae), the specialized parasitoid of paper wasps

Genetic identification of a non-native species introgression into wild population of the field cricket Gryllus campestris (Orthoptera: Gryllidae) in Central Europe

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